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Pre-adipocyte substrain of 3T3 (Swiss albino) developed through clonal isolation. Cells undergo a conversion to adipose-like cells as they progress from a rapidly dividing to a confluent and contact inhibited state. (Green & Meuth (1974) Cell 3: 127-133)

Cell Type:
Fibroblast
Tissue Origin:
embryo
Species:
mouse
Research Area:
Cancer Research/Cell Biology
Cell Characteristics:
Adherent

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
SE CM-137 1e6 97% 74-84% Plasmid (general) 2 µg 100 µl 4D X-Unit
SE CM-137 2e5 99% 62-70% Plasmid (general) 400 ng 20 µl 4D X-Unit
V T-030 1e6 76-79% 52-66% Plasmid (general) 2 µg 100 µl I/II/2b

Citations (11)

A biomimetic hydrogel functionalized with adipose ECM components as a microenvironment for the 3D culture of human and murine adipocytes.
Categories:
3D Cell Culture  
Authors:
Louis F, Pannetier P, Souguir Z, Le Cerf D, Valet P, Vannier JP, Vidal G, Demange E 
In:
Biotechnol Bioeng (2017) 114(8): 1813-1824 
KDT501, a derivative from hops, normalizes glucose metabolism and body weight in rodent models of diabetes
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Konda VR, Desai A, Darland G, Grayson N, Bland JS 
In:
PLoS ONE (2014) 9(1): ePub 
Apelin inhibits diet-induced obesity by enhancing lymphatic and blood vessel integrity
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Sawane M, Kajiya K, Kidoya H, Takagi M, Muramatsu F, Takakura N. 
In:
Diabetes (2013) 62(6): 1970-1980 
Bifunctional Role of Rev-erb alpha in Adipocyte Differentiation
Categories:
Transfection 
Authors:
Wang J, Lazar MA 
In:
Mol Cell Biol (2008) 28(7): 2213-20 
Genetic and pharmacological inhibition of Rho-associated kinase II enhances adipogenesis
Categories:
Transfection 
Authors:
Noguchi M, Hosoda K, Fujikura J, Fujimoto M, Iwakura H, Tomita T, Ishii T, Arai N, Hirata M, Ebihara K, Masuzaki H, Itoh H, Narumiya S, Nakao K 
In:
J Biol Chem (2007) 282(40): 29574-83 
Impaired adipogenesis caused by a mutated thyroid hormone alpha1 receptor
Categories:
Transfection 
Authors:
Ying H, Araki O, Furuya F, Kato Y, Cheng SY 
In:
Mol Cell Biol (2007) 27(6): 2359-71 
Role of the Toll-like Receptor 4/NF-B Pathway in Saturated Fatty Acid-induced Inflammatory Changes in the Interaction Between Adipocytes and Macrophages
Categories:
Transfection 
Authors:
Suganami T, Tanimoto-Koyama K, Nishida J, Itoh M, Yuan X, Mizuarai S, Kotani H, Yamaoka S, Miyake K, Aoe S, Kamei Y, Ogawa Y 
In:
Arterioscler Thromb Vasc Biol (2007) 27(1): 84-91 
Testosterone inhibits adipogenic differentiation in 3T3-L1 cells: nuclear translocation of androgen receptor complex with beta-catenin and T-cell factor 4 may bypass canonical Wnt signaling to down-regulate adipogenic transcription factors
Categories:
Transfection 
Authors:
Singh R, Artaza JN, Taylor WE, Braga M, Yuan X, Gonzalez-Cadavid NF and Bhasin S 
In:
Endocrinology (2006) 147(1): 141-154 
Mitochondrial dysfunction induces triglyceride accumulation in 3T3-L1 cells: role of fatty acid -oxidation and glucose
Categories:
Transfection 
Authors:
Vankoningsloo S, Piens M, Lecocq C, Gilson A, De Pauw A, Renard P, Demazy C, Houbion A, Raes M and Arnould T 
In:
J Lipid Res (2005) 46(6): 1133-1149 
Fad24, a mammalian homolog of Noc3p, is a positive regulator in adipocyte differentiation
Categories:
Transfection 
Authors:
Tominaga K, Johmura Y, Nishizuka M and Imagawa M 
In:
J Cell Sci (2004) 117(Pt 25): 6217-6226 
The novel gene fad104, containing a fibronectin type III domain, has a significant role in adipogenesis
Categories:
Transfection 
Authors:
Tominaga K, Kondo C, Johmura Y, Nishizuka M, Imagawa M 
In:
FEBS Lett (2004) 577(1-2): 49-54 
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