Efficient transient genetic labeling of human CD34+ progenitor cells for in vivo application

Authors:
Wiehe JMI, Niesler C, Torzowski J, Zimmermann O, Wiesneth M, Schmitt M, Schwarz K, Dohner H, Hombach V Greiner J
In:
Source: Regenerative Med
Publication Date: (2006)
Issue: 1(2): 223-234
Research Area:
Immunotherapy / Hematology
Cells used in publication:
CD34+ cell, human
Species: human
Tissue Origin: blood
MUTZ-2
Species: human
Tissue Origin: blood
Platform:
Nucleofectorâ„¢ I/II/2b
Experiment
Transfected CD34+ and Mutz2 cells (a human acute myeloid leukemia)and then differentiated them into granulocytes, macrophages and megakaryocytes. CD34+ (but not Mutz2) cells could also be differentiated into erythrocytes.
Abstract