cells

Human acute myeloid leukemia, established from the peripheral blood of a 62-year-old man (AML FAB M2) at diagnosis in 1993

Cell Type:

Myeloid (unspec.)

Tissue Origin:

blood

Species:

human

Research Area:

Immunotherapy / Hematology

Cell Characteristics:

Suspension

Recommended Media

MEM Alpha Eagle

MEM Alpha was developed in 1971 and differs from standard MEM by containing the following unique components: vitamin B12, ascorbic acid, non-essential amino acids, sodium pyruvate, lipoic acid, and D-biotin.
This media has been used to culture bone marrow cells and amniotic cells for chromosome analysis.

MEM Alpha is available as:
12-169 - without L-glutamine, deoxyribonucleosides or ribonucleosides
BE02-002 - with Ultraglutamine, deoxyribonucleosides and ribonucleosides

Transfection Information

Lonza Optimized Protocol
Optimization Guideline

Filter:

The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.

Protocol	Kit	Program	Cells	Efficiency	Viable Cells	Substrate		Format	Platform
	V	G-009	2e6	55%		Plasmid (general)	2 µg	100 µl	I/II/2b

Citations

Efficient transient genetic labeling of human CD34+ progenitor cells for in vivo application

Categories:

Transfection

Authors:

Wiehe JMI, Niesler C, Torzowski J, Zimmermann O, Wiesneth M, Schmitt M, Schwarz K, Dohner H, Hombach V Greiner J

In:

Regenerative Med (2006) 1(2): 223-234

MUTZ-2

Recommended Media

Transfection Information

Citations