LINGO-1 is a component of the Nogo-66 receptor/p75 signaling complex
Mi S, Lee X, Shao Z, Thill G, Ji B, Relton J, Levesque M, Allaire N, Perrin S, Sands B, Crowell T, Cate RL, McCoy JM and Pepinsky RB
Cells used in publication:
Neuron, hippo/cortical, rat
Tissue Origin: brain
Primary P7 rat culture cerebellar granular neurons were nucleofected with the dominant negative form DN-LINGO-1, lacking the cytoplasmic domain involved in binding the Nogo-66 receptor /p75 signaling complex. DN-LINGO-1 transfected neurons built longer axons due to a diminished responsiveness to myelin inhibition. In the absence of myelin, no axon outgrowth could be observed. The cells also showed a decreased level of RhoA activation.
Axon regeneration in the adult CNS is prevented by inhibitors in myelin. These inhibitors seem to modulate RhoA activity by binding to a receptor complex comprising a ligand-binding subunit (the Nogo-66 receptor NgR1) and a signal transducing subunit (the neurotrophin receptor p75). However, in reconstituted non-neuronal systems, NgR1 and p75 together are unable to activate RhoA, suggesting that additional components of the receptor may exist. Here we describe LINGO-1, a nervous system-specific transmembrane protein that binds NgR1 and p75 and that is an additional functional component of the NgR1/p75 signaling complex. In non-neuronal cells, coexpression of human NgR1, p75 and LINGO-1 conferred responsiveness to oligodendrocyte myelin glycoprotein, as measured by RhoA activation. A dominant-negative human LINGO-1 construct attenuated myelin inhibition in transfected primary neuronal cultures. This effect on neurons was mimicked using an exogenously added human LINGO-1-Fc fusion protein. Together these observations suggest that LINGO-1 has an important role in CNS biology.
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