L-428

Hodgkin lymphoma
Cell Type:
Leukocyte (unspec.)
Tissue Origin:
blood
Species:
human
Research Area:
Cancer Research/Cell Biology
Immunotherapy / Hematology
Cell Characteristics:
Suspension

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
SF EW-106 4e5 62% ±8 64% ±8 Plasmid (general) 0.4 µg 20 µl 4D X-Unit
SF EW-106 2e6 67% ±9 65% ±8 Plasmid (general) 2 µg 100 µl 4D X-Unit
L X-001 1e6 77.6% ±3 73.2% ±7 Plasmid (general) 2 µg 100 µl I/II/2b

Citations

Categories:
Transfection 
Authors:
Hirsch B, Hummel M, Bentink S, Fouladi F, Spang R, Zollinger R, Stein H, Dürkop H 
In:
Am J Pathol (2008) 172(2): 510-20 
Categories:
Transfection 
Authors:
Leppik L, Gunst K, Lehtinen M, Dillner J, Streker K, de Villiers EM 
In:
J Virol (2007) 81(17): 9346-56 
Categories:
Transfection 
Authors:
Ushmorov A, Leithauser F, Sakk O, Weinhausel A, Popov SW, Moller P, Wirth T 
In:
Blood (2006) 107(6): 2493-500