ARPE-19

retinal pigmented epithelium

Cell Type:
Eye Epith.
Tissue Origin:
eye
Species:
human
Cell Characteristics:
Adherent

Recommended Media

DMEM:F12 combines the richness of F12 with the higher component concentrations of DMEM. This medium is well suited for clonal density cultures and has been extensively used to demonstrate the effect of various hormones and growth factors on target tissues.

Storage = 2ºC to 8ºC

12-719 = 3.151 g/L glucose, L-glutamine and 15mM HEPES
BE04-687Q = 3.151 g/L glucose and L-glutamine, without HEPES
BE04-687F/U1 = 3.151 g/L glucose and Ultraglutamine I, without HEPES

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
V X-001 1e6 80-86% 89-95% Plasmid (general) 2 µg 100 µl I/II/2b
SF DN-100 2e5 95% 84-94% Plasmid (general) 0.4 20 µl 4D X-Unit
SF DN-100 1e6 96-100% 75-100% Plasmid (general) 2 µg 100 µl 4D X-Unit

Citations

Categories:
Transfection 
Authors:
Wang W, Stukenberg PT, Brautigan DL 
In:
Mol Biol Cell (2008) epub: online 
Categories:
Transfection 
Authors:
Liu B, Li Z, Mahesh SP, Kurup SK, Giam CZ, Nussenblatt RB 
In:
Invest Ophthalmol Vis Sci (2006) 47(4): 1510-5