ARPE-19
Export
retinal pigmented epithelium
Cell Type:
Eye Epith.
Tissue Origin:
eye
Species:
human
Cell Characteristics:
Adherent
Transfection Information
Lonza Optimized Protocol
Optimization Guideline
Filter:
Any Substrate
Any Platform
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols
or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our
optimization strategy
to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol
Kit
Program
Cells
Efficiency
Viable Cells
Substrate
Format
Platform
V
X-001
1e6
80-86%
89-95%
Plasmid (general)
2 µg
100 µl
I/II/2b
SF
DN-100
2e5
95%
84-94%
Plasmid (general)
0.4
20 µl
4D X-Unit
SF
DN-100
1e6
96-100%
75-100%
Plasmid (general)
2 µg
100 µl
4D X-Unit
Citations
Phosphatase Inhibitor-2 Balances Protein Phosphatase 1 and Aurora B Kinase for Chromosome Segregation and Cytokinesis in Human Retinal Epithelial Cells
Categories:
Transfection
Authors:
Wang W, Stukenberg PT, Brautigan DL
In:
Mol Biol Cell (2008) epub: online
HTLV-1 Infection of Human Retinal Pigment Epithelial Cells and Inhibition of Viral Infection by an Antibody to ICAM-1
Categories:
Transfection
Authors:
Liu B, Li Z, Mahesh SP, Kurup SK, Giam CZ, Nussenblatt RB
In:
Invest Ophthalmol Vis Sci (2006) 47(4): 1510-5