MDA-MB-231

Breast adenocarcinoma cells

Cell Type:
Mammary Epith.
Tissue Origin:
breast
Species:
human
Research Area:
Cancer Research/Cell Biology
Cell Characteristics:
Adherent

Recommended Media

L-15 (Leibovitz) Medium was developed (1963) for fast growing tumor cells and was formulated for use without CO2 enriched atmosphere. Its unique features include: free base forms of arginine, cysteine and histidine; high concentration of tyrosine; galactose used instead of glucose and no sodium bicarbonate.
L-15 is used to grow cell lines in the absence of CO2 enriched atmosphere.  The bicarbonate-free medium is buffered with elevated levels of amino acids.

Storage = 2ºC to 8ºC

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
SE CH-125 4e5 72% ±1 64% ±7 Plasmid (general) 0.4 µg 20 µl 4D X-Unit
SE CH-125 2e6 75% ±1 67% ±3 Plasmid (general) 2 µg 100 µl 4D X-Unit
V X-013 1e6 77-81% 76-78% Plasmid (general) 2 µg 100 µl I/II/2b

Citations (18)

Categories:
Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Ray A, Vasudevan S, Sengupta S 
In:
PLoS ONE (2015) 10(9): e0137614 
Categories:
Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Chen L, Du-Cuny L, Moses S, Dumas S, Song Z, Rezaeian AH, Lin HK, Meuillet EJ, Zhang S 
In:
Other (2015) 11(1): e1004021 
Categories:
Transfection 
Authors:
Yanagisawa M, Huveldt D, Kreinest P, Lohse CM, Cheville JC, Parker AS, Copland JA, Anastasiadis PZ 
In:
J Biol Chem (2008) 283(26): 18344-54 
Categories:
Transfection 
Authors:
Pervin S, Tran AH, Zekavati S, Fukuto JM, Singh R, Chaudhuri G 
In:
Cancer Res (2008) 68(12): 4862-74 
Categories:
Transfection 
Authors:
Bicaku E, Marchion DC, Schmitt ML, Münster PN 
In:
Cancer Res (2008) 68(5): 1513-9 
Categories:
Transfection 
Authors:
Seng S, Avraham HK, Jiang S, Yang S, Sekine M, Kimelman N, Li H, Avraham S 
In:
Cancer Res (2007) 67(18): 8596-604 
Categories:
Transfection 
Authors:
Trauernicht AM, Kim SJ, Kim NH, Boyer TG 
In:
Mol Endocrinol (2007) 21(7): 1526-36 
Categories:
Transfection 
Authors:
Shibata MA, Miwa Y, Morimoto J, Otsuki Y 
In:
Med Mol Morphol (2007) 40(2): 103-7 
Categories:
Transfection 
Authors:
Yi CH, Smith DJ, West WW, Hollingsworth MA 
In:
Am J Pathol (2007) 170(5): 1535-45 
Categories:
Transfection 
Authors:
Hotary K, Li XY, Allen E, Stevens SL, Weiss SJ 
In:
Genes Dev (2006) 20(19): 2673-86 
Categories:
Transfection 
Authors:
Mitsui Y, Mochizuki S, Kodama T, Shimoda M, Ohtsuka T, Shiomi T, Chijiiwa M, Ikeda T, Kitajima M, Okada Y 
In:
Cancer Res (2006) 66(20): 9913-20 
Categories:
Transfection 
Authors:
Winnard PT Jr, Kluth JB, Raman V 
In:
Neoplasia (2006) 8(10): 796-806 
Categories:
Transfection 
Authors:
Cuevas BD, Winter-Vann AM, Johnson NL, Johnson GL 
In:
Oncogene (2006) 25(36): 4998-5010 
Categories:
Transfection 
Authors:
Betapudi V, Licate LS, Egelhoff TT 
In:
Cancer Res (2006) 66(9): 4725-33 
Categories:
Transfection 
Authors:
Morris DR, Ding Y, Ricks TK, Gullapalli A, Wolfe BL and Trejo J 
In:
Cancer Res (2006) 66(1): 307-314 
Categories:
Transfection 
Authors:
Shibata MA, Miwa Y, Miyashita M, Morimoto J, Abe H and Otsuki Y 
In:
Cancer Sci (2005) 96(7): 434-440 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
James CO, Huang MB, Khan M, Garcia-Barrio M, Powell MD, Bond VC 
In:
J Virol (2004) 78(6): 3099-109 
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