NALM-6
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human B cell precursor leukemia
Cell Type:
B cell
Tissue Origin:
blood
Species:
human
Research Area:
Cancer Research/Cell Biology
Immunotherapy / Hematology
Cell Characteristics:
Suspension
Transfection Information
Lonza Optimized Protocol
Optimization Guideline
Filter:
Any Substrate
Any Platform
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols
or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our
optimization strategy
to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol
Kit
Program
Cells
Efficiency
Viable Cells
Substrate
Format
Platform
SF
CV-104
2e5
71-79%
75-99%
Plasmid (general)
0.4 µg
20 µl
4D X-Unit
SF
CV-104
1e6
77-85%
66-76%
Plasmid (general)
2 µg
100 µl
4D X-Unit
T
C-005
2e6
57-71%
83-91%
Plasmid (general)
2 µg
100 µl
I/II/2b
Citations
Fate induction in CD8 CAR T cells through asymmetric cell division
Categories:
Transfection
Authors:
Lee CS, Chen S, Berry CT, Kelly AR, Herman PJ, Oh S, O'Connor RS, Payne AS, Ellebrecht CT
In:
Nature (2024) :
Nucleofection-based gene targeting in human pre-B cells.
Categories:
Transfection
Authors:
Kurosawa A, Saito S, Mori M, Adachi N.
In:
Gene Ther (2012) 492 (1): 305-8
Cloning of genes involved in chromosomal translocations by high-resolution single nucleotide polymorphism genomic microarray
Categories:
Transfection
Authors:
Kawamata N, Ogawa S, Zimmermann M, Niebuhr B, Stocking C, Sanada M, Hemminki K, Yamatomo G, Nannya Y, Koehler R, Flohr T, Miller CW, Harbott J, Ludwig WD, Stanulla M, Schrappe M, Bartram CR, Koeffler HP
In:
Proc Natl Acad Sci USA (2008) 105(33): 11921-6
Integrin inhibition through Lyn-dependent cross talk from CXCR4 chemokine receptors in normal human CD34+ marrow cells
Categories:
Transfection
Authors:
Nakata Y, Tomkowicz B, Gewirtz AM, Ptasznik A
In:
Blood (2006) 107(11): 4234-9
Analysis of folylpoly-gamma-glutamate synthetase gene expression in human B-precursor ALL and T-lineage ALL cells
Categories:
Transfection
Authors:
Leclerc GJ, Leclerc GM, Kinser TT, Barredo JC
In:
BMC Cancer (2006) 6: 132