SMC, pul.artery (PASMC), human

Primary SMC isolated from human pulmonary artery

Cell Type:
Smooth muscle cell
Tissue Origin:
artery
Species:
human
Research Area:
Cardiovascular
Cell Characteristics:
Adherent

Recommended Media

 SmGM-2 was developed for the maintenance and expansion of human smooth muscle cells. This medium contains 5% FBS.

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
Smooth muscle, basic A-033 2e6 90% 85% Plasmid (general) 2 µg 100 µl I/II/2b
P1

FG-113

1e5 85% 55% Plasmid (general) 0.2 µg 20 µl 4D X-Unit
P1 FG-113 1e5 85% 55% Plasmid (general) 0.2 µg 20 µl Shuttle

Citations (26)

Categories:
Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Liu JF, Han Z, Han ZC, HeZX 
In:
Other (2014) 20(8): 3411-3439 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Bauer EM, Zheng H, Lotze MT, Bauer PM. 
In:
PLoS ONE (2014) 9(5): e96720 
Categories:
Primary Cells and Media 
Authors:
Yu HJ1, Serebryannyy LA, Fry M, Greene M, Chernaya O, Hu WY, Chew TL, Mahmud N, Kadkol SS, Glover S, Prins G, Strakova Z, de Lanerolle P 
In:
PLoS ONE (2013) 8(11): e79776 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Abrahamsen I, Lorens JB 
In:
BMC Cell Biol (2013) 14:36: 1-7 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Solodushko V, Khader HA, Fouty BW 
In:
PLoS ONE (2013) 8(8): e71490 
Categories:
Primary Cells and Media 
Authors:
Liu G, Li X, Li Y, Tang X, Xu J, Li R, Hao P, Sun Y. 
In:
Biomed Res Int. (2013) 2013: 903947 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Chen B, Calvert AE, Meng X, Nelin LD 
In:
Am J Respir Cell Mol Biol (2012) 47(2): 218-26 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Raghavan A, Zhou G, Zhou Q, Ibe JC, Ramchandran R, Yang Q, Racherla H, Raychaudhuri P, Raj JU 
In:
Am J Respir Cell Mol Biol (2012) 46(4): 431-6 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Drake KM, Zygmunt D, Mavrakis L, Harbor P, Wang L, Comhair SA, Erzurum SC, Aldred MA 
In:
Am J Respir Crit Care Med (2011) 184(12): 1400-8 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Wu C, So J, Davis-Dusenbery BN, Qi HH, Bloch DB, Shi Y, Lagna G, Hata A 
In:
Mol Cell Biol (2011) 31(23): 4760-74 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Hartney T, Birari R, Venkataraman S, Villegas L, Martinez M, Black SM, Stenmark KR, Nozik-Grayck E. 
In:
PLoS ONE (2011) 6(11): e27531 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media, Transfection 
Authors:
Perez VA, Ali Z, Alastalo TP, Ikeno F, Sawada H, Lai YJ, Kleisli T, Spiekerkoetter E, Qu X, Rubinos LH, Ashley E, Amieva M, Dedhar S, Rabinovitch M 
In:
J Cell Biol (2011) 192(1): 171-88 
Categories:
Primary Cells and Media 
Authors:
Falcetti E, Hall SM, Phillips PG, Patel J, Morrell NW, Haworth SG, Clapp LH 
In:
Am J Respir Crit Care Med (2010) 182(9): 1161-70 
Categories:
Primary Cells and Media 
Authors:
Aldred MA, Comhair SA, Varella-Garcia M, Asosingh K, Xu W, Noon GP, Thistlethwaite PA, Tuder RM, Erzurum SC, Geraci MW, Coldren CD 
In:
Am J Respir Crit Care Med (2010) 182(9): 1153-60 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Lu X, Murphy TC, Nanes MS, Hart CM 
In:
Am J Physiol Lung Cell Mol Physiol (2010) 299(4): L599-66 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Davis BN, Hilyard AC, Nguyen PH, Lagna G, Hata A 
In:
Mol Cell (2010) 39(3): 373-84 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Chan MC, Hilyard AC, Wu C, Davis BN, Hill NS, Lal A, Lieberman J, Lagna G, Hata A 
In:
EMBO J (2010) 29(3): 559-73 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Chan SY, Zhang YY, Hemann C, Mahoney CE, Zweier JL, Loscalzo J 
In:
Cell Metab (2009) 10(4): 273-84 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Yu Y, Keller SH, Remillard CV, Safrina O, Nicholson A, Zhang SL, Jiang W, Vangala N, Landsberg JW, Wang JY, Thistlethwaite PA, Channick RN, Robbins IM, Loyd JE, Ghofrani HA, Grimminger F, Schermuly RT, Cahalan MD, Rubin LJ, Yuan JX 
In:
Circulation (2009) 19(17): 2313-22 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Davis BN, Hilyard AC, Lagna G, Hata A 
In:
Nature (2008) 454(7200): 56-61 
Categories:
Primary Cells and Media 
Authors:
Belaiba RS, Bonello S, Zähringer C, Schmidt S, Hess J, Kietzmann T, Görlach A 
In:
Mol Biol Cell (2007) 18(12): 4691-7 
Categories:
Transfection 
Authors:
Yang X, Lee PJ, Long L, Trembath RC, Morrell NW 
In:
Am J Respir Cell Mol Biol (2007) 37(5): 598-605 
Categories:
Transfection 
Authors:
Zakrzewicz A, Hecker M, Marsh LM, Kwapiszewska G, Nejman B, Long L, Seeger W, Schermuly RT, Morrell NW, Morty RE, Eickelberg O 
In:
Circulation (2007) 115(23): 2957-68 
Categories:
Transfection 
Authors:
Sturrock A, Cahill B, Norman K, Huecksteadt TP, Hill K, Sanders K, Karwande SV, Stringham JC, Bull DA, Gleich M, Kennedy TP and Hoidal JR 
In:
Am J Physiol Lung Cell Mol Physiol (2006) 290(4): L661-73 
Categories:
Transfection 
Authors:
Yang X, Long L, Southwood M, Rudarakanchana N, Upton PD, Jeffery TK, Atkinson C, Chen H, Trembath RC and Morrell NW 
In:
Circ Res (2005) 96(10): 1053-1063 
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