SMC, pul.artery (PASMC), human

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Primary SMC isolated from human pulmonary artery

Cell Type:
Smooth muscle cell
Tissue Origin:
artery
Species:
human
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Research Area:
Cardiovascular
Cell Characteristics:
Adherent

Recommended Media

Smooth Muscle Growth Medium-2

 SmGM-2 was developed for the maintenance and expansion of human smooth muscle cells. This medium contains 5% FBS.

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
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The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
Smooth muscle, basic A-033 2e6 90% 85% Plasmid (general) 2 µg 100 µl I/II/2b
P1

FG-113

 1e5 85% 55% Plasmid (general) 0.2 µg 20 µl 4D X-Unit
P1 FG-113 1e5 85% 55% Plasmid (general) 0.2 µg 20 µl 96-well

Citations (27)

An organ-on-chip model of pulmonary arterial hypertension identifies a BMPR2-SOX17-prostacyclin signaling axis
Categories:
Primary Cells and Media, 3D Cell Culture  
Authors:
Alexander J Ainscough , Timothy J Smith , Maike Haensel , Christopher J Rhodes , Adam Fellows , Harry J Whitwell , Eleni Vasilaki , Kelly Gray, Adrian Freeman , Luke S Howard , John Wharton , Benjamin Dunmore , Paul D Upton , Martin R Wilkins , Joshua B Edel, Beata Wojciak-Stothard  
In:
Nat Commun. (2022) 5: 1192 
Mesenchymal Stem Cells Conditioned Media Suppress Inflammation Associated Over-proliferation of Pulmonary Artery Smooth Muscle Cells in a Rat Model of Pulmonary Hypertension
Categories:
Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Liu JF, Han Z, Han ZC, HeZX 
In:
Other (2014) 20(8): 3411-3439 
Recombinant human interferon alpha 2b prevents and reverses experimental pulmonary hypertension.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Bauer EM, Zheng H, Lotze MT, Bauer PM. 
In:
PLoS ONE (2014) 9(5): e96720 
Tumor stiffness is unrelated to myosin light chain phosphorylation in cancer cells
Categories:
Primary Cells and Media 
Authors:
Yu HJ1, Serebryannyy LA, Fry M, Greene M, Chernaya O, Hu WY, Chew TL, Mahmud N, Kadkol SS, Glover S, Prins G, Strakova Z, de Lanerolle P 
In:
PLoS ONE (2013) 8(11): e79776 
Evaluating extracellular matrix influence on adherent cell signaling by cold trypsin phosphorylation-specific flow cytometry
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Abrahamsen I, Lorens JB 
In:
BMC Cell Biol (2013) 14:36: 1-7 
Serum can overcome contact inhibition in confluent human pulmonary artery smooth muscle cells
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Solodushko V, Khader HA, Fouty BW 
In:
PLoS ONE (2013) 8(8): e71490 
PPARd agonist GW501516 inhibits PDGF-stimulated pulmonary arterial smooth muscle cell function related to pathological vascular remodeling
Categories:
Primary Cells and Media 
Authors:
Liu G, Li X, Li Y, Tang X, Xu J, Li R, Hao P, Sun Y. 
In:
Biomed Res Int. (2013) 2013: 903947 
Pharmacologic agents elevating cAMP prevent arginase II expression and proliferation of pulmonary artery smooth muscle cells
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Chen B, Calvert AE, Meng X, Nelin LD 
In:
Am J Respir Cell Mol Biol (2012) 47(2): 218-26 
Hypoxia-induced pulmonary arterial smooth muscle cell proliferation is controlled by forkhead box M1
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Raghavan A, Zhou G, Zhou Q, Ibe JC, Ramchandran R, Yang Q, Racherla H, Raychaudhuri P, Raj JU 
In:
Am J Respir Cell Mol Biol (2012) 46(4): 431-6 
Altered MicroRNA processing in heritable pulmonary arterial hypertension: an important role for Smad-8
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Drake KM, Zygmunt D, Mavrakis L, Harbor P, Wang L, Comhair SA, Erzurum SC, Aldred MA 
In:
Am J Respir Crit Care Med (2011) 184(12): 1400-8 
Hypoxia potentiates microRNA-mediated gene silencing through posttranslational modification of Argonaute2
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Wu C, So J, Davis-Dusenbery BN, Qi HH, Bloch DB, Shi Y, Lagna G, Hata A 
In:
Mol Cell Biol (2011) 31(23): 4760-74 
Xanthine oxidase-derived ROS upregulate Egr-1 via ERK1/2 in PA smooth muscle cells; model to test impact of extracellular ROS in chronic hypoxia.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Hartney T, Birari R, Venkataraman S, Villegas L, Martinez M, Black SM, Stenmark KR, Nozik-Grayck E. 
In:
PLoS ONE (2011) 6(11): e27531 
BMP promotes motility and represses growth of smooth muscle cells by activation of tandem Wnt pathways
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media, Transfection 
Authors:
Perez VA, Ali Z, Alastalo TP, Ikeno F, Sawada H, Lai YJ, Kleisli T, Spiekerkoetter E, Qu X, Rubinos LH, Ashley E, Amieva M, Dedhar S, Rabinovitch M 
In:
J Cell Biol (2011) 192(1): 171-88 
Smooth muscle proliferation and role of the prostacyclin (IP) receptor in idiopathic pulmonary arterial hypertension
Categories:
Primary Cells and Media 
Authors:
Falcetti E, Hall SM, Phillips PG, Patel J, Morrell NW, Haworth SG, Clapp LH 
In:
Am J Respir Crit Care Med (2010) 182(9): 1161-70 
Somatic chromosome abnormalities in the lungs of patients with pulmonary arterial hypertension
Categories:
Primary Cells and Media 
Authors:
Aldred MA, Comhair SA, Varella-Garcia M, Asosingh K, Xu W, Noon GP, Thistlethwaite PA, Tuder RM, Erzurum SC, Geraci MW, Coldren CD 
In:
Am J Respir Crit Care Med (2010) 182(9): 1153-60 
PPAR regulates hypoxia-induced Nox4 expression in human pulmonary artery smooth muscle cells through NF-B.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Lu X, Murphy TC, Nanes MS, Hart CM 
In:
Am J Physiol Lung Cell Mol Physiol (2010) 299(4): L599-66 
Endothelin-1 (ET-1) increases the expression of remodeling genes in vascular smooth muscle through linked calcium and cAMP pathways: role of a phospholipase A(2)(cPLA(2))/cyclooxygenase-2 (COX-2)/prostacyclin receptor-dependent autocrine loop
Categories:
Primary Cells and Media 
Authors:
Deacon K, Knox AJ. 
In:
J Biol Chem (2010) 285(34): 25913-27 
Smad proteins bind a conserved RNA sequence to promote microRNA maturation by Drosha
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Davis BN, Hilyard AC, Nguyen PH, Lagna G, Hata A 
In:
Mol Cell (2010) 39(3): 373-84 
Molecular basis for antagonism between PDGF and the TGFbeta family of signalling pathways by control of miR-24 expression
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Chan MC, Hilyard AC, Wu C, Davis BN, Hill NS, Lal A, Lieberman J, Lagna G, Hata A 
In:
EMBO J (2010) 29(3): 559-73 
MicroRNA-210 controls mitochondrial metabolism during hypoxia by repressing the iron-sulfur cluster assembly proteins ISCU1/2
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Chan SY, Zhang YY, Hemann C, Mahoney CE, Zweier JL, Loscalzo J 
In:
Cell Metab (2009) 10(4): 273-84 
A functional single-nucleotide polymorphism in the TRPC6 gene promoter associated with idiopathic pulmonary arterial hypertension
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Yu Y, Keller SH, Remillard CV, Safrina O, Nicholson A, Zhang SL, Jiang W, Vangala N, Landsberg JW, Wang JY, Thistlethwaite PA, Channick RN, Robbins IM, Loyd JE, Ghofrani HA, Grimminger F, Schermuly RT, Cahalan MD, Rubin LJ, Yuan JX 
In:
Circulation (2009) 19(17): 2313-22 
SMAD proteins control DROSHA-mediated microRNA maturation
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Davis BN, Hilyard AC, Lagna G, Hata A 
In:
Nature (2008) 454(7200): 56-61 
Hypoxia up-regulates hypoxia-inducible factor-1alpha transcription by involving phosphatidylinositol 3-kinase and nuclear factor kappaB in pulmonary artery smooth muscle cells
Categories:
Primary Cells and Media 
Authors:
Belaiba RS, Bonello S, Zähringer C, Schmidt S, Hess J, Kietzmann T, Görlach A 
In:
Mol Biol Cell (2007) 18(12): 4691-7 
BMP4 Induces HO-1 via a Smad-Independent, p38MAPK-Dependent Pathway in Pulmonary Artery Myocytes
Categories:
Transfection 
Authors:
Yang X, Lee PJ, Long L, Trembath RC, Morrell NW 
In:
Am J Respir Cell Mol Biol (2007) 37(5): 598-605 
Receptor for Activated C-Kinase 1, a Novel Interaction Partner of Type II Bone Morphogenetic Protein Receptor, Regulates Smooth Muscle Cell Proliferation in Pulmonary Arterial Hypertension
Categories:
Transfection 
Authors:
Zakrzewicz A, Hecker M, Marsh LM, Kwapiszewska G, Nejman B, Long L, Seeger W, Schermuly RT, Morrell NW, Morty RE, Eickelberg O 
In:
Circulation (2007) 115(23): 2957-68 
Transforming Growth Factor 1 Induces Nox 4 NAD(P)H Oxidase and Reactive Oxygen Species-Dependent Proliferation in Human Pulmonary Artery Smooth Muscle Cells
Categories:
Transfection 
Authors:
Sturrock A, Cahill B, Norman K, Huecksteadt TP, Hill K, Sanders K, Karwande SV, Stringham JC, Bull DA, Gleich M, Kennedy TP and Hoidal JR 
In:
Am J Physiol Lung Cell Mol Physiol (2006) 290(4): L661-73 
Dysfunctional Smad signaling contributes to abnormal smooth muscle cell proliferation in familial pulmonary arterial hypertension
Categories:
Transfection 
Authors:
Yang X, Long L, Southwood M, Rudarakanchana N, Upton PD, Jeffery TK, Atkinson C, Chen H, Trembath RC and Morrell NW 
In:
Circ Res (2005) 96(10): 1053-1063 
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