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Burkitt's lymphoma, B lymphocyte

Cell Type:
B cell
Tissue Origin:
Species:
human
Research Area:
Cancer Research/Cell Biology
Immunotherapy / Hematology
Cell Characteristics:
Suspension

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
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The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
SG DS-104 4e5 54-76% 66-76% Plasmid (general) 400 ng 20 µl 4D X-Unit
SG DS-104 2e6 63-75% 53-89% Plasmid (general) 2 µg 100 µl 4D X-Unit
V M-013 2e6 52-64% 61-73% Plasmid (general) 2 µg 100 µl I/II/2b

Citations (15)

Abrogation of HLA surface expression using CRISPR/Cas9 genome editing: a step toward universal T cell therapy
Categories:
Transfection 
Authors:
Lee J, Sheen JH, Lim O, Lee Y, Ryu J, Shin D, Kim YY, Kim M. 
In:
Scientific Reports (2020) 20;10(1): :17753 
T Cells Expressing CD19/CD20 Bispecific Chimeric Antigen Receptors Prevent Antigen Escape by Malignant B Cells.
Categories:
Transfection 
Authors:
Zah E, Lin MY, Silva-Benedict A, Jensen MC, Chen YY 
In:
Other (2016) 4(6): 498-508 
RNA-guided endonuclease provides a therapeutic strategy to cure latent herpesviridae infection
Categories:
Transfection 
Authors:
Wang J, Quake SR 
In:
Proc Natl Acad Sci USA (2014) 111(36): 13157-62 
STAP-2 negatively regulates both canonical and non-canonical NF-kappaB activation induced by Epstein-Barr virus-derived LMP1
Categories:
Transfection 
Authors:
Ikeda O, Sekine Y, Yasui T, Oritani K, Sugiyma K, Muromoto R, Ohbayashi N, Yoshimura A, Matsuda T 
In:
Mol Cell Biol (2008) 28(16): 5027-42 
The insulator factor CTCF controls MHC class II gene expression and is required for the formation of long-distance chromatin interactions
Categories:
Transfection 
Authors:
Majumder P, Gomez JA, Chadwick BP, Boss JM 
In:
J Exp Med (2008) 205(4): 785-98 
In Vivo and in Vitro Intragenomic Rearrangement of TT Viruses
Categories:
Transfection 
Authors:
Leppik L, Gunst K, Lehtinen M, Dillner J, Streker K, de Villiers EM 
In:
J Virol (2007) 81(17): 9346-56 
Gene expression analysis identifies novel RBL2/p130 target genes in endemic Burkitt's lymphoma cell lines and primary tumors
Categories:
Transfection 
Authors:
De Falco G, Leucci E, Lenze D, Piccaluga PP, Claudio PP, Onnis A, Cerino G, Nyagol J, Mwanda W, Bellan C, Hummel M, Pileri S, Tosi P, Stein H, Giordano A, Leoncini L 
In:
Blood (2007) 110(4): 1301-7 
Inhibition of BCL11B expression leads to apoptosis of malignant but not normal mature T cells
Categories:
Transfection 
Authors:
Grabarczyk P, Przybylski GK, Depke M, Volker U, Bahr J, Assmus K, Broker BM, Walther R, Schmidt CA 
In:
Oncogene (2007) 26(26): 3797-810 
Mcl-1 is a relevant therapeutic target in acute and chronic lymphoid malignancies: down-regulation enhances rituximab-mediated apoptosis and complement-dependent cytotoxicity
Categories:
Transfection 
Authors:
Hussain SR, Cheney CM, Johnson AJ, Lin TS, Grever MR, Caligiuri MA, Lucas DM, Byrd JC 
In:
Clin Cancer Res (2007) 13(7): 2144-50 
Protein Kinase C Is Essential to Maintain CIITA Gene Expression in B Cells
Categories:
Transfection 
Authors:
Kwon MJ, Soh JW, Chang CH 
In:
J Immunol (2006) 177(2): 950-6 
Interferon-inducible protein IFIXalpha1 functions as a negative regulator of HDM2
Categories:
Transfection 
Authors:
Ding Y, Lee JF, Lu H, Lee MH, Yan DH 
In:
Mol Cell Biol (2006) 26(5): 1979-96 
Suppression of Epstein-Barr nuclear antigen 1 (EBNA1) by RNA interference inhibits proliferation of EBV-positive Burkitt's lymphoma cells
Categories:
Transfection 
Authors:
Hong M, Murai Y, Kutsuna T, Takahashi H, Nomoto K, Cheng CM, Ishizawa S, Zhao QL, Ogawa R, Harmon BV, Tsuneyama K, Takano Y 
In:
J Cancer Res Clin Oncol (2006) 132(1): 1-8 
Mutation of a Single Amino Acid Residue in the Basic Region of the Epstein-Barr Virus (EBV) Lytic Cycle Switch Protein Zta (BZLF1) Prevents Reactivation of EBV from Latency
Categories:
Transfection 
Authors:
Schelcher C, Valencia S, Delecluse HJ, Hicks M and Sinclair AJ 
In:
J Virol (2005) 79(21): 13822-13828 
EphrinB2 is the entry receptor for Nipah virus, an emergent deadly paramyxovirus
Categories:
Transfection 
Authors:
Negrete OA, Levroney EL, Aguilar HC, Bertolotti-Ciarlet A, Nazarian R, Tajyar S and Lee B 
In:
Nature (2005) 436(7049): 401-405 
CUL1, a component of E3 ubiquitin ligase, alters lymphocyte signal transduction with possible effect on rheumatoid arthritis
Categories:
Transfection 
Authors:
Kawaida R, Yamada R, Kobayashi K, Tokuhiro S, Suzuki A, Kochi Y, Chang X, Sekine A, Tsunoda T, Sawada T, Furukawa H, Nakamura Y and Yamamoto K 
In:
Genes Immun (2005) 6(3): 194-202 
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