C2C12
Export
myoblast cell line, with fibroblast-like morphology
Cell Type:
Myoblast
Tissue Origin:
skeletal muscle
Species:
mouse
Cell Characteristics:
Adherent
Transfection Information
Lonza Optimized Protocol
Optimization Guideline
Filter:
Any Substrate
Any Platform
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols
or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our
optimization strategy
to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol
Kit
Program
Cells
Efficiency
Viable Cells
Substrate
Format
Platform
SE
CD-137
1e6
74-76%
73%
Plasmid (general)
2 µg
100 µl
4D X-Unit
SE
CD-137
2e5
79-81%
70%
Plasmid (general)
400 ng
20 µl
4D X-Unit
V
B-032
1e6
76-88%
90-96%
Plasmid (general)
2 µg
100 µl
I/II/2b
AD2
FB-166
1e4
41-49%
55-71%
Plasmid (general)
16 µg
350 µl
4D Y-Unit
AD2
FB-166
1e4
49-51%
88-96%
Plasmid (general)
16 µg
350 µl
4D Y-Unit
Citations (9)
Inhibition of Rac1 promotes BMP-2-induced osteoblastic differentiation.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media
Authors:
Onishi M, Fujita Y, Yoshikawa H, Yamashita T
In:
Cell Death Dis. (2013) 4: e698
ATF-1 is a hypoxia-responsive transcriptional activator of skeletal muscle uncoupling protein 3
Categories:
Transfection
Authors:
Lu Z, Sack MN
In:
J Biol Chem (2008) epub: online
RNA toxicity in myotonic muscular dystrophy induces NKX2-5 expression
Categories:
Transfection
Authors:
Yadava RS, Frenzel-McCardell CD, Yu Q, Srinivasan V, Tucker AL, Puymirat J, Thornton CA, Prall OW, Harvey RP, Mahadevan MS
In:
Nat Genet (2008) 40(1): 61-8
Beta-catenin regulates acetylcholine receptor clustering in muscle cells through interaction with rapsyn
Categories:
Transfection
Authors:
Zhang B, Luo S, Dong XP, Zhang X, Liu C, Luo Z, Xiong WC, Mei L
In:
J Neurosci (2007) 27(15): 3968-73
Inositide-dependent phospholipase C signaling mimics insulin in skeletal muscle differentiation by affecting specific regions of the cyclin D3 promoter
Categories:
Transfection
Authors:
Faenza I, Ramazzotti G, Bavelloni A, Fiume R, Gaboardi GC, Follo MY, Gilmour RS, Martelli AM, Ravid K, Cocco L
In:
Endocrinology (2007) 148(3): 1108-17
C6ORF32 is upregulated during muscle cell differentiation and induces the formation of cellular filopodia
Categories:
Transfection
Authors:
Yoon S, Molloy MJ, Wu MP, Cowan DB, Gussoni E
In:
Dev Biol (2007) 301(1): 70-81
Gene therapy with an improved doxycycline regulated plasmid encoding a TNFalpha inhibitor in experimental arthritis
Categories:
Transfection
Authors:
Gould DJ, Yousaf N, Fatah R, Subang MC, Chernajovsky Y
In:
Arthritis Res Ther (2007) 9(1): R7
Identification of potential target genes for RFX4_v3, a transcription factor critical for brain development
Categories:
Transfection
Authors:
Zhang D, Stumpo DJ, Graves JP, Degraff LM, Grissom SF, Collins JB, Li L, Zeldin DC, Blackshear PJ
In:
J Neurochem (2006) 98(3): 860-75
Amino acid deprivation induces both apoptosis and autophagy in murine C2C12 muscle cells
Categories:
Transfection
Authors:
Martinet W, De Meyer GR, Herman AG, Kockx MM
In:
Biotechnol Lett (2005) 27(16): 1157-63
+ Show All