cells

myoblast cell line, with fibroblast-like morphology

Cell Type:

Myoblast

Tissue Origin:

skeletal muscle

Species:

mouse

Cell Characteristics:

Adherent

Transfection Information

Lonza Optimized Protocol
Optimization Guideline

Filter:

The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.

Kit	Program	Cells	Efficiency	Viable Cells	Substrate		Format	Platform
SE	CD-137	1e6	74-76%	73%	Plasmid (general)	2 µg	100 µl	4D X-Unit
SE	CD-137	2e5	79-81%	70%	Plasmid (general)	400 ng	20 µl	4D X-Unit
V	B-032	1e6	76-88%	90-96%	Plasmid (general)	2 µg	100 µl	I/II/2b
AD2	FB-166	1e4	41-49%	55-71%	Plasmid (general)	16 µg	350 µl	4D Y-Unit
AD2	FB-166	1e4	49-51%	88-96%	Plasmid (general)	16 µg	350 µl	4D Y-Unit

Citations (10)

Crabtree effect in kidney proximal tubule cells via late-stage glycolytic intermediates

Categories:

Primary Cells and Media, Bioassay

Authors:

Manjula Darshi , Jana Tumova , Afaf Saliba , Jiwan Kim , Judy Baek , Subramaniam Pennathur , Kumar Sharma

In:

iScience (2023) 26(4) : 1-14

Inhibition of Rac1 promotes BMP-2-induced osteoblastic differentiation.

Categories:

Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media

Authors:

Onishi M, Fujita Y, Yoshikawa H, Yamashita T

In:

Cell Death Dis. (2013) 4: e698

ATF-1 is a hypoxia-responsive transcriptional activator of skeletal muscle uncoupling protein 3

Categories:

Transfection

Authors:

Lu Z, Sack MN

In:

J Biol Chem (2008) epub: online

RNA toxicity in myotonic muscular dystrophy induces NKX2-5 expression

Categories:

Transfection

Authors:

Yadava RS, Frenzel-McCardell CD, Yu Q, Srinivasan V, Tucker AL, Puymirat J, Thornton CA, Prall OW, Harvey RP, Mahadevan MS

In:

Nat Genet (2008) 40(1): 61-8

Beta-catenin regulates acetylcholine receptor clustering in muscle cells through interaction with rapsyn

Categories:

Transfection

Authors:

Zhang B, Luo S, Dong XP, Zhang X, Liu C, Luo Z, Xiong WC, Mei L

In:

J Neurosci (2007) 27(15): 3968-73

Inositide-dependent phospholipase C signaling mimics insulin in skeletal muscle differentiation by affecting specific regions of the cyclin D3 promoter

Categories:

Transfection

Authors:

Faenza I, Ramazzotti G, Bavelloni A, Fiume R, Gaboardi GC, Follo MY, Gilmour RS, Martelli AM, Ravid K, Cocco L

In:

Endocrinology (2007) 148(3): 1108-17

C6ORF32 is upregulated during muscle cell differentiation and induces the formation of cellular filopodia

Categories:

Transfection

Authors:

Yoon S, Molloy MJ, Wu MP, Cowan DB, Gussoni E

In:

Dev Biol (2007) 301(1): 70-81

Gene therapy with an improved doxycycline regulated plasmid encoding a TNFalpha inhibitor in experimental arthritis

Categories:

Transfection

Authors:

Gould DJ, Yousaf N, Fatah R, Subang MC, Chernajovsky Y

In:

Arthritis Res Ther (2007) 9(1): R7

Identification of potential target genes for RFX4_v3, a transcription factor critical for brain development

Categories:

Transfection

Authors:

Zhang D, Stumpo DJ, Graves JP, Degraff LM, Grissom SF, Collins JB, Li L, Zeldin DC, Blackshear PJ

In:

J Neurochem (2006) 98(3): 860-75

Amino acid deprivation induces both apoptosis and autophagy in murine C2C12 muscle cells

Categories:

Transfection

Authors:

Martinet W, De Meyer GR, Herman AG, Kockx MM

In:

Biotechnol Lett (2005) 27(16): 1157-63

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