A549

Human lung carcinoma established from an explanted lung tumor which was removed from a 58-year-old Caucasian man (Giard DJ , et al. J. Natl. Cancer Inst. (1973) 51: 1417-1423).

Cell Type:
Respiratory Tract Epith.
Tissue Origin:
lung
Species:
human
Research Area:
Cancer Research/Cell Biology
Dermatology/Tissue Engineering
Cell Characteristics:
Adherent

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
SF CM-130 2e5 79-81% 56-64% Plasmid (general) 200 ng 20 µl 4D X-Unit
SF CM-130 1e6 79-83% 60-66% Plasmid (general) 1 µg 100 µl 4D X-Unit
T X-001 1e6 69-75% 74-88% Plasmid (general) 2 µg 100 µl I/II/2b

Citations (12)

Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Powell JD, Hutchison JR, Hess BM, Straub TM 
In:
Other (2015) 119(3): 711-23 
Categories:
Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Jujo T, Sakao S, Tsukahara M, Kantake S, Maruoka M, Tanabe N, Masuda M, Tatsumi K. 
In:
PLoS ONE (2014) 9(1): 1371 
Categories:
Transfection 
Authors:
Grzesik BA, Vohwinkel CU, Morty RE, Mayer K, Herold S, Seeger W, Vadász I. 
In:
Am J Physiol Lung Cell Mol Physiol (2013) epub: 103(9) 
Categories:
Transfection 
Authors:
Schmeck B, Lorenz J, N'guessan PD, Opitz B, van Laak V, Zahlten J, Slevogt H, Witzenrath M, Flieger A, Suttorp N, Hippenstiel S 
In:
J Immunol (2008) 181(2): 940-7 
Categories:
Transfection 
Authors:
Jaitovich A, Mehta S, Na N, Ciechanover A, Goldman RD, Ridge KM 
In:
J Biol Chem (2008) epub: online 
Categories:
Transfection 
Authors:
Vinzing M, Eitel J, Lippmann J, Hocke AC, Zahlten J, Slevogt H, N'guessan PD, Günther S, Schmeck B, Hippenstiel S, Flieger A, Suttorp N, Opitz B 
In:
J Immunol (2008) 180(10): 6808-15 
Categories:
Transfection 
Authors:
Iles N, Rulten S, El-Khamisy SF, Caldecott KW 
In:
Mol Cell Biol (2007) 27(10): 3793-803 
Categories:
Transfection 
Authors:
Opitz B, Vinzing M, van Laak V, Schmeck B, Heine G, Gunther S, Preissner R, Slevogt H, N Guessan PD, Eitel J, Goldmann T, Flieger A, Suttorp N, Hippenstiel S 
In:
J Biol Chem (2006) 281(47): 36173-9 
Categories:
Transfection 
Authors:
Martinez-Sobrido L, Zuniga EI, Rosario D, Garcia-Sastre A, de la Torre JC 
In:
J Virol (2006) 80(18): 9192-9 
Categories:
Transfection 
Authors:
Wooten LG and Ogretmen B 
In:
J Biol Chem (2005) 280(32): 28867-28876 
Categories:
Transfection 
Authors:
Woo HA, Jeong W, Chang TS, Park KJ, Park SJ, Yang JS and Rhee SG 
In:
J Biol Chem (2005) 280(5): 3125-3128 
Categories:
Transfection 
Authors:
Chang TS, Jeong W, Woo HA, Lee SM, Park S and Rhee SG 
In:
J Biol Chem (2004) 279(49): 50994-51001 
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