CCRF-CEM

acute lymphoblastic leukemia cell line, T-lymphoblast

Cell Type:
T Cell
Tissue Origin:
blood
Species:
human
Research Area:
Cancer Research/Cell Biology
Immunotherapy / Hematology
Cell Characteristics:
Suspension

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
SF DC-100 4e5 50% ±7 48% ±14 Plasmid (general) 0.4 µg 20 µl 4D X-Unit
SF DC-100 2e6 66% ±8 21% ±10 Plasmid (general) 2 µg 100 µl 4D X-Unit
C X-001 2e6 66-70% 75-82% Plasmid (general) 2 µg 100 µl I/II/2b

Citations

Categories:
Transfection 
Authors:
Leclerc GJ, Leclerc GM, Kinser TT, Barredo JC 
In:
BMC Cancer (2006) 6: 132 
Categories:
Transfection 
Authors:
Tiwari S, Dong H, Kim EJ, Weintraub L, Epstein PM and Lerner A 
In:
Biochem Pharmacol (2005) 69(3): 473-483