CCRF-CEM
Export
acute lymphoblastic leukemia cell line, T-lymphoblast
Cell Type:
T Cell
Tissue Origin:
blood
Species:
human
Research Area:
Cancer Research/Cell Biology
Immunotherapy / Hematology
Cell Characteristics:
Suspension
Transfection Information
Lonza Optimized Protocol
Optimization Guideline
Filter:
Any Substrate
Any Platform
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols
or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our
optimization strategy
to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol
Kit
Program
Cells
Efficiency
Viable Cells
Substrate
Format
Platform
SF
DC-100
4e5
50% ±7
48% ±14
Plasmid (general)
0.4 µg
20 µl
4D X-Unit
SF
DC-100
2e6
66% ±8
21% ±10
Plasmid (general)
2 µg
100 µl
4D X-Unit
C
X-001
2e6
66-70%
75-82%
Plasmid (general)
2 µg
100 µl
I/II/2b
Citations
Analysis of folylpoly-gamma-glutamate synthetase gene expression in human B-precursor ALL and T-lineage ALL cells
Categories:
Transfection
Authors:
Leclerc GJ, Leclerc GM, Kinser TT, Barredo JC
In:
BMC Cancer (2006) 6: 132
Type 4 cAMP phosphodiesterase (PDE4) inhibitors augment glucocorticoid-mediated apoptosis in B cell chronic lymphocytic leukemia (B-CLL) in the absence of exogenous adenylyl cyclase stimulation
Categories:
Transfection
Authors:
Tiwari S, Dong H, Kim EJ, Weintraub L, Epstein PM and Lerner A
In:
Biochem Pharmacol (2005) 69(3): 473-483