Keratinocyte, (NHEK-neo) human neonatal

Primary keratinocyte.

Keratinocytes derived from human neonatal foreskins.

Cell Type:
Skin/Hair Epith.
Tissue Origin:
dermal
Species:
human
Research Area:
Cancer Research/Cell Biology
Dermatology/Tissue Engineering
Cell Characteristics:
Suspension

Recommended Media

Our first media specialised for growing keratinocytes.
Now superceded by KGM-Gold

The original medium for growth of keratinocytes but without Calcium.

Supports proliferation of primary human keratinocytes in culture.
Drives faster proliferation than the original KGM.
Now superceded by KGM-Gold

A serum-free, calcium free keratinocyte growth medium

Optimized for Normal Human Epidermal Keratinocytes in a serum-free environment.
Provides a nutrient rich medium that provides normal physiological growth characteristics and population doubling times.
KGM-Gold supports low, moderate, and high density keratinocyte cultures better than KGM or KGM-2, without nutrient depletion.

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
P3

96-DS-138

1e5 68% 53-61% Plasmid (general) 0.4 µg 20 µl Shuttle
P3

96-DS-138

1e5 78% 73-85% Plasmid (general) 0.4 µg 20 µl Shuttle
Keratinocyte, human T-018 5e5 53% ±13 50-60% Plasmid (general) 2.5 µg 100 µl I/II/2b
P3

DS-138

1e5 63% 64-70% Plasmid (general) 0.4 µg 20 µl 4D X-Unit
P3

DS-138

5e5 59% 70-76% Plasmid (general) 2 µg 100 µl 4D X-Unit

Citations (23)

Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
von Lanzenauer SH, Wolk K, Höflich C, Kunz S, Grünberg BH, Döcke WD, Reineke U, Asadullah K, Sterry W, Volk HD, Sabat R 
In:
Genes Immun (2015) 16(1): ePub 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Ramaswamy K, Kumar P, He YX. 
In:
J Immunol (2014) 165(8): 4567-74 
Categories:
Primary Cells and Media 
Authors:
Vidaurre S1, Fitzpatrick C2, Burzio VA3, Briones M2, Villota C2, Villegas J2, Echenique J2, Oliveira-Cruz L2, Araya M2, Borgna V2, Socías T2, Lopez C2, Avila R2, Burzio LO2 
In:
J Biol Chem (2014) M114: 558841 
Categories:
Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Jeong MS, Kim JY, Lee HI, Seo SJ. 
In:
Ann Dermatol (2014) 26(3): 296-302 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Gutowska-Owsiak D, Salimi M, Selvakumar TA, Wang X, Taylor S, Ogg GS. 
In:
J Investig Allergol Clin Immunol (2014) 24(4): 231-9 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Kim HJ, Chang H, Han SH, Lee MS, Jung JY, An S, Baek SY, Lee JH, Lee JH, Lee TR, Shin DW, Kim H 
In:
Int J Mol Sci (2014) 15(4): 5749-61 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Yoshida S1, Lee JO2, Nakamura K1, Suzuki S3, Hendon DN2, Kobayashi M4, Suzuki F4. 
In:
PLoS ONE (2014) 9(2): e82926 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Stahley SN, Saito M, Faundez V, Koval M, Mattheyses AL, Kowalczyk AP 
In:
PLoS ONE (2014) 9(1): e87809 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Barr TP1, Albrecht PJ, Hou Q, Mongin AA, Strichartz GR, Rice FL. 
In:
PLoS ONE (2013) 8(2): e56744 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Lamb R1, Ambler CA. 
In:
PLoS ONE (2013) 8(1): e52494 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Xu K1, Fu Y, Chung W, Zheng X, Cui Y, Hsu IC, Kao WJ. 
In:
Acta Biomaterialia (2012) 8(7): 2504-16 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Uchino T, Takezawa T, Ikarashi Y, Nishimura T 
In:
Other (2011) 16(1): 1-8 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Panopoulos AD, Ruiz S, Yi F, Herrerías A, Batchelder EM, Izpisua Belmonte JC. 
In:
PLoS ONE (2011) 6(5): e19743 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Chang KC1, Wang Y, Oh IG, Jenkins S, Freedman LP, Thompson CC, Chung JH, Nagpal S. 
In:
Mol Pharmacol (2010) 77(5): 744-50 
Categories:
Primary Cells and Media 
Authors:
Tengara S, Tominaga M, Kamo A, Taneda K, Negi O, Ogawa H, Takamori K. 
In:
J Dermatol Sci (2010) 58(1):: 64-71 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Samberg ME, Oldenburg SJ, Monteiro-Riviere NA. 
In:
Environ Health Perspect (2010) 118(3): 407-13 
Categories:
Transfection 
Authors:
Allen D, Winters E, Kenna PF, Humphries P, Jane Farrar G 
In:
J Dermatol Sci (2008) 49(3): 217-25 
Categories:
Transfection 
Authors:
Mangold U, Hayakawa H, Coughlin M, Münger K, Zetter BR 
In:
Oncogene (2008) 27(5): 604-13 
Categories:
Transfection 
Authors:
Xue M, Campbell D, Jackson CJ 
In:
J Biol Chem (2007) 282(18): 13610-6 
Categories:
Transfection 
Authors:
Truong AB, Kretz M, Ridky TW, Kimmel R, Khavari PA 
In:
Genes Dev (2006) 20(22): 3185-97 
Categories:
Transfection 
Authors:
Xu Y, Shao Y, Voorhees JJ, Fisher GJ 
In:
J Biol Chem (2006) 281(37): 27389-97 
Categories:
Transfection 
Authors:
Harada K, Truong AB, Cai T and Khavari PA 
In:
Mol Cell Biol (2005) 25(24): 11122-11130 
Categories:
Transfection 
Authors:
Gresch O, Engel FB, Nesic D, Tran TT, England HM, Hickman ES, Korner I, Gan L, Chen S, Castro-Obregon S, Hammermann R, Wolf J, Muller-Hartmann H, Nix M, Siebenkotten G, Kraus G and Lun K 
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