cells

spontaneously transformed human keratinocyte cell line

Cell Type:

Skin/Hair Epith.

Tissue Origin:

dermal

Species:

human

Research Area:

Cancer Research/Cell Biology

Dermatology/Tissue Engineering

Cell Characteristics:

Adherent

Transfection Information

Lonza Optimized Protocol
Optimization Guideline

Filter:

The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.

Protocol	Kit	Program	Cells	Efficiency	Viable Cells	Substrate		Format	Platform
	V	U-020	1e6	31-55%		Plasmid (general)	2 µg	100 µl	I/II/2b

Citations (12)

Inhibition of Phosphatidylcholine-Specific Phospholipase C Interferes with Proliferation and Survival of Tumor Initiating Cells in Squamous Cell Carcinoma

Categories:

Primary Cells and Media, Classical Media and Reagents

Authors:

Cecchetti S, Bortolomai I, Ferri R, Mercurio L, Canevari S, Podo F, Miotti S, Iorio E.

In:

PLoS ONE (2015) 10(9): e0136120

UVA-induced cell cycle progression is mediated by a disintegrin and metalloprotease/epidermal growth factor receptor/AKT/Cyclin D1 pathways in keratinocytes

Categories:

Transfection

Authors:

He YY, Council SE, Feng L, Chignell CF

In:

Cancer Res (2008) 68(10): 3752-8

Differential expression of D-type cyclins in HaCaT keratinocytes and in psoriasis

Categories:

Transfection

Authors:

Belso N, Szell M, Pivarcsi A, Kis K, Kormos B, Kenderessy AS, Dobozy A, Kemeny L, Bata-Csorgo Z

In:

J Invest Dermatol (2008) 128(3): 634-42

Spatial distribution of protein damage by singlet oxygen in keratinocytes

Categories:

Transfection

Authors:

He YY, Council SE, Feng L, Bonini MG, Chignell CF

In:

Photchem Photobiol (2008) 84(1): 69-74

Nesprin-2 giant safeguards nuclear envelope architecture in LMNA S143F progeria cells

Categories:

Transfection

Authors:

Kandert S, Lüke Y, Kleinhenz T, Neumann S, Lu W, Jaeger VM, Munck M, Wehnert M, Müller CR, Zhou Z, Noegel AA, Dabauvalle MC, Karakesisoglou I

In:

Hum Mol Genet (2007) 16(23): 2944-59

Human papillomavirus type 31 uses a caveolin 1- and dynamin 2-mediated entry pathway for infection of human keratinocytes

Categories:

Transfection

Authors:

Smith JL, Campos SK, Ozbun MA

In:

J Virol (2007) 81(18): 9922-31

Small C-terminal Domain Phosphatases Dephosphorylate the Regulatory Linker Regions of Smad2 and Smad3 to Enhance Transforming Growth Factor-beta Signaling

Categories:

Transfection

Authors:

Wrighton KH, Willis D, Long J, Liu F, Lin X, Feng XH

In:

J Biol Chem (2006) 281(50): 38365-75

Pore-forming Staphylococcus aureusalpha-toxin triggers epidermal growth factor receptor-dependent proliferation

Categories:

Transfection

Authors:

Haugwitz U, Bobkiewicz W, Han SR, Beckmann E, Veerachato G, Shaid S, Biehl S, Dersch K, Bhakdi S, Husmann M

In:

Cell Microbiol (2006) 8(10): 1591-600

ELAC2, a putative prostate cancer susceptibility gene product, potentiates TGF-beta/Smad-induced growth arrest of prostate cells

Categories:

Transfection

Authors:

Noda D, Itoh S, Watanabe Y, Inamitsu M, Dennler S, Itoh F, Koike S, Danielpour D, Ten Dijke P, Kato M

In:

Oncogene (2006) 25(41): 5591-600

The inner nuclear membrane protein Sun1 mediates the anchorage of Nesprin-2 to the nuclear envelope

Categories:

Transfection

Authors:

Padmakumar VC, Libotte T, Lu W, Zaim H, Abraham S, Noegel AA, Gotzmann J, Foisner R and Karakesisoglou I

In:

J Cell Sci (2005) 118(Pt 15): 3419-3430

HAX-1, identified by differential display reverse transcription polyerase chain reaction, is overexpressed in lesional psoriasis

Categories:

Transfection

Authors:

Mirmohammadsadegh A, Tartler U, Michel G, Baer A, Walz M, Wolf R, Ruzicka T and Hengge UR

In:

J Invest Dermatol (2003) 120: 1045-1051

Proteasome inhibition results in TRAIL sensitization of primary keratinocytes by removing the restistance-mediating block of effector caspase maturation

Categories:

Transfection

Authors:

Leverkus M, Sprick MR, Wachter T, Mengling T, Baumann B, Serfling E, Brocker EB, Goebeler M, Neumann M and Walczak H.

In:

Mol Cell Biol (2003) 23(3): 777-790

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