Aortic Smooth Muscle Cells (R-ASM), Rat

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Primary rat smooth muscle cells.

Cell Type:
Smooth muscle cell
Tissue Origin:
aortic
Species:
rat
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Research Area:
Cardiovascular
Cell Characteristics:
Suspension

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
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The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
Smooth muscle, basic D-033 9e5 75% 60% Plasmid (general) 2 µg 100 µl I/II/2b
P1 FP-113 1e5 30-40% good Plasmid (general) 400 ng 20 µl 4D X-Unit

Citations (35)

Differential Regulation of Telomerase Reverse Transcriptase Promoter Activation and Protein Degradation by Histone Deacetylase Inhibition
Categories:
Primary Cells and Media 
Authors:
Qing H, Aono J, Findeisen HM, Jones KL, Heywood EB, Bruemmer D 
In:
J Cell Physiol (2016) 231(6): 1276-82 
Plumericin inhibits proliferation of vascular smooth muscle cells by blocking STAT3 signaling via S-glutathionylation
Categories:
Primary Cells and Media 
Authors:
Heiss EH, Liu R, Waltenberger B, Khan S, Schachner D, Kollmann P, Zimmermann K, Cabaravdic M, Uhrin P, Stuppner H, Breuss JM, Atanasov AG, Dirsch VM 
In:
Scientific Reports (2016) 9(6): ePub 
Nonprenylated Xanthones from Gentiana lutea, Frasera caroliniensis, and Centaurium erythraea as Novel Inhibitors of Vascular Smooth Muscle Cell Proliferation.
Categories:
Primary Cells and Media 
Authors:
Waltenberger B, Liu R, Atanasov AG, Schwaiger S, Heiss EH, Dirsch VM, Stuppner H 
In:
Molecules (2015) 20(11): 20381-20390 
Nifedipine Attenuation of Abdominal Aortic Aneurysm in Hypertensive and non-Hypertensive Mice: Mechanisms and Implications.
Categories:
Primary Cells and Media 
Authors:
Miao XN, Siu KL, Cai H 
In:
J Mol Cell Cardiol (2015) ePub: ePub 
Piperine Congeners as Inhibitors of Vascular Smooth Muscle Cell Proliferation.
Categories:
Primary Cells and Media 
Authors:
Mair CE, Liu R, Atanasov AG, Wimmer L, Nemetz-Fiedler D, Sider N, Heiss EH1, Mihovilovic MD, Dirsch VM1, Rollinger JM 
In:
Other (2015) ePub: ePub 
Loss of fibulin-4 disrupts collagen synthesis and maturation: Implications for pathology resulting from EFEMP2 mutations.
Categories:
Primary Cells and Media 
Authors:
Papke CL, Tsunezumi J, Ringuette LJ, Nagaoka H, Terajima M, Yamashiro Y, Urquhart G, Yamauchi M, Davis EC, Yanagisawa H 
In:
Hum Mol Genet (2015) 28: ePub 
Effects of Parathyroid Hormone-Related Peptide on the Large Conductance Calcium-Activated Potassium Channel and Calcium Homeostasis in Vascular Smooth Muscle Cells
Categories:
Primary Cells and Media 
Authors:
Kamil Mehmet Burgazli, Nikolaus Foerster, Meric Mericliler, Ritvan Chasan, Mariana Parahuleva, Ali Erdogan 
In:
Postgrad Med (2015) 126(2): 76-85 
Capsaicin from chili ( Capsicum spp.) inhibits vascular smooth muscle cell proliferation
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Liu R, Heiss EH, Guo D, Dirsch VM, Atanasov AG2. 
In:
F1000Res (2015) 4: 26 
Identification and characterization of [6]-shogaol from ginger as inhibitor of vascular smooth muscle cell proliferation
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Liu R, Heiss EH, Sider N, Schinkovitz A, Gröblacher B, Guo D, Bucar F, Bauer R, Dirsch VM, Atanasov AG. 
In:
Mol Nutr Food Res (2015) 10: 1002 
Enhanced angiogenesis and increased cardiac perfusion after myocardial infarction in protein tyrosine phosphatase 1B-deficient mice.
Categories:
Primary Cells and Media 
Authors:
Besnier M, Galaup A, Nicol L, Henry JP, Coquerel D, Gueret A, Mulder P, Brakenhielm E, Thuillez C, Germain S, Richard V, Ouvrard-Pascaud A. 
In:
FASEB J (2014) 28(8): 3351-61 
Thrombospondin-1 activation of signal-regulatory protein-a stimulates reactive oxygen species production and promotes renal ischemia reperfusion injury.
Categories:
Primary Cells and Media 
Authors:
Yao M, Rogers NM, Csányi G, Rodriguez AI, Ross MA, St Croix C, Knupp H, Novelli EM, Thomson AW, Pagano PJ, Isenberg JS 
In:
J Am Soc Nephrol (2014) 25(6): 1171-1186 
Sonic hedgehog mediates a novel pathway of PDGF-BB-dependent vessel maturation.
Categories:
Primary Cells and Media 
Authors:
Yao Q, Renault MA, Chapouly C, Vandierdonck S, Belloc I, Jaspard-Vinassa B, Daniel-Lamazière JM, Laffargue M, Merched A, Desgranges C, Gadeau AP. 
In:
Blood (2014) 123(15): ePub 
Identification of Chromomoric Acid C-I as an Nrf2 Activator in Chromolaena odorata.
Categories:
Primary Cells and Media 
Authors:
Heiss EH1, Tran TV, Zimmermann K, Schwaiger S, Vouk C, Mayerhofer B, Malainer C, Atanasov AG, Stuppner H, Dirsch VM. 
In:
Other (2014) 77 (3): 503-8 
Tissue Engineering: Construction of a Multicellular 3D Scaffold for the Delivery of Layered Cell Sheets
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Turner WS, Sandhu N, McCloskey KE 
In:
Journal of Visualized Experiments, JoVE (2014) 92: ePub 
Novel molecular targeting of Necl 5 to prevent intimal hyperplasia and restenosis
Categories:
Primary Cells and Media 
Authors:
Naeem M, Abid R, Murphy T, Park WK, Mills DR 
In:
Other (2014) ePub: ePub 
Comprehensive measurement of respiratory activity in permeabilized cells using extracellular flux analysis.
Categories:
Primary Cells and Media 
Authors:
Salabei JK, Gibb AA, Hill BG 
In:
Nat Protocols (2014) 9(2): 421-438 
Proteomic analysis identifies an NADPH oxidase 1 (Nox1)-mediated role for actin-related protein 2/3 complex subunit 2 (ARPC2) in promoting smooth muscle cell migration.
Categories:
Primary Cells and Media 
Authors:
Al Ghouleh I, Rodríguez A, Pagano PJ, Csányi G. 
In:
Other (2013) 14(10): 20220-20235 
Endogenous CGRP protects against neointimal hyperplasia following wire-induced vascular injury.
Categories:
Primary Cells and Media 
Authors:
Yang L, Sakurai T, Kamiyoshi A, Ichikawa-Shindo Y, Kawate H, Yoshizawa T, Koyama T, Iesato Y, Uetake R, Yamauchi A, Tanaka M, Toriyama Y, Igarashi K, Shindo T. 
In:
Other (2013) 59(2013): 55-66 
Thrombospondin-1 regulates blood flow via CD47 receptor-mediated activation of NADPH oxidase 1.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Csányi G, Yao M, Rodríguez AI, Al Ghouleh I, Sharifi-Sanjani M, Frazziano G, Huang X, Kelley EE, Isenberg JS, Pagano PJ. 
In:
Arterioscler Thromb Vasc Biol (2012) 32(12): 2966-73 
Critical role of the a1-Na+, K +-ATPase subunit in insensitivity of rodent cells to cytotoxic action of ouabain.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Akimova OA, Tverskoi AM, Smolyaninova LV, Mongin AA, Lopina OD, La J, Dulin NO, Orlov SN 
In:
Apoptosis (2012) ePub: e 
Pharmacologic effects of 2-methoxyestradiol on angiotensin type 1 receptor down-regulation in rat liver epithelial and aortic smooth muscle cells
Categories:
Primary Cells and Media 
Authors:
Koganti S, Snyder R, Thekkumkara T 
In:
Gender Medicine (2012) 9(2): 76-93 
RNA interference-produced autoregulation of inducible nitric oxide synthase expression
Categories:
Primary Cells and Media 
Authors:
Feng C, Cao L, Zuo Z. 
In:
FEBS Lett (2011) 585(15): 2488-92 
Glucocorticoid-related signaling effects in vascular smooth muscle cells
Categories:
Transfection 
Authors:
Molnar GA, Lindschau C, Dubrovska G, Mertens PR, Kirsch T, Quinkler M, Gollasch M, Wresche S, Luft FC, Muller DN, Fiebeler A 
In:
Hypertension (2008) 51(5): 1372-8 
The benefit of docosahexanoic acid on the migration of vascular smooth muscle cells is partially dependent on Notch regulation of MMP-2/-9
Categories:
Transfection 
Authors:
Delbosc S, Glorian M, Le Port AS, Bereziat G, Andreani M, Limon I 
In:
Am J Pathol (2008) 172(5): 1430-40 
Inhibition of interleukin-1beta-induced group IIA secretory phospholipase A2 expression by peroxisome proliferator-activated receptors (PPARs) in rat vascular smooth muscle cells: cooperation between PPARbeta and the proto-oncogene BCL-6
Categories:
Transfection 
Authors:
Ravaux L, Denoyelle C, Monne C, Limon I, Raymondjean M, Hadri KE 
In:
Mol Cell Biol (2007) 27(23): 8374-87 
Apolipoprotein E Interrupts Interleukin-1 Signaling in Vascular Smooth Muscle Cells
Categories:
Transfection 
Authors:
Kawamura A, Baitsch D, Telgmann R, Feuerborn R, Weissen-Plenz G, Hagedorn C, Saku K, Brand-Herrmann SM, von Eckardstein A, Assmann G, Nofer JR 
In:
Arterioscler Thromb Vasc Biol (2007) 27(7): 1610-7 
Cooperation of SRC-1 and p300 with NF-kappaB and CREB in angiotensin II-induced IL-6 expression in vascular smooth muscle cells
Categories:
Transfection 
Authors:
Sahar S, Reddy MA, Wong C, Meng L, Wang M, Natarajan R 
In:
Arterioscler Thromb Vasc Biol (2007) 27(7): 1528-34 
Adrenaline increases glucose transport via a Rap1-p38MAPK pathway in rat vascular smooth muscle cells
Categories:
Transfection 
Authors:
Kanda Y, Watanabe Y 
In:
Br J Pharmacol (2007) 151(4): 476-82 
C-Peptide Induces Smooth Muscle cell Proliferation - Involvement of Src-Kinase, Phosphatidylinositol 3-Kinase and Extracellular Signal-Regulated Kinase 1/2
Categories:
Transfection 
Authors:
Walcher D, Babiak C, Poletek P, Rosenkranz S, Bach H, Betz S, Durst R, Grub M, Hombach V, Strong J, Marx N 
In:
Circ Res (2006) 99(11): 1181-7 
Angiotensin II Stimulates Transcription of Insulin-like Growth Factor 1 Receptor in Vascular Smooth Muscle Cells: Role of NF-B
Categories:
Transfection 
Authors:
Ma Y, Zhang L, Peng T, Cheng J, Taneja S, Zhang J, Delafontaine P and Du J 
In:
Endocrinology (2006) 147(3): 1256-63 
17Beta-estradiol attenuates PDGF signaling in vascular smooth muscle cells at the postreceptor level
Categories:
Transfection 
Authors:
Kappert K, Caglayan E, Huntgeburth M, Baumer AT, Sparwel J, Uebel M and Rosenkranz S 
In:
Am J Physiol Heart Circ Physiol (2006) 290(2): H538-46 
Dual pathways for nuclear factor kappaB activation by angiotensin II in vascular smooth muscle: phosphorylation of p65 by IkappaB kinase and ribosomal kinase
Categories:
Transfection 
Authors:
Zhang L, Cheng J, Ma Y, Thomas W, Zhang J, Du J 
In:
Circ Res (2005) 97(10): 975-82 
Aldosterone stimulates vascular smooth muscle cell proliferation via big mitogen-activated protein kinase 1 activation
Categories:
Transfection 
Authors:
Ishizawa K, Izawa Y, Ito H, Miki C, Miyata K, Fujita Y, Kanematsu Y, Tsuchiya K, Tamaki T, Nishiyama A and Yoshizumi M 
In:
Hypertension (2005) 46(4): 1046-1052 
Src and Cas are essentially but differentially involved in angiotensin II-stimulated migration of vascular smooth muscle cells via extracellular signal-regulated kinase 1/2 and c-Jun NH2-terminal kinase activation
Categories:
Transfection 
Authors:
Kyaw M, Yoshizumi M, Tsuchiya K, Kagami S, Izawa Y, Fujita Y, Ali N, Kanematsu Y, Toida K, Ishimura K and Tamaki T 
In:
Mol Pharmacol (2004) 65(4): 832-841 
Coordinate notch 3-hairy related transscription factor pathway regulation in response to arterial injury: mediator role of PDGF and ERK
Categories:
Transfection 
Authors:
Wang W, Campos AH, Prince CZ, Mou Y and Pollmann MJ 
In:
J Biol Chem (2002) 277(26): 23165-23171 
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