OCI-AML3

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acute myelogenous leukemia

Cell Type:
Myeloid (unspec.)
Tissue Origin:
blood
Species:
human
Research Area:
Cancer Research/Cell Biology
Immunotherapy / Hematology
Cell Characteristics:
Suspension

Recommended Media

MEM Alpha Eagle

MEM Alpha was developed in 1971 and differs from standard MEM by containing the following unique components:  vitamin B12, ascorbic acid, non-essential amino acids, sodium pyruvate, lipoic acid, and D-biotin.
This media has been used to culture bone marrow cells and amniotic cells for chromosome analysis.

MEM Alpha is available as:
12-169 - without L-glutamine, deoxyribonucleosides or ribonucleosides
BE02-002 - with Ultraglutamine, deoxyribonucleosides and ribonucleosides

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
T X-001 3e6 60% 90% Plasmid (general) 2 µg 100 µl I/II/2b
T T-020 3e6 50% 50% Plasmid (general) 2 µg 100 µl I/II/2b

Citations

Generation of locus-specific degradable tag knock-ins in mouse and human cell lines
Categories:
Transfection 
Authors:
Damhofer H, Radzisheuskaya A, Helin K. 
In:
STAR Protoc (2021) 2(2): 100575 
TALENs-mediated gene disruption of FLT3 in leukemia cells: Using genome-editing approach for exploring the molecular basis of gene abnormality.
Categories:
Transfection 
Authors:
Wang J, Li T, Zhou M, Hu Z, Zhou X, Zhou S, Wang N, Huang L, Zhao L, Cao Y, Xiao M, Ma D, Zhou P, Shang Z, Zhou J 
In:
Scientific Reports (2015) 5:18454: 10.1038 
Convergence of hypoxia and TGFß pathways on cell cycle regulation in human hematopoietic stem/progenitor cells
Categories:
Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Wierenga AT, Vellenga E, Schuringa JJ 
In:
PLoS ONE (2014) 9(3): e93494 
Suppressor of Cytokine Signaling 6 (SOCS6) Negatively Regulates Flt3 Signal Transduction through Direct Binding to Phosphorylated Tyrosines 591 and 919 of Flt3
Categories:
Transfection 
Authors:
Kazi J, Sun J, Phung B, Zadjali F, Flores-Morales A, Ronnstrand L 
In:
J Biol Chem (2012) 287: 36509-36517 
The warburg effect in leukemia-stroma cocultures is mediated by mitochondrial uncoupling associated with uncoupling protein 2 activation
Categories:
Transfection 
Authors:
Samudio I, Fiegl M, McQueen T, Clise-Dwyer K, Andreeff M 
In:
Cancer Res (2008) 68(13): 5198-205 
Triptolide induces caspase-dependent cell death mediated via the mitochondrial pathway in leukemic cells
Categories:
Transfection 
Authors:
Carter BZ, Mak DH, Schober WD, McQueen T, Harris D, Estrov Z, Evans RL, Andreeff M 
In:
Blood (2006) 108(2): 630-7 

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