Macrophage, mouse

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Bone-marrow derived primary immune cells.

Cell Type:
Macrophage
Tissue Origin:
bone marrow
Species:
mouse
Research Area:
Cancer Research/Cell Biology
Immunotherapy / Hematology
Cell Characteristics:
Suspension

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
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The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
Macrophage, mouse Y-001 1e6 60% good Plasmid (general) 2 µg 100 µl I/II/2b

Citations (18)

Biocompatibility Assessment of a New Biodegradable Vascular Graft via In Vitro Co-culture Approaches and In Vivo Model.
Categories:
Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Enayati M, Eilenberg M, Grasl C, Riedl P, Kaun C, Messner B, Walter I, Liska R, Schima H, Wojta J, Podesser BK, Bergmeister H 
In:
Anal Biochem (2016) 44(11): 3319-3334 
Macrophage PI3K? Drives Pancreatic Ductal Adenocarcinoma Progression
Categories:
Primary Cells and Media 
Authors:
Kaneda MM, Cappello P, Nguyen AV, Ralainirina N, Hardamon CR, Foubert P, Schmid MC, Sun P, Mose E, Bouvet M, Lowy AM, Valasek MA, Sasik R, Novelli F, Hirsch E, Varner JA 
In:
Cancers (2016) 6(8): 870-885 
FOXO1 mediates RANKL-induced osteoclast formation and activity.
Categories:
Primary Cells and Media 
Authors:
Wang Y, Dong G, Jeon HH, Elazizi M, La LB, Hameedaldeen A, Xiao E, Tian C, Alsadun S, Choi Y, Graves DT. 
In:
J Immunol (2015) 194(6): 2878-87 
Human macrophage SCN5A activates an innate immune signaling pathway for antiviral host defense.
Categories:
Primary Cells and Media 
Authors:
Jones A, Kainz D, Khan F, Lee C, Carrithers MD 
In:
J Biol Chem (2014) 289(51): 35326-40 
Comparative in vitro study of four commercial biomaterials used for bone grafting.
Categories:
Primary Cells and Media, Bioassay  
Authors:
Bosetti M, Bianchi AE, Zaffe D, Cannas M 
In:
Other (2013) 11(2): 80-8 
Three-dimensional FRET reconstruction microscopy for analysis of dynamic molecular interactions in live cells
Categories:
Transfection 
Authors:
Hoppe AD, Shorte SL, Swanson JA, Heintzmann R 
In:
Biophys J (2008) 95(1): 400-18 
SHIP-1 increases early oxidative burst and regulates phagosome maturation in macrophages
Categories:
Transfection 
Authors:
Kamen LA, Levinsohn J, Cadwallader A, Tridandapani S, Swanson JA 
In:
J Immunol (2008) 180(11): 7497-505 
NMD is essential for hematopoietic stem and progenitor cells and for eliminating by-products of programmed DNA rearrangements
Categories:
Transfection 
Authors:
Weischenfeldt J, Damgaard I, Bryder D, Theilgaard-Mönch K, Thoren LA, Nielsen FC, Jacobsen SE, Nerlov C, Porse BT 
In:
Genes Dev (2008) 22(10): 1381-96 
TRAM couples endocytosis of Toll-like receptor 4 to the induction of interferon-beta
Categories:
Transfection 
Authors:
Kagan JC, Su T, Horng T, Chow A, Akira S, Medzhitov R 
In:
Nat Immunol (2008) 9(4): 361-8 
Phospholipase C beta3 deficiency leads to macrophage hypersensitivity to apoptotic induction and reduction of atherosclerosis in mice
Categories:
Transfection 
Authors:
Wang Z, Liu B, Wang P, Dong X, Fernandez-Hernando C, Li Z, Hla T, Li Z, Claffey K, Smith JD, Wu D 
In:
J Clin Invest (2008) 118(1): 195-204 
Irf3 polymorphism alters induction of interferon beta in response to Listeria monocytogenes infection
Categories:
Transfection 
Authors:
Garifulin O, Qi Z, Shen H, Patnala S, Green MR, Boyartchuk V 
In:
PLoS Genet (2007) 3(9): 1587-97 
Ferroxidase activity is required for the stability of cell surface ferroportin in cells expressing GPI-ceruloplasmin
Categories:
Transfection 
Authors:
De Domenico I, Ward DM, di Patti MC, Jeong SY, David S, Musci G, Kaplan J 
In:
EMBO J (2007) 26(12): 2823-31 
TLR-Dependent Induction of IFN-beta Mediates Host Defense against Trypanosoma cruzi
Categories:
Transfection 
Authors:
Koga R, Hamano S, Kuwata H, Atarashi K, Ogawa M, Hisaeda H, Yamamoto M, Akira S, Himeno K, Matsumoto M, Takeda K 
In:
J Immunol (2006) 177(10): 7059-66 
Reduction in ABCG1 in type 2 diabetic mice increases macrophage foam cell formation
Categories:
Transfection 
Authors:
Mauldin JP, Srinivasan S, Mulya A, Gebre A, Parks JS, Daugherty A, Hedrick CC 
In:
J Biol Chem (2006) 281(30): 21216-24 
Phosphoinositide-mediated adaptor recruitment controls Toll-like receptor signaling
Categories:
Transfection 
Authors:
Kagan JC, Medzhitov R 
In:
Cell (2006) 125(5): 943-55 
The inositol phosphatase SHIP-2 down-regulates FcgammaR-mediated phagocytosis in murine macrophages independently of SHIP-1
Categories:
Transfection 
Authors:
Ai J, Maturu A, Johnson W, Wang Y, Marsh CB and Tridandapani S 
In:
Blood (2006) 107(2): 813-820 
Legionella subvert the functions of rab1 and sec22b to create a replicative organelle
Categories:
Transfection 
Authors:
Kagan JC, Stein MP, Pypaert M and Roy CR 
In:
J Exp Med (2004) 199(9): 1201-1211 
B-Myb-Dependent Regulation of c-Myc Expression by Cytosolic Phospholipase A2
Categories:
Transfection 
Authors:
Tashiro S, Sumi T, Uozumi N, Shimizu T and Nakamura T 
In:
J Biol Chem (2004) 279(17): 17715-17722 
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