MC3T3-E1
Export
embryonal pre-osteoblasts derived from C57BL/6 mice
Cell Type:
Bone
Tissue Origin:
bone
Species:
mouse
Cell Characteristics:
Adherent
Transfection Information
Lonza Optimized Protocol
Optimization Guideline
Filter:
Any Substrate
Any Platform
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols
or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our
optimization strategy
to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol
Kit
Program
Cells
Efficiency
Viable Cells
Substrate
Format
Platform
T
T-016
1e6
20%
95%
Plasmid (general)
2 µg
100 µl
I/II/2b
T
T-020
1e6
20%
95%
Plasmid (general)
2 µg
100 µl
I/II/2b
T
T-027
1e6
20%
95%
Plasmid (general)
2 µg
100 µl
I/II/2b
Citations
Different skeletal effects of the peroxisome proliferator activated receptor (PPAR)alpha agonist fenofibrate and the PPARgamma agonist pioglitazone.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media, Bioassay
Authors:
Syversen U, Stunes AK, Gustafsson BI, Obrant KJ, Nordsletten L, Berge R, Thommesen L, Reseland JE
In:
Other (2009) 9:10: 1-13
Polycystin-1 regulates skeletogenesis through stimulation of the osteoblast-specific transcription factor Runx2-II
Categories:
Transfection
Authors:
Xiao Z, Zhang S, Magenheimer BS, Luo J, Quarles LD
In:
J Biol Chem (2008) 283(18): 12624-34
Direct interactions of Runx2 and canonical Wnt signaling induce FGF18
Categories:
Transfection
Authors:
Reinhold MI, Naski MC
In:
J Biol Chem (2007) 282(6): 3653-63
Cilia-like Structures and Polycystin-1 in Osteoblasts/Osteocytes and Associated Abnormalities in Skeletogenesis and Runx2 Expression
Categories:
Transfection
Authors:
Xiao Z, Zhang S, Mahlios J, Zhou G, Magenheimer BS, Guo D, Dallas SL, Maser R, Calvet JP, Bonewald L, Quarles LD
In:
J Biol Chem (2006) 281(41): 30884-30895
Carbon monoxide and nitric oxide protect against tumor necrosis factor-alpha-induced apoptosis in osteoblasts: HO-1 is necessary to mediate the protection
Categories:
Transfection
Authors:
Chae HJ, Chin HY, Lee GY, Park HR, Yang SK, Chung HT, Pae HO, Kim HM, Chae SW, Kim HR
In:
Clin Chim Acta (2006) 365(1-2): 270-8