CHO-DG44 (DHFR-)

chinese hamster ovary; DHFR- clone

Cell Type:
Ovary Epith.
Tissue Origin:
ovarian
Species:
hamster
Cell Characteristics:
Suspension

Recommended Media

Developed for low density (clonal) growth of CHO cells.

Storage = 2ºC to 8ºC

12-618 = with L-glutamine
BE02-014 = with UltraGlutamine I, without thymidine

Ham's F12 Medium is a nutrient mixture designed to cultivate a wide variety of mammalian and hybridoma cells (including low density (clonal) growth of CHO cells) when used with serum in combination with hormones and transferrin.

ProCHO™ Protein-free CHO Media were developed specifically to facilitate the production and downstream processing of recombinant proteins expressed in CHO cells. These protein-free formulations support high-density cultures without the need for animal derived components.
Very low levels of recombinant insulin facilitate both downstream purification and regulatory compliance.

The following media systems are available:
- ProCHO™ 4 Medium – For concurrent transition of adherent CHO cells to serum-free and suspension culture; supports faster doubling times
- ProCHO™ 5 Medium – For CHO cells already growing in suspension; supports increased protein production
- ProCHO™ AT Medium – For adherent culture of CHO cells

Storage = 2°C to 8°C

12-029Q - ProCHO™ 4 with phenol red and 0.1% Pluronic® F-68; but without L-glutamine, hypoxanthine, or thymidine.
04-919Q - ProCHO™ 4 with 0.1% Pluronic® F-68; and without phenol red, L-glutamine, hypoxanthine, or thymidine.

12-766Q - ProCHO™ 5  with 0.1% Pluronic® F-68; and without L-glutamine, phenol red, hypoxanthine, or thymidine.
BE15-766 - ProCHO™ 5 powder without phenol red

BE02-016 - ProCHO™-AT contains L-glutamine and does not contain hypoxanthine or thymidine.

PowerCHO™ Chemically Defined, Serum-free and hydrolysate-free CHO Media are non-animal origin media designed to support the growth of CHO cell lines, particularly high-density suspension cultures.

For therapeutic bioprocessing applications, these protein-free formulations also facilitate both downstream purification and regulatory compliance.

Storage = 2°C to 8°C

12-770Q - PowerCHO™-1 (with HEPES and Pluronic® F-68, without phenol red, hypoxanthine, thymidine, or L-glutamine)
12-771Q - PowerCHO™-2 (with HEPES and Pluronic® F-68, without phenol red, hypoxanthine, thymidine, or L-glutamine)
BE15-771N - PowerCHO™-2 powder (with HEPES and Pluronic® F-68, without phenol red, hypoxanthine, thymidine, or L-glutamine)
12-772Q - PowerCHO™-3 (with HEPES and Pluronic® F-68, without phenol red, hypoxanthine, thymidine, or L-glutamine)

BE12-776Q - PowerCHO™-GS (with HEPES and Pluronic® F-68, without insulin, phenol red or L-glutamine)
PowerCHO™ -GS is designed for use with Lonza's Proprietary GS Gene Expression System™.

12-929Q - PowerCHO™ - Advance

ProFreeze™-CDM, NAO, Chemically Defined Freeze Medium (2X) is universally suitable for cryopreserving many cell types in the absence of fetal bovine serum (FBS), which is particularly advantageous for freezing of cells cultured in a serum-free and animal component-free environment.
This protein-free freezing medium contains no animal derived components, insulin, or hydrolysate, and maintains high cell viability upon recovery from frozen storage.

ProFreeze™ Medium requires the addition of 15% reagent or spectrophotometric grade dimethylsulfoxide (DMSO) at time of use.

Storage = 2°C to 8°C

MEM Alpha was developed in 1971 and differs from standard MEM by containing the following unique components:  vitamin B12, ascorbic acid, non-essential amino acids, sodium pyruvate, lipoic acid, and D-biotin.
This media has been used to culture bone marrow cells and amniotic cells for chromosome analysis.

MEM Alpha is available as:
12-169 - without L-glutamine, deoxyribonucleosides or ribonucleosides
BE02-002 - with Ultraglutamine, deoxyribonucleosides and ribonucleosides

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
V U-024 1e6 90% 95% Plasmid (general) 2 µg 100 µl I/II/2b
V U-030 1e6 93% 95% Plasmid (general) 2 µg 100 µl I/II/2b
SG FF-137 4e5 95% 80% Plasmid (general) 400 ng 20 µl Shuttle
SG DT-137 4e5 90% 95% Plasmid (general) 400 ng 20 µl Shuttle

Citations

Categories:
Classical Media and Reagents, Serum-free and Speciality Media, Transfection 
Authors:
Muller N1, Derouazi M, Van Tilborgh F, Wulhfard S, Hacker DL, Jordan M, Wurm FM. 
In:
Biotechnol Lett (2007) 29 (5): 703-11 
Categories:
Transfection 
Authors:
Rosenblum MD, Woodliff JE, Madsen NA, McOlash LJ, Keller MR and Truitt RL 
In:
J Invest Dermatol (2005) 125(6): 1130-1138