Adipocyte (pre), human

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Preadipocytes are precursor cells that develop into adipocytes when fully differentiated. Adipocytes perform essential functions of energy metabolism and are characterized by the accumulation of intracellular triglycerides.

Preadipocyte Cells are isolated from subcutaneous or visceral fat. Subcutaneous fat is often found attached to skin in the lower abdomen area. Visceral preadipocytes are isolated from adipose tissue associated with internal organs, such as the bladder or kidney.

Relative to subcutaneous fat, visceral fat deposits are mobilized at a higher rate to produce serum fatty acids which contribute to insulin resistance, Diabetes Type 2, and other related cardiovascular disorders.

Cell Type:
Adipocyte
Tissue Origin:
adipose
Species:
human
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Cell Characteristics:
Adherent

Recommended Media

Preadipocyte Growth Medium-2

Our Preadipocyte Growth Media-2 (PGM™-2) is optimized for preadipocyte proliferation, and differentiation in only 10 days of culture time. The media contains no glitazones that can interfere with PPAR-gamma activity measurements. The media used with our cells compose a robust system that requires no media changes during the culture time to differentiate.

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
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The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
Fibroblast, basic V-033 8e5 40% 50-60% Plasmid (general) 3 µg 100 µl I/II/2b
Fibroblast, basic U-033 8e5 30% 70-80% Plasmid (general) 3 µg 100 µl I/II/2b
V A-020 3e5 60-70% 70% Plasmid (general) µg 100 µl I/II/2b
P1 CA-137 5e5 59-82% 58-78% Plasmid (general) 2 µg 100 µl 4D X-Unit
P1 CA-158 5e5 55-91% 38-89% Plasmid (general) 2 µg 100 µl 4D X-Unit
P1 CA-137 1e5 37-85% 34-84% Plasmid (general) 400 ng 20 µl 4D X-Unit
P1 CA-158 1e5 56-94% 42-92% Plasmid (general) 400 ng 20 µl 4D X-Unit

Citations (34)

Novel anti-obesity effects of beer hops compound xanthohumol: role of AMPK signaling pathway.
Categories:
Primary Cells and Media 
Authors:
Samuels JS1, Shashidharamurthy R1, Rayalam S1 
In:
Other (2018) 15: 42 
Reciprocal Inflammatory Signaling Between Intestinal Epithelial Cells and Adipocytes in the Absence of Immune Cells.
Categories:
Primary Cells and Media 
Authors:
Takahashi Y, Sato S, Kurashima Y, Lai CY, Otsu M, Hayashi M, Yamaguchi T, Kiyono H 
In:
EBioMedicine (2017) 23: 34-45 
Reciprocal Inflammatory Signaling Between Intestinal Epithelial Cells and Adipocytes in the Absence of Immune Cells.
Categories:
Primary Cells and Media 
Authors:
Takahashi Y1, Sato S2, Kurashima Y3, Lai CY4, Otsu M4, Hayashi M5, Yamaguchi T5, Kiyono H6. 
In:
EBioMedicine (2017) 23: 34-45 
Thyroid hormone receptor sumoylation is required for preadipocyte differentiation and proliferation.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media, Transfection 
Authors:
Liu YY, Ayers S, Milanesi A, Teng X, Rabi S, Akiba Y, Brent GA 
In:
J Biol Chem (2015) 290(12): 7402-15 
Chronic superantigen exposure induces systemic inflammation, elevated bloodstream endotoxin, and abnormal glucose tolerance in rabbits: possible role in diabetes.
Categories:
Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Vu BG, Stach CS, Kulhankova K, Salgado-Pabón W, Klingelhutz AJ, Schlievert PM 
In:
mBio (2015) 6(2): ePub 
Interleukin-10 receptor-1 expression in monocyte-derived antigen-presenting cell populations: dendritic cells partially escape from IL-10\'s inhibitory mechanisms.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
von Lanzenauer SH, Wolk K, Höflich C, Kunz S, Grünberg BH, Döcke WD, Reineke U, Asadullah K, Sterry W, Volk HD, Sabat R 
In:
Genes Immun (2015) 16(1): ePub 
Anti-Adipogenic Polyphenols of Water Shield Suppress TNF-a-Induced Cell Damage and Enhance Expression of HAS2 and HAPB2 in Adiponectin- Treated Dermal Fibroblasts
Categories:
Primary Cells and Media 
Authors:
Shimoda1 H, Nakamura S, Hitoe S, Terazawa S, Tanaka J, Matsumoto T, Matsuda H 
In:
Other (2014) 2(5): 1-7 
Apolipoprotein A-I truncations in Chagas disease are caused by cruzipain, the major cysteine protease of Trypanosoma cruzi
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Miao Q, Santamaria C, Bailey D, Genest J, Ward BJ, Ndao M 
In:
Am J Pathol (2014) 184(4): 976-84 
Statins impair glucose uptake in human cells.
Categories:
Primary Cells and Media 
Authors:
Nowis D, Malenda A, Furs K, Oleszczak B, Sadowski R, Chlebowska J, Firczuk M, Bujnicki JM, Staruch AD, Zagozdzon R, Glodkowska-Mrowka E, Szablewski L, Golab J 
In:
Other (2014) 2(1): ePub 
On the formation of lipid droplets in human adipocytes: the organization of the perilipin-vimentin cortex.
Categories:
Primary Cells and Media 
Authors:
Heid H, Rickelt S, Zimbelmann R, Winter S, Schumacher H, Dörflinger Y, Kuhn C, Franke WW. 
In:
PLoS ONE (2014) 9(2): 1371 
KDT501, a derivative from hops, normalizes glucose metabolism and body weight in rodent models of diabetes
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Konda VR, Desai A, Darland G, Grayson N, Bland JS 
In:
PLoS ONE (2014) 9(1): ePub 
AMPK Activation through Mitochondrial Regulation Results in Increased Substrate Oxidation and Improved Metabolic Parameters in Models of Diabetes
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Jenkins Y, Sun TQ, Markovtsov V, Foretz M, Li W, Nguyen H, Li Yi, Pan A, Uy G, Gross L, Baltgalvis K, Yung SL, Gururaja T, Kinoshita T, Owyang A, Smith IJ, McCaughey K, White K, Godinez G, Alcantara R 
In:
PLoS ONE (2013) 8(12): ePub 
Apelin inhibits diet-induced obesity by enhancing lymphatic and blood vessel integrity
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Sawane M, Kajiya K, Kidoya H, Takagi M, Muramatsu F, Takakura N. 
In:
Diabetes (2013) 62(6): 1970-1980 
Extending human hematopoietic stem cell survival in vitro with adipocytes.
Categories:
 
Authors:
Glettig DL, Kaplan DL. 
In:
BioResearch Open Access (2013) 2(3): 179-85 
Adipocytes as a source of increased circulating levels of nicotinamide phosphoribosyltransferase/visfatin in active acromegaly
Categories:
Primary Cells and Media, Bioassay  
Authors:
Olarescu NC, Ueland T, Lekva T, Dahl TB, Halvorsen B, Aukrust P, Bollerslev J. 
In:
J Clin Endocrinol Metab (2012) 97(4): 1355-1362 
Peptides Derived from Rice Bran Protect Cells from Obesity and Alzheimer's Disease
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Kannan A, Hettiarachchy NS, Mahedevan M 
In:
Other (2012) 3(3): 131-135 
Increased serum and bone matrix levels of transforming growth factor 1 in patients with GH deficiency in response to GH treatment
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media, Bioassay  
Authors:
Ueland T, Lekva T, Otterdal K, Dahl TB, Olarescu NC, Jørgensen AP, Fougner KJ, Brixen K, Aukrust P, Bollerslev J 
In:
Eur J Endocrinol (2011) 165(3): 393-400 
Impact of metabolic regulators on the expression of the obesity associated genes FTO and NAMPT in human preadipocytes and adipocytes.
Categories:
Primary Cells and Media 
Authors:
Friebe D, Löffler D, Schönberg M, Bernhard F, Büttner P, Landgraf K, Kiess W, Körner A. 
In:
PLoS ONE (2011) 6(6): ePub 
Human primary adipocytes exhibit immune cell function: adipocytes prime inflammation independent of macrophages.
Categories:
Primary Cells and Media 
Authors:
Meijer K, de Vries M, Al-Lahham S, Bruinenberg M, Weening D, Dijkstra M, Kloosterhuis N, van der Leij RJ, van der Want H, Kroesen BJ, Vonk R, Rezaee F. 
In:
PLoS ONE (2011) 6(3): ePub 
Human bone marrow adipocytes support dexamethasone-induced osteoclast differentiation and function through RANKL expression
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Goto H, Osaki M, Fukushima T, Sakamoto K, Hozumi A, Baba H, Shindo H. 
In:
Biomed Res Int. (2011) 32(1): 37-44 
Antiadipogenic effects of the mineralocorticoid receptor antagonist drospirenone: potential implications for the treatment of metabolic syndrome.
Categories:
Primary Cells and Media 
Authors:
Caprio M, Antelmi A, Chetrite G, Muscat A, Mammi C, Marzolla V, Fabbri A, Zennaro MC, Fève B 
In:
Endocrinology (2011) 152(1): 113-125 
Supercritical fluid extraction of oregano (Origanum vulgare) essentials oils: anti-inflammatory properties based on cytokine response on THP-1 macrophages
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Ocaña-Fuentes A, Arranz-Gutiérrez E, Señorans FJ, Reglero G. 
In:
Other (2010) 48(6): 1568-1575 
In vitro study of the effect of diesterified alkoxyglycerols with conjugated linoleic acid on adipocyte inflammatory mediators
Categories:
 
Authors:
Ocaña A, Gómez-Asensio C, Arranz-Gutiérrez E, Torres C, Señoráns FJ, Reglero G. 
In:
Lipids in Health and Disease (2010) 9(36): ePub 
Down-regulation of tissue inhibitor of metalloproteinase-3 (TIMP-3) expression is necessary for adipocyte differentiation.
Categories:
Primary Cells and Media, Transfection 
Authors:
Bernot D, Barruet E, Poggi M, Bonardo B, Alessi MC, Peiretti F. 
In:
J Biol Chem (2010) 285(9): 6508-14 
Despite IFN-lambda receptor expression, blood immune cells, but not keratinocytes or melanocytes, have an impaired response to type III interferons: implications for therapeutic applications of these cytokines.
Categories:
Primary Cells and Media 
Authors:
Witte K, Gruetz G, Volk HD, Looman AC, Asadullah K, Sterry W, Sabat R, Wolk K. 
In:
Genes Immun (2009) 10(8): 702-714 
Angiotensin-converting enzyme (ACE) inhibitors modulate cellular retinol-binding protein 1 and adiponectin expression in adipocytes via the ACE-dependent signaling cascade.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Kohlstedt K, Gershome C, Trouvain C, Hofmann WK, Fichtlscherer S, Fleming I. 
In:
Mol Pharmacol (2009) 75(3): 685-92 
Conjugated linoleic acid-mediated inflammation and insulin resistance in human adipocytes are attenuated by resveratrol
Categories:
Transfection 
Authors:
Kennedy A, Overman A, Lapoint K, Hopkins R, West T, Chuang CC, Martinez K, Bell D, McIntosh MK 
In:
J Lipid Res (2008) epub: online 
Trans-10, cis-12 conjugated linoleic acid antagonizes ligand-dependent PPARgamma activity in primary cultures of human adipocytes
Categories:
Transfection 
Authors:
Kennedy A, Chung S, LaPoint K, Fabiyi O, McIntosh MK 
In:
J Nutr (2008) 138(3): 455-61 
The differential interactions of peroxisome proliferator-activated receptor gamma ligands with Tyr473 is a physical basis for their unique biological activities.
Categories:
Bioassay  
Authors:
Einstein M, Akiyama TE, Castriota GA, Wang CF, McKeever B, Mosley RT, Becker JW, Moller DE, Meinke PT, Wood HB, Berger JP. 
In:
Mol Pharmacol (2008) 73(1): 62-74 
Human progenitor cells from bone marrow or adipose tissue produce VEGF, HGF, and IGF-I in response to TNF by a p38 MAPK-dependent mechanism.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Wang M, Crisostomo PR, Herring C, Meldrum KK, Meldrum DR. 
In:
Am J Physiol Regul Integr Comp Physiol (2006) 291(4): R880-4 
Exosome-Derived miR-25-3p and miR-92a-3p Stimulate Liposarcoma Progression.
Categories:
Primary Cells and Media 
Authors:
Casadei L1,2, Calore F3, Creighton CJ4, Guescini M5, Batte K1,2, Iwenofu OH6, Zewdu A1,2, Braggio DA1,2, Bill KL1,2, Fadda P3, Lovat F3, Lopez G1,2, Gasparini P3, Chen JL1,2, Kladney RD3,7,8, Leone G3,7,8, Lev D9, Croce CM10, Pollock RE11,2 
In:
Cancer Res () 77: 3846-3856 
IL-6-mediated cross-talk between human preadipocytes and ductal carcinoma in situ in breast cancer progression.
Categories:
Primary Cells and Media 
Authors:
Kim HS1, Jung M1, Choi SK1,2, Woo J1, Piao YJ1,2, Hwang EH1, Kim H1,2, Kim SJ3, Moon WK4,5. 
In:
J Exp Clin Cancer Res () 37: 200 
Omentin-regulated proteins combine a pro-inflammatory phenotype with an anti-inflammatory counterregulation in human adipocytes: A proteomics analysis.
Categories:
Primary Cells and Media 
Authors:
Niersmann C1,2, Hauck SM2,3, Kannenberg JM1,2, Röhrig K1,2, von Toerne C2,3, Roden M1,2,4, Herder C1,2,5, Carstensen-Kirberg M1,2 
In:
Other () : e3074 
miR-133a function in the pathogenesis of dedifferentiated liposarcoma.
Categories:
Primary Cells and Media 
Authors:
Yu PY1,2, Lopez G1,3, Braggio D1,3, Koller D1,3, Bill KLJ1,3, Prudner BC4, Zewdu A1,3, Chen JL1,5, Iwenofu OH1,6, Lev D7, Strohecker AM1,3,8, Fenger JM9, Pollock RE1,3, Guttridge DC1,8 
In:
Cancer Cell () 18: 89 
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