cells

T-cell lymphoma

Cell Type:

T Cell

Tissue Origin:

blood

Species:

human

Research Area:

Cancer Research/Cell Biology

Immunotherapy / Hematology

Cell Characteristics:

Suspension

Transfection Information

Lonza Optimized Protocol
Optimization Guideline

Filter:

The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.

Kit	Program	Cells	Efficiency	Viable Cells	Substrate		Format	Platform
V	O-017	1e6	68%	88%	Plasmid (general)	2 µg	100 µl	I/II/2b
V	T-016	1e6	64%	74%	Plasmid (general)	2 µg	100 µl	I/II/2b
V	U-020	5e6	31%	95%	Plasmid (general)	3 µg	100 µl	I/II/2b
SF	CM-150	6e5	46%	85%	Plasmid (general)	0.4 µg	20 µl	4D X-Unit
SF	CA-137	6e5	65.8%	69.7%	Plasmid (general)	0.4 µg	20 µl	4D X-Unit

Citations

Cancer translocations in human cells induced by zinc finger and TALE nucleases.

Categories:

Transfection

Authors:

Piganeau M, Ghezraoui H, De Cian A, Guittat L, Tomishima M, Perrouault L, René O, Katibah GE, Zhang L, Holmes MC, Doyon Y, Concordet JP, Giovannangeli C, Jasin M, Brunet E.

In:

Genome Res (2013) 23(7): 1182-93

Cooperative binding of the MHC-I cytoplasmic domain and HIV-1 Nef to the endosomal AP-1 complex via its micro subunit

Categories:

Transfection

Authors:

Noviello CM, Benichou S, Guatelli JC

In:

J Virol (2008) 82(3): 1249-58

Target cell APOBEC3C can induce limited G-to-A mutation in HIV-1

Categories:

Transfection

Authors:

Bourara K, Liegler TJ, Grant RM

In:

PLoS Pathog (2007) 3(10): 1477-85

Maintenance of Nef-mediated modulation of MHC-I and CD4 after sexual transmission of HIV-1

Categories:

Transfection

Authors:

Noviello CM, Kosakovsky Pond SL, Lewis MJ, Richman DD, Pillai SK, Yang OO, Little SJ, Smith DM, Guatelli JC

In:

J Virol (2007) 81(9): 4776-86

SUP-T1

Transfection Information

Citations