cells

B cell line

Cell Type:

B cell

Tissue Origin:

blood

Species:

chicken

Research Area:

Immunotherapy / Hematology

Cell Characteristics:

Suspension

Transfection Information

Lonza Optimized Protocol
Optimization Guideline

Filter:

The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.

Kit	Program	Cells	Efficiency	Viable Cells	Substrate		Format	Platform
T	K-001	1e6	25%	56%	Plasmid (general)	5 µg	100 µl	I/II/2b
T	L-001	1e6	42%	42%	Plasmid (general)	5 µg	100 µl	I/II/2b
T	A-030	1e6	42%	75%	Plasmid (general)	5 µg	100 µl	I/II/2b
T	B-009	1e6	67%	70%	Plasmid (general)	5 µg	100 µl	I/II/2b
T	A-023	5e6	30%	good	Plasmid (general)	µg	100 µl	I/II/2b
T	A-030	5e6	30%	good	Plasmid (general)	µg	100 µl	I/II/2b
T	B-009	5e6	80%	medium	Plasmid (general)	µg	100 µl	I/II/2b
T	B-023	5e6	80%	medium	Plasmid (general)	µg	100 µl	I/II/2b
T	B-023	3e6	60-70%		Plasmid (general)	30 µg	100 µl	I/II/2b

Citations (21)

The 9-1-1 DNA clamp is required for immunoglobulin gene conversion

Categories:

Transfection

Authors:

Saberi A, Nakahara M, Sale JE, Kikuchi K, Arakawa H, Buerstedde JM, Takeda S, Sonoda E

In:

Mol Cell Biol (2008) 28(19): 6113-22

ATP modulation of Ca2+ release by type-2 and type-3 InsP3R: Differing ATP sensitivities and molecular determinants of action

Categories:

Transfection

Authors:

Betzenhauser MJ, Wagner LE 2nd, Iwai M, Michikawa T, Mikoshiba K, Yule DI

In:

J Biol Chem (2008) 283(31): 21579-87

Molecular and Genetic Analysis of Condensin Function in Vertebrate Cells

Categories:

Transfection

Authors:

Hudson DF, Ohta S, Freisinger T, Macisaac F, Sennels L, Alves F, Lai F, Kerr A, Rappsilber J, Earnshaw WC

In:

Mol Biol Cell (2008) 19(7): 3070-3079

Phospholipase C-gamma-2 and Vav cooperate within signaling microclusters to propagate B cell spreading in response to membrane-bound antigen

Categories:

Transfection

Authors:

Weber M, Treanor B, Depoil D, Shinohara H, Harwood NE, Hikida M, Kurosaki T, Batista FD

In:

J Exp Med (2008) 205(4): 853-68

Molecular characterization of the Inositol 1,4,5-trisphosphate receptor pore-forming segment

Categories:

Transfection

Authors:

Schug ZT, da Fonseca PC, Bhanumathy CD, Wagner L 2nd, Zhang X, Bailey B, Morris EP, Yule DI, Joseph SK

In:

J Biol Chem (2008) 283(5): 2939-48

Phosphorylation of inositol 1,4,5-trisphosphate receptors by protein kinase B/Akt inhibits Ca2+ release and apoptosis

Categories:

Transfection

Authors:

Szado T, Vanderheyden V, Parys JB, De Smedt H, Rietdorf K, Kotelevets L, Chastre E, Khan F, Landegren U, Söderberg O, Bootman MD, Roderick HL

In:

Proc Natl Acad Sci USA (2008) 105(7): 2427-32

Apoptosis regulation by Bcl-xL modulation of mammalian inositol 1,4,5-trisphosphate receptor channel isoform gating

Categories:

Transfection

Authors:

Li C, Wang X, Vais H, Thompson CB, Foskett JK, White C

In:

Proc Natl Acad Sci USA (2007) 104(30): 12565-70

RAD18 and poly[ADP ribose]polymerase independently suppress the access of nonhomologous end joining to double strand breaks and facilitate homologous recombination mediated repair

Categories:

Transfection

Authors:

Saberi A, Hochegger H, Szuts D, Lan L, Yasui A, Sale JE, Taniguchi Y, Murakawa Y, Zheng W, Yokomori K, Helleday T, Teraoka H, Arakawa H, Buerstedde JM, Takeda S

In:

Mol Cell Biol (2007) 27(7): 2562-71

Transient transfection of DT40

Categories:

Transfection

Authors:

Franklin R, Sale JE

In:

Subcell Biochem (2006) 40: 379-82

A role for RAD18 in homologous recombination in DT40

Categories:

Transfection

Authors:

Szuts D, Simpson LJ, Kabani S, Yamazoe M, Sale JE

In:

Mol Cell Biol (2006) 26(21): 8032-41

ATP binding to a unique site in the type-1 S2- Inositol 1,4,5-trisphosphate receptor defines susceptibility to phosphorylation by protein kinase A

Categories:

Transfection

Authors:

Wagner LE, Betzenhauser MJ, Yule DI

In:

J Biol Chem (2006) 281(25): 17410-9

REV3 and REV1 play major roles in recombination-independent repair of DNA interstrand cross-links mediated by monoubiquitinated PCNA

Categories:

Transfection

Authors:

Shen X, Jun S, O'neal LE, Sonoda E, Bemark M, Sale JE, Li L

In:

J Biol Chem (2006) 281(20): 13869-72

The CENP-H-I complex is required for the efficient incorporation of newly synthesized CENP-A into centromeres

Categories:

Transfection

Authors:

Okada M, Cheeseman IM, Hori T, Okawa K, McLeod IX, Yates JR, Desai A, Fukagawa T

In:

Nat Cell Biol (2006) 8(5): 446-57

Parp-1 protects homologous recombination from interference by Ku and Ligase IV in vertebrate cells

Categories:

Transfection

Authors:

Hochegger H, Dejsuphong D, Fukushima T, Morrison C, Sonoda E, Schreiber V, Zhao GY, Saberi A, Masutani M, Adachi N, Koyama H, de Murcia G, Takeda S

In:

EMBO J (2006) 25(6): 1305-14

Phosphorylation of the CARMA1 linker controls NF-kappaB activation

Categories:

Transfection

Authors:

Sommer K, Guo B, Pomerantz JL, Bandaranayake AD, Moreno-Garcia ME, Ovechkina YL, Rawlings DJ

In:

Immunity (2005) 23(6): 561-74

The endoplasmic reticulum gateway to apoptosis by Bcl-X(L) modulation of the InsP(3)R

Categories:

Transfection

Authors:

White C, Li C, Yang J, Petrenko NB, Madesh M, Thompson CB and Foskett JK

In:

Nat Cell Biol (2005) 7(10): 1021-1028

The BRIP1 helicase functions independently of BRCA1 in the Fanconi anemia pathway for DNA crosslink repair

Categories:

Transfection

Authors:

Bridge WL, Vandenberg CJ, Franklin RJ and Hiom K

In:

Nat Genet (2005) 37(9): 953-957

Fen-1 facilitates homologous recombination by removing divergent sequences at DNA break ends

Categories:

Transfection

Authors:

Kikuchi K, Taniguchi Y, Hatanaka A, Sonoda E, Hochegger H, Adachi N, Matsuzaki Y, Koyama H, van Gent DC, Jasin M and Takeda S

In:

Mol Cell Biol (2005) 25(16): 6948-6955

Subtype-specific and ER lumenal environment-dependent regulation of inositol 1,4,5-trisphosphate receptor type 1 by ERp44

Categories:

Transfection

Authors:

Higo T, Hattori M, Nakamura T, Natsume T, Michikawa T and Mikoshiba K

In:

Cell (2005) 120(1): 85-98

Identification of protein tyrosine kinases required for B-cell- receptor-mediated activation of an Epstein-Barr Virus immediate-early gene promoter

Categories:

Transfection

Authors:

Lavens S, Faust EA, Lu F, Jacob M, Leta M, Lieberman PM and Pure E

In:

J Virol (2004) 78(16): 8543-8551

The Fanconi anaemia gene FANCC promotes homologous recombination and error-prone DNA repair

Categories:

Transfection

Authors:

Niedzwiedz W, Mosedale G, Johnson M, Ong CY, Pace P and Patel KJ

In:

Mol Cell (2004) 15(4): 607-620

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