CV1

african green monkey kidney

Cell Type:
Fibroblast
Tissue Origin:
kidney
Species:
monkey
Research Area:
Cancer Research/Cell Biology
Gastroenterology
Cell Characteristics:
Suspension

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
V A-033 1e6 70% 90% shRNA plasmid 5 µg 100 µl I/II/2b

Citations

Categories:
Transfection 
Authors:
Revenu C, Courtois M, Michelot A, Sykes C, Louvard D, Robine S 
In:
Mol Biol Cell (2007) 18(3): 827-838 
Categories:
Transfection 
Authors:
King ME, Kan HM, Baas PW, Erisir A, Glabe CG, Bloom GS 
In:
J Cell Biol (2006) 175(4): 541-6 
Categories:
Transfection 
Authors:
Preston CM, Nicholl MJ 
In:
J Gen Virol (2006) 87(Pt 5): 1113-21 
Categories:
Transfection 
Authors:
Muthumani K, Choo AY, Zong WX, Madesh M, Hwang DS, Premkumar A, Thieu KP, Emmanuel J, Kumar S, Thompson CB, Weiner DB 
In:
Nat Cell Biol (2006) 8(2): 170-9 
Categories:
Transfection 
Authors:
Tagawa A, Mezzacasa A, Hayer A, Longatti A, Pelkmans L and Helenius A 
In:
J Cell Biol (2005) 170(5): 769-779 
Categories:
Transfection 
Authors:
Vonderheit A, Helenius A 
In:
PLoS Biol (2005) 3(7): e233