Keratinocyte, (NHEK-Ad) human adult

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Primary keratinocytes; darkly & lightly pigmented, derived from human adult skin tissue.

Cell Type:
Skin/Hair Epith.
Tissue Origin:
dermal
Species:
human
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Research Area:
Cancer Research/Cell Biology
Dermatology/Tissue Engineering
Cell Characteristics:
Adherent

Recommended Media

Keratinocyte Growth Medium

Our first media specialised for growing keratinocytes.
Now superceded by KGM-Gold

Keratinocyte Growth Medium - Calcium-free

The original medium for growth of keratinocytes but without Calcium.

Keratinocyte Growth Medium 2

KGM-2 has been discontinued. As alternative we recommend the KGM-Gold BulletKit 00192060

Supports proliferation of primary human keratinocytes in culture.
Drives faster proliferation than the original KGM.
Now superceded by KGM-Gold

Keratinocyte Growth Medium 2, Calcium-free

A serum-free, calcium free keratinocyte growth medium

Keratinocyte Growth Medium Gold

Optimized for Normal Human Epidermal Keratinocytes in a serum-free environment.
Provides a nutrient rich medium that provides normal physiological growth characteristics and population doubling times.
KGM-Gold supports low, moderate, and high density keratinocyte cultures better than KGM or KGM-2, without nutrient depletion.

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
P3

96-DS-138

 1e5 60% 50-74% Plasmid (general) 0.4 µg 20 µl 96-well
Keratinocyte, human T-024 7e5 38-64% 40-60% Plasmid (general) 2.5 µg 100 µl I/II/2b
P3

DS-138

 1e5 60% 50-74% Plasmid (general) 0.4 µg 20 µl 4D X-Unit

Citations (24)

A role for parasite-induced PGE2 in IL-10-mediated host immunoregulation by skin stage schistosomula of Schistosoma mansoni
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Ramaswamy K, Kumar P, He YX. 
In:
J Immunol (2014) 165(8): 4567-74 
Down-regulation of the Antisense Mitochondrial ncRNAs is a Unique Vulnerability of Cancer Cells and a Potential Target for Cancer Therapy
Categories:
Primary Cells and Media 
Authors:
Vidaurre S1, Fitzpatrick C2, Burzio VA3, Briones M2, Villota C2, Villegas J2, Echenique J2, Oliveira-Cruz L2, Araya M2, Borgna V2, Socías T2, Lopez C2, Avila R2, Burzio LO2 
In:
J Biol Chem (2014) M114: 558841 
Histamine exerts multiple effects on expression of genes associated with epidermal barrier function
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Gutowska-Owsiak D, Salimi M, Selvakumar TA, Wang X, Taylor S, Ogg GS. 
In:
J Investig Allergol Clin Immunol (2014) 24(4): 231-9 
Epigallocatechin-3-O-(3-O-methyl)-gallate-induced differentiation of human keratinocytes involves klotho-mediated regulation of protein kinase-cAMP responsive element-binding protein signaling
Categories:
Primary Cells and Media 
Authors:
Kim HJ, Chang H, Han SH, Lee MS, Jung JY, An S, Baek SY, Lee JH, Lee JH, Lee TR, Shin DW, Kim H 
In:
Int J Mol Sci (2014) 15(4): 5749-61 
Lineage -CD34+CD31+ cells that appear in association with severe burn injury are inhibitory on the production of antimicrobial peptides by epidermal keratinocytes
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Yoshida S1, Lee JO2, Nakamura K1, Suzuki S3, Hendon DN2, Kobayashi M4, Suzuki F4. 
In:
PLoS ONE (2014) 9(2): e82926 
Desmosome assembly and disassembly are membrane raft-dependent.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Stahley SN, Saito M, Faundez V, Koval M, Mattheyses AL, Kowalczyk AP 
In:
PLoS ONE (2014) 9(1): e87809 
Senescent fibroblasts enhance early skin carcinogenic events via a paracrine MMP-PAR-1 axis.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Malaquin N1, Vercamer C, Bouali F, Martien S, Deruy E, Wernert N, Chwastyniak M, Pinet F, Abbadie C, Pourtier A. 
In:
PLoS ONE (2013) 8(5): e63607 
Influence of genistein on c-Jun, c-Fos and Fos-B of AP-1 subunits expression in skin keratinocytes, fibroblasts and keloid fibroblasts cultured in vitro
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Jurzak M, Adamczyk K 
In:
Acta Pol Pharm (2013) 70(2): 205-13 
Nitric oxide synthase gene transfer overcomes the inhibition of wound healing by sulfur mustard in a human keratinocyte in vitro model.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Ishida H1, Ray R, Amnuaysirikul J, Ishida K, Ray P. 
In:
International Scholarly Research Network (2012) 2012: 190429 
Diverse epigenetic strategies interact to control epidermal differentiation.
Categories:
Transfection 
Authors:
Mulder KW, Wang X, Escriu C, Ito Y, Schwarz RF, Gillis J, Sirokmány G, Donati G, Uribe-Lewis S, Pavlidis P, Murrell A, Markowetz F, Watt FM. 
In:
Nat Cell Biol (2012) 14(7): 753-63 
ZNF750 is expressed in differentiated keratinocytes and regulates epidermal late differentiation genes.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Cohen I1, Birnbaum RY, Leibson K, Taube R, Sivan S, Birk OS. 
In:
PLoS ONE (2012) 7(8): e42628 
Mapping and analysis of chromatin state dynamics in nine human cell types.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Ernst J, Kheradpour P, Mikkelsen TS, Shoresh N, Ward LD, Epstein CB, Zhang X, Wang L, Issner R, Coyne M, Ku M, Durham T, Kellis M, Bernstein BE 
In:
Nature (2011) 473(7345): 43-9 
Keratinocyte-derived anosmin-1, an extracellular glycoprotein encoded by the X-linked Kallmann syndrome gene, is involved in modulation of epidermal nerve density in atopic dermatitis
Categories:
Primary Cells and Media 
Authors:
Tengara S, Tominaga M, Kamo A, Taneda K, Negi O, Ogawa H, Takamori K. 
In:
J Dermatol Sci (2010) 58(1):: 64-71 
Evaluation of silver nanoparticle toxicity in skin in vivo and keratinocytes in vitro
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Samberg ME, Oldenburg SJ, Monteiro-Riviere NA. 
In:
Environ Health Perspect (2010) 118(3): 407-13 
Despite IFN-lambda receptor expression, blood immune cells, but not keratinocytes or melanocytes, have an impaired response to type III interferons: implications for therapeutic applications of these cytokines.
Categories:
Primary Cells and Media 
Authors:
Witte K, Gruetz G, Volk HD, Looman AC, Asadullah K, Sterry W, Sabat R, Wolk K. 
In:
Genes Immun (2009) 10(8): 702-714 
Reference gene selection for real-time rtPCR in human epidermal keratinocytes
Categories:
Transfection 
Authors:
Allen D, Winters E, Kenna PF, Humphries P, Jane Farrar G 
In:
J Dermatol Sci (2008) 49(3): 217-25 
Reciprocal regulation of p63 by C/EBPdelta in human keratinocytes
Categories:
Transfection 
Authors:
Borrelli S, Testoni B, Callari M, Alotto D, Castagnoli C, Romano RA, Sinha S, Vigano AM, Mantovani R 
In:
BMC Mol Biol (2007) 8(1): 85 
Tumor Necrosis Factor Receptor 1/c-Jun-NH2-Kinase Signaling Promotes Human Neoplasia
Categories:
Transfection 
Authors:
Zhang JY, Adams AE, Ridky TW, Tao S, Khavari PA 
In:
Cancer Res (2007) 67(8): 3827-34 
The protective role of a small GTPase RhoE against UVB induced DNA damage in keratinocytes
Categories:
Transfection 
Authors:
Boswell SA, Ongusaha PP, Nghiem P, Lee SW 
In:
J Biol Chem (2007) 282(7): 4850-8 
p63 regulates proliferation and differentiation of developmentally mature keratinocytes
Categories:
Transfection 
Authors:
Truong AB, Kretz M, Ridky TW, Kimmel R, Khavari PA 
In:
Genes Dev (2006) 20(22): 3185-97 
Oxidative inhibition of receptor type protein tyrosine phosphatase kappa by ultraviolet irradiation activates EGFR in human keratinocytes
Categories:
Transfection 
Authors:
Xu Y, Shao Y, Voorhees JJ, Fisher GJ 
In:
J Biol Chem (2006) 281(37): 27389-97 
Receptor-type Protein-tyrosine Phosphatase- Regulates Epidermal Growth Factor Receptor Function
Categories:
Transfection 
Authors:
Xu Y, Tan LJ, Grachtchouk V, Voorhees JJ and Fisher GJ 
In:
J Biol Chem (2005) 280(52): 42694-42700 
Nucleofection: a new, highly efficient transfection method for primary human keratinocytes
Categories:
Transfection 
Authors:
Distler JH, Jungel A, Kurowska-Stolarska M, Michel BA, Gay RE, Gay S and Distler O 
In:
Exp Dermatol (2005) 14(4): 315-320 
Downregulation of TGFbeta isoforms and their receptors contributes to keratinocyte hyperproliferation in psoriasis vulgaris
Categories:
Transfection 
Authors:
Doi H, Shibata MA, Kiyokane K and Otsuki Y 
In:
J Dermatol Sci (2003) 33: 7-16 
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