Keratinocyte, (NHEK-Ad) human adult

Primary keratinocytes; darkly & lightly pigmented, derived from human adult skin tissue.

Cell Type:
Skin/Hair Epith.
Tissue Origin:
dermal
Species:
human
Research Area:
Cancer Research/Cell Biology
Dermatology/Tissue Engineering
Cell Characteristics:
Adherent

Recommended Media

Our first media specialised for growing keratinocytes.
Now superceded by KGM-Gold

The original medium for growth of keratinocytes but without Calcium.

Supports proliferation of primary human keratinocytes in culture.
Drives faster proliferation than the original KGM.
Now superceded by KGM-Gold

A serum-free, calcium free keratinocyte growth medium

Optimized for Normal Human Epidermal Keratinocytes in a serum-free environment.
Provides a nutrient rich medium that provides normal physiological growth characteristics and population doubling times.
KGM-Gold supports low, moderate, and high density keratinocyte cultures better than KGM or KGM-2, without nutrient depletion.

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
P3

96-DS-138

1e5 60% 50-74% Plasmid (general) 0.4 µg 20 µl Shuttle
Keratinocyte, human T-024 7e5 38-64% 40-60% Plasmid (general) 2.5 µg 100 µl I/II/2b
P3

DS-138

1e5 60% 50-74% Plasmid (general) 0.4 µg 20 µl 4D X-Unit

Citations (22)

Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Ramaswamy K, Kumar P, He YX. 
In:
J Immunol (2014) 165(8): 4567-74 
Categories:
Primary Cells and Media 
Authors:
Vidaurre S1, Fitzpatrick C2, Burzio VA3, Briones M2, Villota C2, Villegas J2, Echenique J2, Oliveira-Cruz L2, Araya M2, Borgna V2, Socías T2, Lopez C2, Avila R2, Burzio LO2 
In:
J Biol Chem (2014) M114: 558841 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Gutowska-Owsiak D, Salimi M, Selvakumar TA, Wang X, Taylor S, Ogg GS. 
In:
J Investig Allergol Clin Immunol (2014) 24(4): 231-9 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Kim HJ, Chang H, Han SH, Lee MS, Jung JY, An S, Baek SY, Lee JH, Lee JH, Lee TR, Shin DW, Kim H 
In:
Int J Mol Sci (2014) 15(4): 5749-61 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Yoshida S1, Lee JO2, Nakamura K1, Suzuki S3, Hendon DN2, Kobayashi M4, Suzuki F4. 
In:
PLoS ONE (2014) 9(2): e82926 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Stahley SN, Saito M, Faundez V, Koval M, Mattheyses AL, Kowalczyk AP 
In:
PLoS ONE (2014) 9(1): e87809 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Malaquin N1, Vercamer C, Bouali F, Martien S, Deruy E, Wernert N, Chwastyniak M, Pinet F, Abbadie C, Pourtier A. 
In:
PLoS ONE (2013) 8(5): e63607 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Jurzak M, Adamczyk K 
In:
Acta Pol Pharm (2013) 70(2): 205-13 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Ishida H1, Ray R, Amnuaysirikul J, Ishida K, Ray P. 
In:
International Scholarly Research Network (2012) 2012: 190429 
Categories:
Transfection 
Authors:
Mulder KW, Wang X, Escriu C, Ito Y, Schwarz RF, Gillis J, Sirokmány G, Donati G, Uribe-Lewis S, Pavlidis P, Murrell A, Markowetz F, Watt FM. 
In:
Nat Cell Biol (2012) 14(7): 753-63 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Cohen I1, Birnbaum RY, Leibson K, Taube R, Sivan S, Birk OS. 
In:
PLoS ONE (2012) 7(8): e42628 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Ernst J, Kheradpour P, Mikkelsen TS, Shoresh N, Ward LD, Epstein CB, Zhang X, Wang L, Issner R, Coyne M, Ku M, Durham T, Kellis M, Bernstein BE 
In:
Nature (2011) 473(7345): 43-9 
Categories:
Primary Cells and Media 
Authors:
Tengara S, Tominaga M, Kamo A, Taneda K, Negi O, Ogawa H, Takamori K. 
In:
J Dermatol Sci (2010) 58(1):: 64-71 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Samberg ME, Oldenburg SJ, Monteiro-Riviere NA. 
In:
Environ Health Perspect (2010) 118(3): 407-13 
Categories:
Primary Cells and Media 
Authors:
Witte K, Gruetz G, Volk HD, Looman AC, Asadullah K, Sterry W, Sabat R, Wolk K. 
In:
Genes Immun (2009) 10(8): 702-714 
Categories:
Transfection 
Authors:
Allen D, Winters E, Kenna PF, Humphries P, Jane Farrar G 
In:
J Dermatol Sci (2008) 49(3): 217-25 
Categories:
Transfection 
Authors:
Borrelli S, Testoni B, Callari M, Alotto D, Castagnoli C, Romano RA, Sinha S, Vigano AM, Mantovani R 
In:
BMC Mol Biol (2007) 8(1): 85 
Categories:
Transfection 
Authors:
Boswell SA, Ongusaha PP, Nghiem P, Lee SW 
In:
J Biol Chem (2007) 282(7): 4850-8 
Categories:
Transfection 
Authors:
Truong AB, Kretz M, Ridky TW, Kimmel R, Khavari PA 
In:
Genes Dev (2006) 20(22): 3185-97 
Categories:
Transfection 
Authors:
Xu Y, Shao Y, Voorhees JJ, Fisher GJ 
In:
J Biol Chem (2006) 281(37): 27389-97 
Categories:
Transfection 
Authors:
Xu Y, Tan LJ, Grachtchouk V, Voorhees JJ and Fisher GJ 
In:
J Biol Chem (2005) 280(52): 42694-42700 
Categories:
Transfection 
Authors:
Distler JH, Jungel A, Kurowska-Stolarska M, Michel BA, Gay RE, Gay S and Distler O 
In:
Exp Dermatol (2005) 14(4): 315-320 
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