Chondrocyte, human (NHAC-kn)

Articular cartilage cells, obtained post mortem.
Chondrocytes produce and maintain extracellular cartilage matrix. Cartilage provides joint cushioning and facilitates joint articulation.

Cell Type:
Cartilage
Tissue Origin:
cartilage
Species:
human
Research Area:
Dermatology/Tissue Engineering
Cell Characteristics:
Adherent

Recommended Media

CGM Bulletkit has been developed for the expansion of human chondrocytes.

Media developed for the differentiation of chondrocytes

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
P3 96-ER-100 2e5 72-76% 72-96% Plasmid (general) 1 µg 20 µl Shuttle
Chondrocyte, human U-024 5e5 55-75% 60-70% Plasmid (general) 5 µg 100 µl I/II/2b
P3

ER-100

2e5 72-76% 72-96% Plasmid (general) 1 µg 20 µl 4D X-Unit

Citations (16)

Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Giavaresi G, Bondioli E, Melandri D, Giardino R, Tschon M, Torricelli P, Cenacchi G, Rotini R, Castagna A, Veronesi F, Pagani S, Fini M 
In:
BMC Musculoskelet Disord (2013) 14: 12 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Birukova AA, Lee S, Starosta V, Wu T, Ho T, Kim J, Berliner JA, Birukov KG. 
In:
PLoS ONE (2012) 1: e30957 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Schwager J, Hoeller U, Wolfram S, Richard N. 
In:
BMC Complemen Altern Med (2011) 11: 105 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Sun L, Wang X, Kaplan DL. 
In:
Biomaterials (2011) 32(24): 5581-9 
Categories:
Transfection 
Authors:
Haag J, Voigt R, Soeder S, Aigner T 
In:
Osteoarthritis Cartilage (2008) epub: online 
Categories:
Transfection 
Authors:
Dvir-Ginzberg M, Gagarina V, Lee EJ, Hall DJ 
In:
J Biol Chem (2008) epub: online 
Categories:
Transfection 
Authors:
Muddasani P, Norman JC, Ellman M, van Wijnen AJ, Im HJ 
In:
J Biol Chem (2007) 282(43): 31409-21 
Categories:
Transfection 
Authors:
Fukui N, Ikeda Y, Ohnuki T, Hikita A, Tanaka S, Yamane S, Suzuki R, Sandell LJ, Ochi T 
In:
J Biol Chem (2006) 281(37): 27229-41 
Categories:
Transfection 
Authors:
Reinhold MI, Kapadia RM, Liao Z and Naski MC 
In:
J Biol Chem (2006) 281(3): 1381-1388 
Categories:
Transfection 
Authors:
Cecil DL, Johnson K, Rediske J, Lotz M, Schmidt AM and Terkeltaub R 
In:
J Immunol (2005) 175(12): 8296-8302 
Categories:
Transfection 
Authors:
McNulty AL, Stabler TV, Vail TP, McDaniel GE, Kraus VB 
In:
Arthritis Rheum (2005) 52(9): 2676-85 
Categories:
Transfection 
Authors:
Loeser RF, Yammani RR, Carlson CS, Chen H, Cole A, Im HJ, Bursch LS, Yan SD 
In:
Arthritis Rheum (2005) 52(8): 2376-85 
Categories:
Transfection 
Authors:
Pulai JI, Chen H, Im HJ, Kumar S, Hanning C, Hegde PS and Loeser RF 
In:
J Immunol (2005) 174(9): 5781-5788 
Categories:
Transfection 
Authors:
Liu-Bryan R, Pritzker K, Firestein GS and Terkeltaub R 
In:
J Immunol (2005) 174(8): 5016-5023 
Categories:
Transfection 
Authors:
Gresch O, Engel FB, Nesic D, Tran TT, England HM, Hickman ES, Korner I, Gan L, Chen S, Castro-Obregon S, Hammermann R, Wolf J, Muller-Hartmann H, Nix M, Siebenkotten G, Kraus G and Lun K 
In:
Methods (2004) 33(2): 151-163 
Categories:
Transfection 
Authors:
Hamm A, Krott N, Breibach I, Blindt R and Bosserhoff AK 
In:
Tissue Eng (2002) 8(2): 235-245 
+ Show All