Oligodendrocyte, rat
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Glial cells that produce supportive myelin sheaths around CNS axons.
Cell Type:
Glial Cells
Tissue Origin:
brain
Species:
rat
Research Area:
Neurobiology
Cell Characteristics:
Adherent
Transfection Information
Lonza Optimized Protocol
Optimization Guideline
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The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols
or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our
optimization strategy
to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol
Kit
Program
Cells
Efficiency
Viable Cells
Substrate
Format
Platform
Glial, basic
O-017
5e6
40-48%
60%
Plasmid (general)
1 µg
100 µl
I/II/2b
P3
CA-138
2e5
10-24%
69-111%
Plasmid (general)
500 ng
20 µl
4D X-Unit
Citations (11)
Integrin-associated Lyn kinase promotes cell survival by suppressing acid sphingomyelinase activity
Categories:
Transfection
Authors:
Chudakova DA, Zeidan YH, Wheeler BW, Yu J, Novgorodov SA, Kindy MS, Hannun YA, Gudz TI
In:
J Biol Chem (2008) epub: online
Myosin Va controls oligodendrocyte morphogenesis and myelination
Categories:
Transfection
Authors:
Sloane JA, Vartanian TK
In:
J Neurosci (2007) 27(42): 11366-75
A crucial role for p57(Kip2) in the intracellular timer that controls oligodendrocyte differentiation
Categories:
Transfection
Authors:
Dugas JC, Ibrahim A, Barres BA
In:
J Neurosci (2007) 27(23): 6185-96
Activation of sphingosine-1-phosphate receptor S1P5 inhibits oligodendrocyte progenitor migration
Categories:
Transfection
Authors:
Novgorodov AS, El-Alwani M, Bielawski J, Obeid LM, Gudz TI
In:
FASEB J (2007) 21(7): 1503-14
Identification of dystroglycan as a second laminin receptor in oligodendrocytes, with a role in myelination
Categories:
Transfection
Authors:
Colognato H, Galvin J, Wang Z, Relucio J, Nguyen T, Harrison D, Yurchenco PD, Ffrench-Constant C
In:
Development (2007) 134(9): 1723-36
Functional genomic analysis of oligodendrocyte differentiation
Categories:
Transfection
Authors:
Dugas JC, Tai YC, Speed TP, Ngai J, Barres BA
In:
J Neurosci (2006) 26(43): 10967-83
CNS progenitor cells and oligodendrocytes are targets of chemotherapeutic agents in vitro and in vivo
Categories:
Primary Cells and Media
Authors:
Dietrich J, Han R, Yang Y, Mayer-Pröschel M, Noble M.
In:
J Cell Biol (2006) 5(7): 22
Ermin, a myelinating oligodendrocyte-specific protein that regulates cell morphology
Categories:
Transfection
Authors:
Brockschnieder D, Sabanay H, Riethmacher D and Peles E
In:
J Neurosci (2006) 26(3): 757-762
Expression of stathmin, a developmentally controlled cytoskeleton-regulating molecule, in demyelinating disorders
Categories:
Transfection
Authors:
Liu A, Stadelmann C, Moscarello M, Bruck W, Sobel A, Mastronardi FG and Casaccia-Bonnefil P
In:
J Neurosci (2005) 25(3): 737-747
Chromatin remodeling and histone modification in the conversion of oligodendrocyte precursors to neural stem cells
Categories:
Transfection
Authors:
Kondo T and Raff M
In:
Genes Dev (2004) 18(23): 2963-2972
Integrins direct Src family kinases to regulate distinct phases of oligodendrocyte development
Categories:
Transfection
Authors:
Colognato H, Ramachandrappa S, Olsen M and ffrench-Constant C
In:
J Cell Biol (2004) 167(2): 365-375
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