Chromatin remodeling and histone modification in the conversion of oligodendrocyte precursors to neural stem cells

Authors:
Kondo T and Raff M
In:
Source: Genes Dev
Publication Date: (2004)
Issue: 18(23): 2963-2972
Research Area:
Neurobiology
Cells used in publication:
Oligodendrocyte, rat
Species: rat
Tissue Origin: brain
Platform:
Nucleofector® I/II/2b
Abstract
We showed previously that purified rat oligodendrocyte precursor cells (OPCs) can be induced by extracellular signals to convert to multipotent neural stem-like cells (NSLCs), which can then generate both neurons and glial cells. Because the conversion of precursor cells to stem-like cells is of both intellectual and practical interest, it is important to understand its molecular basis. We show here that the conversion of OPCs to NSLCs depends on the reactivation of the sox2 gene, which in turn depends on the recruitment of the tumor suppressor protein Brca1 and the chromatin-remodeling protein Brahma (Brm) to an enhancer in the sox2 promoter. Moreover, we show that the conversion is associated with the modification of Lys 4 and Lys 9 of histone H3 at the same enhancer. Our findings suggest that the conversion of OPCs to NSLCs depends on progressive chromatin remodeling, mediated in part by Brca1 and Brm.