Neuron, hippocampal, mouse

Nerve cells isolated from mouse hippocampus.

Cell Type:
Neurons (Brain)
Tissue Origin:
brain
Species:
mouse
Research Area:
Neurobiology
Cell Characteristics:
Adherent

Recommended Media

Primary Neuron Growth Medium Kit (PNGM™) was specifically developed for culturing embryonic primary rat and mouse neurons. This serum-free medium allows for long-term maintenance and growth of neuronal cells, and maintains pure populations of neuronal cells without the need of an astrocyte feeder layer.

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
Neuron, mouse O-005 4e6 39-77% Plasmid (general) 3 µg 100 µl I/II/2b
AD1 ED-158 8e4 90% 90% Plasmid (general) 18 µg 350 µl 4D Y-Unit

Citations (24)

Categories:
Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Iqbal Z1, Willemsen MH1, Papon MA2, Musante L3, Benevento M4, Hu H3, Venselaar H5, Wissink-Lindhout WM1, Vulto-van Silfhout AT1, Vissers LE1, de Brouwer AP6, Marouillat S2, Wienker TF3, Ropers HH3, Kahrizi K7, Nadif Kasri N6, Najmabadi H7, Laumonnier F8, Kleefstra T1, van Bokhoven H9. 
In:
Am J Hum Genet (2015) 96(3): 386-396 
Categories:
Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Pomponio G, Zurich MG, Schultz L, Weiss DG, Romanelli L, Gramowski-Voss A, Di Consiglio E, Testai E. 
In:
Toxicol In Vitro (2015) (15)00013-2: 1 
Categories:
Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Labonté D, Thies E, Kneussel M. 
In:
Eur J Cell Biol (2014) 93(8-9): 338-346 
Categories:
Transfection 
Authors:
Sia GM, Clem RL, Huganir RL. 
In:
Science (2013) 342: 987-991 
Categories:
Transfection 
Authors:
Choi YJ, Di Nardo A, Kramvis I, Meikle L, Kwiatkowski DJ, Sahin M, He X 
In:
Genes Dev (2008) 22(18): 2485-95 
Categories:
Transfection 
Authors:
Pandithage R, Lilischkis R, Harting K, Wolf A, Jedamzik B, Lüscher-Firzlaff J, Vervoorts J, Lasonder E, Kremmer E, Knöll B, Lüscher B 
In:
J Cell Biol (2008) 180(5): 915-29 
Categories:
Transfection 
Authors:
Schneider A, Rajendran L, Honsho M, Gralle M, Donnert G, Wouters F, Hell SW, Simons M 
In:
J Neurosci (2008) 28(11): 2874-82 
Categories:
Transfection 
Authors:
Calderon de Anda F, Gärtner A, Tsai LH, Dotti CG 
In:
J Cell Sci (2008) 121(Pt 2): 178-85 
Categories:
Transfection 
Authors:
Garvalov BK, Flynn KC, Neukirchen D, Meyn L, Teusch N, Wu X, Brakebusch C, Bamburg JR, Bradke F 
In:
J Neurosci (2007) 27(48): 13117-29 
Categories:
Transfection 
Authors:
Zeitelhofer M, Vessey JP, Xie Y, Tübing F, Thomas S, Kiebler M, Dahm R 
In:
Nat Protocols (2007) 2(7): 1692-704 
Categories:
Transfection 
Authors:
Kim WY, Zhou FQ, Zhou J, Yokota Y, Wang YM, Yoshimura T, Kaibuchi K, Woodgett JR, Anton ES, Snider WD 
In:
Neuron (2006) 52(6): 981-96 
Categories:
Transfection 
Authors:
Gaertner A, Huang X, Hall A 
In:
J Cell Sci (2006) 119(Pt 19): 3927-34 
Categories:
Transfection 
Authors:
Ninan I, Liu S, Rabinowitz D, Arancio O 
In:
EMBO J (2006) 25(18): 4361-71 
Categories:
Transfection 
Authors:
Togashi H, Miyoshi J, Honda T, Sakisaka T, Takai Y, Takeichi M 
In:
J Cell Biol (2006) 174(1): 141-51 
Categories:
Transfection 
Authors:
Knoell B, Kretz O, Fiedler C, Alberti S, Schutz G, Frotscher M, Nordheim A 
In:
Nat Neurosci (2006) 9(2): 195-204 
Categories:
Transfection 
Authors:
Kholmanskikh SS, Koeller HB, Wynshaw-Boris A, Gomez T, Letourneau PC, Ross ME 
In:
Nat Neurosci (2006) 9(1): 50-7 
Categories:
Transfection 
Authors:
Yang T, Yin W, Derevyanny VD, Moore LA and Longo FM 
In:
Eur J Neurosci (2005) 22(9): 2159-2170 
Categories:
Transfection 
Authors:
Lie DC, Colamarino SA, Song HJ, Desire L, Mira H, Consiglio A, Lein ES, Jessberger S, Lansford H, Dearie AR, Gage FH 
In:
Nature (2005) 437(7063): 1370-5 
Categories:
Transfection 
Authors:
Cheng L, Lemmon S and Lemmon V 
In:
J Neurochem (2005) 94(4): 1102-1110 
Categories:
Transfection 
Authors:
Kvachnina E, Liu G, Dityatev A, Renner U, Dumuis A, Richter DW, Dityateva G, Schachner M, Voyno-Yasenetskaya TA and Ponimaskin EG 
In:
J Neurosci (2005) 25(34): 7821-7830 
Categories:
Transfection 
Authors:
Paganoni S and Ferreira A 
In:
J Cell Sci (2005) 118(Pt 2): 433-446 
Categories:
Transfection 
Authors:
Nikolaev VO, Bunemann M, Hein L, Hannawacker A and Lohse MJ 
In:
J Biol Chem (2004) 279(36): 37215-8 
Categories:
Transfection 
Authors:
Obermair GJ, Szabo Z, Bourinet E and Flucher BE 
In:
Eur J Neurosci (2004) 19(8): 2109-2122 
Categories:
Transfection 
Authors:
Dityateva G, Hammond M, Thiel C, Ruonala MO, Delling M, Siebenkotten G, Nix M and Dityatev A 
In:
J Neurosci Methods (2003) 130(1): 65-73 
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