Astrocyte, rat

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Star-shaped glial cells that provide nutrients, support, and insulation for neurons of the central nervous system.

Cell Type:
Glial Cells
Tissue Origin:
brain
Species:
rat
Research Area:
Cancer Research/Cell Biology
Neurobiology
Cell Characteristics:
Adherent

Recommended Media

Astrocyte Growth Medium

Astrocyte Growth Medium (AGM™) has been developed to support the growth of primary human astrocytes.

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
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The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
Glial, basic T-020 2e6 54-64% 70-80% Plasmid (general) 5 µg 100 µl I/II/2b

Citations (21)

Translating intracarotid artery transplantation of bone marrow-derived NCS-01 cells for ischemic stroke: Behavioral and histological readouts and mechanistic insights into stem cell therapy.
Categories:
Primary Cells and Media 
Authors:
Kaneko Y, Lee JY, Tajiri N, Tuazon JP, Lippert T, Russo E, Yu SJ, Bonsack B, Corey S, Coats AB, Kingsbury C, Chase TN, Koga M, Borlongan CV 
In:
STEM CELLS TRANSLATIONALMEDICINE (2019) 10: 1002 
Validation of Endothelin B Receptor Antibodies Reveals Two Distinct Receptor-related Bands on Western Blot.
Categories:
Primary Cells and Media 
Authors:
Barr TP, Kornberg D, Montmayeur JP, Long M, Reichheld S, Strichartz GR. 
In:
Anal Biochem (2015) 1: 468:28-33 
RIG-I contributes to the innate immune response after cerebral ischemia
Categories:
Primary Cells and Media 
Authors:
Brand FJ 3rd, de Rivero Vaccari JC, Mejias NH, Alonso OF, de Rivero Vaccari JP. 
In:
J Inflamm (2015) 12(52): 1 
Biased mGlu5-Positive Allosteric Modulators Provide In Vivo Efficacy without Potentiating mGlu5 Modulation of NMDAR Currents.
Categories:
Primary Cells and Media 
Authors:
Rook JM, Xiang Z, Lv X, Ghoshal A, Dickerson JW, Bridges TM, Johnson KA, Foster DJ, Gregory KJ, Vinson PN, Thompson AD, Byun N, Collier RL, Bubser M, Nedelcovych MT, Gould RW, Stauffer SR, Daniels JS, Niswender CM, Lavreysen H, Mackie C, Conde-Ceide S, Alcazar J, Bartolomé-Nebreda JM, Macdonald GJ, Talpos JC, Steckler T, Jones CK, Lindsley CW, Conn PJ 
In:
Neuron (2015) 86(4): 1029-40 
Fast and Efficient Neural Conversion of Human Hematopoietic Cells
Categories:
Primary Cells and Media 
Authors:
Castaño J, Menendez P, Bruzos-Cidon C, Straccia M, Sousa A5, Zabaleta L, Vazquez N, Zubiarrain A, Sonntag KC, Ugedo L, Carvajal-Vergara X, Canals JM, Torrecilla M, Sanchez-Pernaute R, Giorgetti A 
In:
Stem Cell Reports (2014) ePub: S2213-6711 
Interpretation of Cellular Imaging and AQP4 Quantification Data in a Single Cell Simulator
Categories:
Primary Cells and Media 
Authors:
Kim SB, Hsu Y, Linninger AA 
In:
Other (2014) 2(1): 218-237 
Drug release from porous silicon for stable neural interface
Categories:
Primary Cells and Media 
Authors:
Sun T, Tsang WM, Park WT 
In:
Other (2014) 292: 843-851 
ROS detoxification and proinflammatory cytokines are linked by p38 MAPK signaling in a model of mature astrocyte activation.
Categories:
Primary Cells and Media 
Authors:
Nahirnyj A, Livne-Bar I, Guo X, Sivak J. 
In:
PLoS ONE (2013) 8(12): e83049. 
A novel metabotropic glutamate receptor 5 positive allosteric modulator acts at a unique site and confers stimulus bias to mGlu5 signaling.
Categories:
Primary Cells and Media 
Authors:
Noetzel MJ, Gregory KJ, Vinson PN, Manka JT, Stauffer SR, Lindsley CW, Niswender CM, Xiang Z, Conn PJ 
In:
Mol Pharmacol (2013) 83(4): 835-47 
Critical role of the a1-Na+, K +-ATPase subunit in insensitivity of rodent cells to cytotoxic action of ouabain.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Akimova OA, Tverskoi AM, Smolyaninova LV, Mongin AA, Lopina OD, La J, Dulin NO, Orlov SN 
In:
Apoptosis (2012) ePub: e 
Optimization of endothelial cell growth in a murine in vitro blood-brain barrier model
Categories:
Primary Cells and Media 
Authors:
Wuest DM, Lee KH 
In:
Biotechnology Journal (2012) 7(3): 409-17 
Attenuation of scratch-induced reactive astrogliosis by novel EphA4 kinase inhibitors
Categories:
Primary Cells and Media 
Authors:
Parmentier-Batteur S, Finger EN, Krishnan R, Rajapakse HA, Sanders JM, Kandpal G, Zhu H, Moore KP, Regan CP, Sharma S, Hess JF, Williams TM, Reynolds IJ, Vacca JP, Mark RJ, Nantermet PG 
In:
J Neurochem (2011) 118(6): 1016-31 
Pharmacological assessment of ARTCEREB irrigation and perfusion solution for cerebrospinal surgery using primary cultures of rat brain cells.
Categories:
Primary Cells and Media 
Authors:
Nishimura M, Doi K, Kishimoto S, Koshitani O, Naito S, Yamauchi A. 
In:
J Toxicol Sci (2010) 35(4): 447-57 
Therapeutic targets and limits of minocycline neuroprotection in experimental ischemic stroke.
Categories:
Primary Cells and Media 
Authors:
Matsukawa N, Yasuhara T, Hara K, Xu L, Maki M, Yu G, Kaneko Y, Ojika K, Hess DC, Borlongan CV 
In:
BMC Neurosci (2009) 10: 126 
The brain-specific factor FEZ1 is a determinant of neuronal susceptibility to HIV-1 infection
Categories:
Primary Cells and Media 
Authors:
Haedicke J, Brown C, Naghavi MH. 
In:
Proc Natl Acad Sci USA (2009) 106(33): 14040-5 
Redefining the role of metallothionein within the injured brain: Extracellular metallothioneins play an important role in the astrocyte-neuron response to injury
Categories:
Transfection 
Authors:
Chung RS, Penkowa M, Dittmann J, King CE, Bartlett C, Asmussen JW, Hidalgo J, Carrasco J, Leung YK, Walker AK, Fung SJ, Dunlop SA, Fitzgerald M, Beazley LD, Chuah MI, Vickers JC, West AK 
In:
J Biol Chem (2008) 283(22): 15349-58 
Scrib Controls Cdc42 Localization and Activity to Promote Cell Polarization during Astrocyte Migration
Categories:
Transfection 
Authors:
Osmani N, Vitale N, Borg JP, Etienne-Manneville S 
In:
Curr Biol (2006) 16(24): 2395-405 
Epidermal growth factor receptor activation: an upstream signal for transition of quiescent astrocytes into reactive astrocytes after neural injury
Categories:
Transfection 
Authors:
Liu B, Chen H, Johns TG, Neufeld AH 
In:
J Neurosci (2006) 26(28): 7532-40 
Ermin, a myelinating oligodendrocyte-specific protein that regulates cell morphology
Categories:
Transfection 
Authors:
Brockschnieder D, Sabanay H, Riethmacher D and Peles E 
In:
J Neurosci (2006) 26(3): 757-762 
Differential promotion of glutamate transporter expression and function by glucocorticoids in astrocytes from various brain regions
Categories:
Transfection 
Authors:
Zschocke J, Bayatti N, Clement AM, Witan H, Figiel M, Engele J and Behl C 
In:
J Biol Chem (2005) 280(41): 34924-34932 
Cdc42 and Par6-PKC regulate the spatially localized association of Dlg1 and APC to control cell polarization
Categories:
Transfection 
Authors:
Etienne-Manneville S, Manneville JB, Nicholls S, Ferenczi MA and Hall A 
In:
J Cell Biol (2005) 170(6): 895-901 
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