MM.1S

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Multiple myeloma cell line. Dexamethasone sensitive subclone of MM.1.

Cell Type:
B cell
Tissue Origin:
blood
Species:
human
Research Area:
Cancer Research/Cell Biology
Immunotherapy / Hematology
Cell Characteristics:
Suspension

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
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The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
V O-020 2e6 40% 60% Plasmid (general) 2 µg 100 µl I/II/2b
V O-023 2e6 41% 63% Plasmid (general) 2 µg 100 µl I/II/2b
V S-020 2e6 50% 50% Plasmid (general) 2 µg 100 µl I/II/2b

Citations (18)

HDAC inhibitor AR-42 decreases CD44 expression and sensitizes myeloma cells to lenalidomide.
Categories:
Transfection, Cell Lines and Primary Cancer Cells 
Authors:
Canella A, Cordero Nieves H, Sborov DW, Cascione L, Radomska HS, Smith E, Stiff A, Consiglio J, Caserta E, Rizzotto L, Zanesi N, Stefano V, Kaur B, Mo X, Byrd JC, Efebera YA, Hofmeister CC, Pichiorri F. 
In:
OncoTarget (2015) 6(31): 31134-50 
Multiple Myeloma Cell Killing by Depletion of the MET Receptor Tyrosine Kinase
Categories:
Transfection 
Authors:
Stellrecht CM, Phillip CJ, Cervantes-Gomez F, Gandhi V 
In:
Cancer Res (2007) 67(20): 9913-20 
Up-Regulation of c-Jun Inhibits Proliferation and Induces Apoptosis via Caspase-Triggered c-Abl Cleavage in Human Multiple Myeloma
Categories:
Transfection 
Authors:
Podar K, Raab MS, Tonon G, Sattler M, Barila D, Zhang J, Tai YT, Yasui H, Raje N, Depinho RA, Hideshima T, Chauhan D, Anderson KC 
In:
Cancer Res (2007) 67(4): 1680-8 
The small-molecule VEGF receptor inhibitor pazopanib (GW786034B) targets both tumor and endothelial cells in multiple myeloma
Categories:
Transfection 
Authors:
Podar K, Tonon G, Sattler M, Tai YT, Legouill S, Yasui H, Ishitsuka K, Kumar S, Kumar R, Pandite LN, Hideshima T, Chauhan D, Anderson KC 
In:
Proc Natl Acad Sci USA (2006) 103(51): 19478-83 
Glucocorticoid receptor transcriptional isoforms and resistance in multiple myeloma cells
Categories:
Transfection 
Authors:
Sanchez-Vega B, Krett N, Rosen ST, Gandhi V 
In:
Mol Cancer Ther (2006) 5(12): 3062-70 
Perifosine, an oral bioactive novel alkylphospholipid, inhibits Akt and induces in vitro and in vivo cytotoxicity in human multiple myeloma cells
Categories:
Transfection 
Authors:
Hideshima T, Catley L, Yasui H, Ishitsuka K, Raje N, Mitsiades C, Podar K, Munshi NC, Chauhan D, Richardson PG and Anderson KC 
In:
Blood (2006) 107(10): 4053-62 
A novel orally active proteasome inhibitor induces apoptosis in multiple myeloma cells with mechanisms distinct from Bortezomib
Categories:
Transfection 
Authors:
Chauhan D, Catley L, Li G, Podar K, Hideshima T, Velankar M, Mitsiades C, Mitsiades N, Yasui H, Letai A, Ovaa H, Berkers C, Nicholson B, Chao TH, Neuteboom ST, Richardson P, Palladino MA and Anderson KC 
In:
Cancer Cell (2005) 8(5): 407-419 
Small-molecule inhibition of proteasome and aggresome function induces synergistic antitumor activity in multiple myeloma
Categories:
Transfection 
Authors:
Hideshima T, Bradner JE, Wong J, Chauhan D, Richardson P, Schreiber SL and Anderson KC 
In:
Proc Natl Acad Sci USA (2005) 102(24): 8567-8572 
VEGF induces MCL-1 upregulation and protects multiple myeloma cells against apoptosis
Categories:
Transfection 
Authors:
Le Gouill S, Podar K, Amiot M, Hideshima T, Chauhan D, Itshitsuka K, Kumar S, Raje N, Richardson PG, Harousseau JL and Anderson KC 
In:
Blood (2004) 104(9): 2886-2892 
p38 MAPK inhibition enhances PS-341 (bortezomib)-induced cytotoxicity against multiple myeloma cells
Categories:
Transfection 
Authors:
Hideshima T, Podar K, Chauhan D, Ishitsuka K, Mitsiades C, Tai YT, Hamasaki M, Raje N, Hideshima H, Schreiner G, Nguyen AN, Navas T, Munshi NC, Richardson PG, Higgins LS and Anderson KC 
In:
Oncogene (2004) 23(54): 8766-8776 
Targeting mitochondria to overcome conventional and bortezomib/proteasome inhibitor PS-341 resistance in multiple myeloma (MM) cells
Categories:
Transfection 
Authors:
Chauhan D, Li G, Podar K, Hideshima T, Mitsiades C, Schlossman R, Munshi N, Richardson P, Cotter FE and Anderson KC 
In:
Blood (2004) 104(8): 2458-2466 
Caveolin-1 is required for vascular endothelial growth factor-triggered multiple myeloma cell migration and is targeted by bortezomib
Categories:
Transfection 
Authors:
Podar K, Shringarpure R, Tai YT, Simoncini M, Sattler M, Ishitsuka K, Richardson PG, Hideshima T, Chauhan D and Anderson KC 
In:
Cancer Res (2004) 64(20): 7500-7506 
Critical Role for Hematopoietic Cell Kinase (Hck)-mediated Phosphorylation of Gab1 and Gab2 Docking Proteins in Interleukin 6-induced Proliferation and Survival of Multiple Myeloma Cells.
Categories:
Transfection 
Authors:
Podar K, Mostoslavsky G, Sattler M, Tai YT, Hayashi T, Catley LP, Hideshima T, Mulligan RC, Chauhan D and Anderson KC. 
In:
J Biol Chem (2004) 279(20): 21658-21665 
Blockade of ubiquitin-conjugating enzyme CDC34 enhances anti-myeloma activity of Bortezomib/Proteasome inhibitor PS-341
Categories:
Transfection 
Authors:
Chauhan D, Li G, Hideshima T, Podar K, Shringarpure R, Mitsiades C, Munshi N, Yew PR and Anderson KC 
In:
Oncogene (2004) 23(20): 3597-3602 
Antitumor activity of lysophosphatidic acid acyltransferase-beta inhibitors, a novel class of agents, in multiple myeloma
Categories:
Transfection 
Authors:
Hideshima T, Chauhan D, Hayashi T, Podar K, Akiyama M, Mitsiades C, Mitsiades N, Gong B, Bonham L, de Vries P, Munshi N, Richardson PG, Singer JW and Anderson KC 
In:
Cancer Res (2003) 63: 8428-8436 
HSP27 inhibits release of mitochondrial protein Smac in multiple myeloma cells and confers dexamethasone resistance
Categories:
Transfection 
Authors:
Chauhan D, Li G, Hideshima T, Podar K, Mitsiades C, Midsiades N, Catley L, Tai YT, Hayashi T, Shringharpure R, Burger R, Munshi N, Ohtake Y, Saxena S and Anderson KC 
In:
Blood (2003) 102(9): 3379-3386 
JNK-dependent release of mitochondrial protein, SMAC during apoptosis in multiple myeloma (MM) cells
Categories:
Transfection 
Authors:
Chauhan D, Li G, Hideshima T, Podar K, Mitsiades C, Midsiades N, Munshi N, Kharbanda S and Anderson KC 
In:
J Biol Chem (2003) 278(20): 17593-17596 
Identification of genes regulated by 2-methoxyestradiol (2ME2) in multiple myeloma cells using oligonucleotide arrays
Categories:
Transfection 
Authors:
Chauhan D, Li G, Auclair D, Hideshima T, Richardson P, Podar K, Mitsiades N, Mitsiades C, Li C, Kim RS, Munshi N, Chen LB, Wong W and Anderson KC 
In:
Blood (2003) 101(9): 3606-3614 
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