MM.1S

multiple myeloma cell line

Cell Type:
B cell
Tissue Origin:
blood
Species:
human
Research Area:
Cancer Research/Cell Biology
Immunotherapy / Hematology
Cell Characteristics:
Suspension

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
V O-020 2e6 40% 60% Plasmid (general) 2 µg 100 µl I/II/2b
V O-023 2e6 41% 63% Plasmid (general) 2 µg 100 µl I/II/2b
V S-020 2e6 50% 50% Plasmid (general) 2 µg 100 µl I/II/2b

Citations (17)

Categories:
Transfection 
Authors:
Stellrecht CM, Phillip CJ, Cervantes-Gomez F, Gandhi V 
In:
Cancer Res (2007) 67(20): 9913-20 
Categories:
Transfection 
Authors:
Podar K, Raab MS, Tonon G, Sattler M, Barila D, Zhang J, Tai YT, Yasui H, Raje N, Depinho RA, Hideshima T, Chauhan D, Anderson KC 
In:
Cancer Res (2007) 67(4): 1680-8 
Categories:
Transfection 
Authors:
Podar K, Tonon G, Sattler M, Tai YT, Legouill S, Yasui H, Ishitsuka K, Kumar S, Kumar R, Pandite LN, Hideshima T, Chauhan D, Anderson KC 
In:
Proc Natl Acad Sci USA (2006) 103(51): 19478-83 
Categories:
Transfection 
Authors:
Sanchez-Vega B, Krett N, Rosen ST, Gandhi V 
In:
Mol Cancer Ther (2006) 5(12): 3062-70 
Categories:
Transfection 
Authors:
Hideshima T, Catley L, Yasui H, Ishitsuka K, Raje N, Mitsiades C, Podar K, Munshi NC, Chauhan D, Richardson PG and Anderson KC 
In:
Blood (2006) 107(10): 4053-62 
Categories:
Transfection 
Authors:
Chauhan D, Catley L, Li G, Podar K, Hideshima T, Velankar M, Mitsiades C, Mitsiades N, Yasui H, Letai A, Ovaa H, Berkers C, Nicholson B, Chao TH, Neuteboom ST, Richardson P, Palladino MA and Anderson KC 
In:
Cancer Cell (2005) 8(5): 407-419 
Categories:
Transfection 
Authors:
Hideshima T, Bradner JE, Wong J, Chauhan D, Richardson P, Schreiber SL and Anderson KC 
In:
Proc Natl Acad Sci USA (2005) 102(24): 8567-8572 
Categories:
Transfection 
Authors:
Le Gouill S, Podar K, Amiot M, Hideshima T, Chauhan D, Itshitsuka K, Kumar S, Raje N, Richardson PG, Harousseau JL and Anderson KC 
In:
Blood (2004) 104(9): 2886-2892 
Categories:
Transfection 
Authors:
Hideshima T, Podar K, Chauhan D, Ishitsuka K, Mitsiades C, Tai YT, Hamasaki M, Raje N, Hideshima H, Schreiner G, Nguyen AN, Navas T, Munshi NC, Richardson PG, Higgins LS and Anderson KC 
In:
Oncogene (2004) 23(54): 8766-8776 
Categories:
Transfection 
Authors:
Chauhan D, Li G, Podar K, Hideshima T, Mitsiades C, Schlossman R, Munshi N, Richardson P, Cotter FE and Anderson KC 
In:
Blood (2004) 104(8): 2458-2466 
Categories:
Transfection 
Authors:
Podar K, Shringarpure R, Tai YT, Simoncini M, Sattler M, Ishitsuka K, Richardson PG, Hideshima T, Chauhan D and Anderson KC 
In:
Cancer Res (2004) 64(20): 7500-7506 
Categories:
Transfection 
Authors:
Podar K, Mostoslavsky G, Sattler M, Tai YT, Hayashi T, Catley LP, Hideshima T, Mulligan RC, Chauhan D and Anderson KC. 
In:
J Biol Chem (2004) 279(20): 21658-21665 
Categories:
Transfection 
Authors:
Chauhan D, Li G, Hideshima T, Podar K, Shringarpure R, Mitsiades C, Munshi N, Yew PR and Anderson KC 
In:
Oncogene (2004) 23(20): 3597-3602 
Categories:
Transfection 
Authors:
Hideshima T, Chauhan D, Hayashi T, Podar K, Akiyama M, Mitsiades C, Mitsiades N, Gong B, Bonham L, de Vries P, Munshi N, Richardson PG, Singer JW and Anderson KC 
In:
Cancer Res (2003) 63: 8428-8436 
Categories:
Transfection 
Authors:
Chauhan D, Li G, Hideshima T, Podar K, Mitsiades C, Midsiades N, Catley L, Tai YT, Hayashi T, Shringharpure R, Burger R, Munshi N, Ohtake Y, Saxena S and Anderson KC 
In:
Blood (2003) 102(9): 3379-3386 
Categories:
Transfection 
Authors:
Chauhan D, Li G, Hideshima T, Podar K, Mitsiades C, Midsiades N, Munshi N, Kharbanda S and Anderson KC 
In:
J Biol Chem (2003) 278(20): 17593-17596 
Categories:
Transfection 
Authors:
Chauhan D, Li G, Auclair D, Hideshima T, Richardson P, Podar K, Mitsiades N, Mitsiades C, Li C, Kim RS, Munshi N, Chen LB, Wong W and Anderson KC 
In:
Blood (2003) 101(9): 3606-3614 
+ Show All