cells

Plasmodium knowlesi is a primate malaria parasite commonly found in Southeast Asia. It causes malaria in long-tailed macaques (Macaca fascicularis), but it also infects humans.

Cell Type:

Plasmodium

Tissue Origin:

Species:

baboon

Research Area:

Parasitology

Cell Characteristics:

Suspension

Transfection Information

Lonza Optimized Protocol
Optimization Guideline

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The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.

Protocol	Kit	Program	Cells	Efficiency	Viable Cells	Substrate		Format	Platform
	P3	FP-158		30%		Plasmid (general)	20 µg	100 µl	4D X-Unit

Citations

Merozoite surface protein 1 paralog is involved in the human erythrocyte invasion of a zoonotic malaria, Plasmodium knowlesi

Categories:

Transfection

Authors:

Seong-Kyun Lee, Tuyet Kha Nguyen, Franziska Mohring, Jin-Hee Han, Egy Rahman Firdaus, Sung-Hun Na, Won-Sun Park, Robert W. Moon, Eun-Taek Han

In:

(2023) :

SICA-mediated cytoadhesion of Plasmodium knowlesi-infected red blood cells to human umbilical vein endothelial cells

Categories:

Transfection

Authors:

Huai Chuang, Miako Sakaguchi, Amuza Byaruhanga Lucky, Junya Yamagishi, Yuko Katakai, Satoru Kawai, Osamu Kaneko

In:

Scientific Reports (2022) :

DNA replication dynamics during erythrocytic schizogony in the malaria parasites Plasmodium falciparum and Plasmodium knowlesi

Categories:

Transfection

Authors:

Jennifer McDonald, Catherine J. Merrick

In:

PLoS Pathog (2022) :

Rapid and iterative genome editing in the malaria parasite Plasmodium knowlesi provides new tools for P. vivax research.

Categories:

Transfection

Authors:

Mohring F1, Hart MN1, Rawlinson TA2, Henrici R1, Charleston JA1, Diez Benavente E1, Patel A1, Hall J3, Almond N3, Campino S1, Clark TG1, Sutherland CJ1, Baker DA1, Draper SJ2, Moon RW1.

In:

eLife (2019) 17: 8

Regulation and essentiality of the START-domain-containing phospholipid transfer protein PFA0210c in malaria parasites.

Categories:

Transfection

Authors:

Hill RJ, Ringel A, Knuepfer E, Moon RW, Blackman MJ, van Ooij C.

In:

J Biol Chem (2016) 10(1): 1-30

Adaptation of the genetically tractable malaria pathogen Plasmodium knowlesi to continuous culture in human erythrocytes.

Categories:

Transfection

Authors:

Moon RW1, Hall J, Rangkuti F, Ho YS, Almond N, Mitchell GH, Pain A, Holder AA, Blackman MJ.

In:

Proc Natl Acad Sci USA (2013) 8: 531(6)