Neural progenitor (NHNP), human

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Neurospheres isolated from human brain cortex and cryopreserved.

Cell Type:
Neural SC
Tissue Origin:
brain
Species:
human
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Research Area:
Neurobiology
Stem Cells
Cell Characteristics:
Suspension

Recommended Media

Neural Progenitor Maintenance Medium

Neural Progenitor Maintenance Medium (NPMM) BulletKit contains the necessary supplements and media for optimal NHNP cell growth and differentiation.

This kit includes:
- CC-3210 – Neural Progenitor Basal Medium (200 ml)
- CC-4241 – Neural Progenitor Maintenance Medium SingleQuots Kit (contains hEGF and hFGF)
- CC-4242 – Neural Progenitor Supplement SingleQuots Kit (contains NSF-1 and GA)

Neural Progenitor Differentiation Medium

The Neural Progenitor Differentiation Medium (NPDM) BulletKit contains the necessary supplements and media for optimal NHNP differentiation. The medium can be customized by supplementation with differentiation-promoting agents such as brain-derived neurotrophic factor.

This kit includes:
- CC-3210 – Neural Progenitor Basal Medium
- CC-4242 – Neural Progenitor Supplement SingleQuots™ Kit

Transfection Information

In case no data are shown for the selected cell type, please take a look at our optimization strategy or contact our Scientific Support Teams to get further guidance on how to easily determine optimal Nucleofection conditions yourself.

Citations (26)

Microbiome-Derived Lipopolysaccharide (LPS) Selectively Inhibits neurofilament Light Chain (NF-L) Gene expression in Human Neuronal-Glial (HNG) Cells in Primary Culture
Categories:
Primary Cells and Media 
Authors:
Walker J. Lukiw, Lin Cong, Vivian Jaber, Yuhai Zhao 
In:
Frontiers in Molecular Neuroscience (2018) 12: 896 
Genomic DISC1 Disruption in hiPSCs Alters Wnt Signaling and Neural Cell Fate
Categories:
Transfection 
Authors:
Priya Srikanth, Karam Han, Dana G. Callahan, Eugenia Makovkina, Christina R. Muratore, Matthew A. Lalli, Honglin Zhou, Justin D. Boyd, Kenneth S. Kosik, Dennis J. Selkoe, Tracy L. Young-Pearse 
In:
Cell Rep (2015) 12: 1-16 
Genome-wide copy number profiling to detect gene amplifications in neural progenitor cells
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Fischera U, Kellerb A, Backesb C, Meesea E 
In:
Other (2014) ePub: ePub 
Differentiation of human pluripotent stem cells into nephron progenitor cells in a serum and feeder free system
Categories:
Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Kang M1, Han YM 
In:
PLoS ONE (2014) 9(4): e94888 
Three-dimensional normal human neural progenitor tissue-like assemblies: a model of persistent varicella-zoster virus infection
Categories:
Primary Cells and Media 
Authors:
Goodwin TJ, McCarthy M, Osterrieder N, Cohrs RJ, Kaufer BB. 
In:
PLoS Pathog (2013) 9(8): ePub 
microRNA (miRNA) speciation in Alzheimer's disease (AD) cerebrospinal fluid (CSF) and extracellular fluid (ECF).
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Alexandrov PN, Dua P, Hill JM, Bhattacharjee S, Zhao Y, Lukiw WJ. 
In:
Int J Biochem Mol Biol (2012) 3(4): 365-373 
A radial glia gene marker, fatty acid binding protein 7 (FABP7), is involved in proliferation and invasion of glioblastoma cells
Categories:
Primary Cells and Media, Transfection 
Authors:
De Rosa A, Pellegatta S, Rossi M, Tunici P, Magnoni L, Speranza MC, Malusa F, Miragliotta V, Mori E, Finocchiaro G, Bakker A. 
In:
PLoS ONE (2012) 7(12): ePub 
Snail depletes the tumorigenic potential of glioblastoma
Categories:
Primary Cells and Media 
Authors:
Savary K, Caglayan D, Caja L, Tzavlaki K, Bin Nayeem S, Bergström T, Jiang Y, Uhrbom L, Forsberg-Nilsson K, Westermark B, Heldin CH, Ferletta M, Moustakas 
In:
Oncogene (2012) 32(47): 5409-5420 
Metal-sulfate induced generation of ROS in human brain cells: detection using an isomeric mixture of 5- and 6-carboxy-2\\\',7\\\'-dichlorofluorescein diacetate (carboxy-DCFDA) as a cell permeant tracer.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Pogue AI, Jones BM, Bhattacharjee S, Percy ME, Zhao Y, Lukiw WJ 
In:
Int J Mol Sci (2012) 13(8): 9615-26 
Genome-wide gene amplification during differentiation of neural progenitor cells in vitro.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Fischer U, Keller A, Voss M, Backes C, Welter C, Meese E. 
In:
PLoS ONE (2012) 7(5): ePub 
Dickkopf 1 mediates glucocorticoid-induced changes in human neural progenitor cell proliferation and differentiation.
Categories:
Primary Cells and Media 
Authors:
Moors M, Bose R, Johansson-Haque K, Edoff K, Okret S, Ceccatelli S. 
In:
Toxicol Sci (2012) 125(2): 488-95 
EMMPRIN silencing inhibits proliferation and perineural invasion of human salivary adenoid cystic carcinoma cells in vitro and in vivo.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Yang X, Zhang P, Ma Q, Kong L, Li Y, Liu B, Lei D. 
In:
Cancer Biol Ther (2012) 13(2): 85-91 
Generation of Reactive Oxygen Species (ROS) and Pro-Inflammatory Signaling in Human Brain Cells in Primary Culture
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Lukiw WJ, Bjattacharjee S, Zhao Y, Pogue AI, Percy ME 
In:
Other (2012) Suppl: ePub 
Glioma-initiating cells retain their tumorigenicity through integration of the Sox axis and Oct4 protein
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Ikushima H, Todo T, Ino Y, Takahashi M, Saito N, Miyazawa K, Miyazono K. 
In:
J Biol Chem (2011) 286(48): 41434-41441 
Regulation of MET by FOXP2, genes implicated in higher cognitive dysfunction and autism risk
Categories:
 
Authors:
Mukamel Z, Konopka G, Wexler E, Osborn GE, Dong H, Bergman MY, Levitt P, Geschwind DH. 
In:
J Neurosci (2011) 31(32): 11437-11442 
Expression of GD2 and GD3 gangliosides in human embryonic neural stem cells
Categories:
Primary Cells and Media 
Authors:
Yanagisawa M, Yoshimura S, Yu RK 
In:
ACS Nano (2011) 3(2): e00054 
Human neurospheres as three-dimensional cellular systems for developmental neurotoxicity testing
Categories:
Primary Cells and Media 
Authors:
Moors M, Rockel TD, Abel J, Cline JE, Gassmann K, Schreiber T, Schuwald J, Weinmann N, Fritsche E. 
In:
Environ Health Perspect (2009) 117(7): 1131-8 
Targeting cancer stem cells through L1CAM suppresses glioma growth
Categories:
Primary Cells and Media 
Authors:
Bao S, Wu Q, Li Z, Sathornsumetee S, Wang H, McLendon RE, Hjelmeland AB, Rich JN. 
In:
Cancer Res (2008) 68(15): 6043-8 
Capsaicin-induced apoptosis of glioma cells is mediated by TRPV1 vanilloid receptor and requires p38 MAPK activation
Categories:
Primary Cells and Media 
Authors:
Amantini C, Mosca M, Nabissi M, Lucciarini R, Caprodossi S, Arcella A, Giangaspero F, Santoni G. 
In:
J Neurochem (2007) 102(3): 977-90 
CNS progenitor cells and oligodendrocytes are targets of chemotherapeutic agents in vitro and in vivo
Categories:
Primary Cells and Media 
Authors:
Dietrich J, Han R, Yang Y, Mayer-Pröschel M, Noble M. 
In:
J Cell Biol (2006) 5(7): 22 
Mesenchymal stem cells derived from CD133-positive cells in mobilized peripheral blood and cord blood: proliferation, Oct4 expression, and plasticity
Categories:
Primary Cells and Media 
Authors:
Tondreau T, Meuleman N, Delforge A, Dejeneffe M, Leroy R, Massy M, Mortier C, Bron D, Lagneaux L. 
In:
Stem Cells (2005) 23(8): 1105-12 
Polychlorinated biphenyls disturb differentiation of normal human neural progenitor cells: clue for involvement of thyroid hormone receptors
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Fritsche E, Cline JE, Nguyen NH, Scanlan TS, Abel J. 
In:
Environ Health Perspect (2005) 113(7): 871-6 
Absence of caspase 8 and high expression of PED protect primitive neural cells from cell death
Categories:
Primary Cells and Media 
Authors:
Ricci-Vitiani L, Pedini F, Mollinari C, Condorelli G, Bonci D, Bez A, Colombo A, Parati E, Peschle C, De Maria R. 
In:
J Exp Med (2004) 200(10): 1257-66 
HIV-1 promotes quiescence in human neural progenitor cells
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Krathwohl MD, Kaiser JL. 
In:
J Infect Dis (2004) 190(2): 216-26 
The neural progenitor-restricted isoform of the MARK4 gene in 19q13.2 is upregulated in human gliomas and overexpressed in a subset of glioblastoma cell lines.
Categories:
Primary Cells and Media 
Authors:
Beghini A, Magnani I, Roversi G, Piepoli T, Di Terlizzi S, Moroni RF, Pollo B, Fuhrman Conti AM, Cowell JK, Finocchiaro G, Larizza L. 
In:
Oncogene (2003) 22(17): 2581-91 
Immunocytochemical and physiological characterization of a population of cultured human neural precursors
Categories:
Primary Cells and Media 
Authors:
Piper DR, Mujtaba T, Rao MS, Lucero MT. 
In:
J Neurophysiol (2000) 84(1): 534-48 
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