Fibroblast, aortic adventitial, human

The outermost connective tissue covering the aorta.

Human aorta adventitial fibroblasts are isolated from tunica external of ascending or descending aorta.

Cell Type:
Fibroblast
Tissue Origin:
aortic
Species:
human
Research Area:
Cancer Research/Cell Biology
Cardiovascular
Cell Characteristics:
Adherent

Recommended Media

 Low serum growth medium developed to support the maintenance and expansion of prostate stromal cells.

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
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The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
Fibroblast, basic V-023 8e5 60-70% 70-75% Plasmid (general) 2 µg 100 µl I/II/2b

Citations (8)

Categories:
3D Cell Culture  
Authors:
Daniel Naveed Tavakol , Trevor R Nash , Youngbin Kim  Pamela L Graney , Martin Liberman , Sharon Fleischer , Roberta I Lock , Aaron O'Donnell , Leah Andrews , Derek Ning , Keith Yeager , Andrew Harken , Naresh Deoli , Sally A Amundson , Guy Garty , Kam W Leong , David J Brenner , Gordana Vunjak-Novakovic  
In:
(2024) :  
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Santiago JJ, McNaughton LJ, Koleini N1, Ma X, Bestvater B, Nickel BE, Fandrich RR, Wigle JT, Freed DH, Arora RC, Kardami E 
In:
PLoS ONE (2014) 9(5): e97281 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Blomkalns AL, Gavrila D, Thomas M, Neltner BS, Blanco VM, Benjamin SB, McCormick ML, Stoll LL, Denning GM, Collins SP, Qin Z, Daugherty A, Cassis LA, Thompson RW, Weiss RM, Lindower PD, Pinney SM, Chatterjee T, Weintraub NL. 
In:
J Am Heart Assoc (2013) 2(2): e000065 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Schnittker D1, Kwofie K, Ashkar A, Trigatti B, Richards CD. 
In:
Mediators Inflamm (2013) 2013: 317503 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Morimoto M, Yu Z, Stenzel P, Clewing JM, Najafian B, Mayfield C, Hendson G, Weinkauf JG, Gormley AK, Parham DM, Ponniah U, André JL, Asakura Y, Basiratnia M, Bogdanovic R, Bokenkamp A, Bonneau D, Buck A, Charrow J, Cochat P, Cordeiro I, Deschenes G, Fenkçi MS, Frange P, Fründ S, Fryssira H, Guillen-Navarro E, Keller K, Kirmani S, Kobelka C, Lamfers P, Levtchenko E, Lewis DB, Massella L, McLeod DR, Milford DV, Nobili F, Saraiva JM, Semerci CN, Shoemaker L, Stajic N, Stein A, Taha D, Wand D, Zonana J, Lücke T, Boerkoel CF. 
In:
Orphanet Journal of Rare Diseases (2012) 7: 70 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Robinson KG, Nie T, Baldwin AD, Yang EC, Kiick KL, Akins RE Jr. 
In:
J Biomed Mater Res A (2012) 100(5): 1356-67 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Tieu BC, Ju X, Lee C, Sun H, Lejeune W, Recinos A 3rd, Brasier AR, Tilton RG. 
In:
J Vasc Res (2011) 48(3): 261-72 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Nie T, Akins RE Jr, Kiick KL. 
In:
Acta Biomaterialia (2009) 5(3): 865-75 
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