cells

human neuroblastoma

Cell Type:

Neuroblast

Tissue Origin:

brain

Species:

human

Research Area:

Cancer Research/Cell Biology

Neurobiology

Cell Characteristics:

Adherent

Transfection Information

Lonza Optimized Protocol
Optimization Guideline

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The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.

Kit	Program	Cells	Efficiency	Viable Cells	Substrate		Format	Platform
SF	CA-137	2e5	81% ±2	80% ±7	Plasmid (general)	0.4 µg	20 µl	96-well
SF	CA-137	2e5	79-83%	73-87%	Plasmid (general)	0.4 µg	20 µl	4D X-Unit
V	G-004	1e6	72%		Plasmid (general)	2 µg	100 µl	I/II/2b
V	G-004	1e6	82%	40%	Plasmid (general)	2 µg	100 µl	I/II/2b
V	G-004	1e6	71%		Plasmid (general)	µg	100 µl	I/II/2b
V	A-023	1e6	42%		Plasmid (general)	µg	100 µl	I/II/2b
V	A-023	1e6	70%		Plasmid (general)	µg	100 µl	I/II/2b
V	A-023	1e6	63%		Plasmid (general)	µg	100 µl	I/II/2b

Citations (15)

Identification of Putative Target Genes of the Transcription Factor RUNX2

Categories:

Transfection

Authors:

M Kuhlwilm, A Davierwala, S Pääbo

In:

PLoS ONE (2013) 8 (12): e83218

Muscarinic receptor activation of AMP-activated protein kinase inhibits orexigenic neuropeptide mRNA expression

Categories:

Transfection

Authors:

Thornton C, Sardini A, Carling D

In:

J Biol Chem (2008) 283(25): 17116-22

Investigating the regulation of brain specific kinases 1 & 2 by phosphorylation

Categories:

Transfection

Authors:

Bright NJ, Carling D, Thornton C

In:

J Biol Chem (2008) 283(22): 14946-54

Targeting the Phosphoinositide 3-Kinase Isoform p110delta Impairs Growth and Survival in Neuroblastoma Cells

Categories:

Transfection

Authors:

Boller D, Schramm A, Doepfner KT, Shalaby T, von Bueren AO, Eggert A, Grotzer MA, Arcaro A

In:

Clin Cancer Res (2008) 14(4): 1172-1181

Parkin occurs in a stable, non-covalent, approximately 110-kDa complex in brain

Categories:

Transfection

Authors:

Van Humbeeck C, Waelkens E, Corti O, Brice A, Vandenberghe W

In:

Eur J Neurosci (2008) 27(2): 284-93

Activation of recombinant human TRPV1 receptors expressed in SH-SY5Y human neuroblastoma cells increases [Ca(2+)], initiates neurotransmitter release and promotes delayed cell death

Categories:

Transfection

Authors:

Lam PM, Hainsworth AH, Smith GD, Owen DE, Davies J, Lambert DG

In:

J Neurochem (2007) 102(3): 801-11

Phosphorylation of huntingtin by cyclin-dependent kinase 5 is induced by DNA damage and regulates wild-type and mutant huntingtin toxicity in neurons

Categories:

Transfection

Authors:

Anne SL, Saudou F, Humbert S

In:

J Neurosci (2007) 27(27): 7318-28

APLF (C2orf13) is a novel human protein involved in the cellular response to chromosomal DNA strand breaks

Categories:

Transfection

Authors:

Iles N, Rulten S, El-Khamisy SF, Caldecott KW

In:

Mol Cell Biol (2007) 27(10): 3793-803

Downregulation of Bim by brain-derived neurotrophic factor activation of TrkB protects neuroblastoma cells from paclitaxel but not etoposide or cisplatin-induced cell death

Categories:

Transfection

Authors:

Li Z, Zhang J, Liu Z, Woo CW, Thiele CJ

In:

Cell Death Differ (2007) 14(2): 318-326

TAp73 isoforms antagonize Notch signalling in SH-SY5Y neuroblastomas and in primary neurones

Categories:

Transfection

Authors:

Hooper C, Tavassoli M, Chapple JP, Uwanogho D, Goodyear R, Melino G, Lovestone S, Killick R

In:

J Neurochem (2006) 99(3): 989-99

LRRTM3 promotes processing of amyloid-precursor protein by BACE1 and is a positional candidate gene for late-onset Alzheimer's disease

Categories:

Transfection

Authors:

Majercak J, Ray WJ, Espeseth A, Simon A, Shi XP, Wolffe C, Getty K, Marine S, Stec E, Ferrer M, Strulovici B, Bartz S, Gates A, Xu M, Huang Q, Ma L, Shughrue P, Burchard J, Colussi D, Pietrak B, Kahana J, Beher D, Rosahl T, Shearman M, Hazuda D, Sachs AB, Koblan KS, Seabrook GR, Stone DJ

In:

Proc Natl Acad Sci USA (2006) 103(47): 17967-17972

A high-throughput drug screen targeted to the 5'untranslated region of Alzheimer amyloid precursor protein mRNA

Categories:

Transfection

Authors:

Bandyopadhyay S, Ni J, Ruggiero A, Walshe K, Rogers MS, Chattopadhyay N, Glicksman MA, Rogers JT

In:

J Biomol Screen (2006) 11(5): 469-80

BBC3 mediates fenretinide-induced cell death in neuroblastoma

Categories:

Transfection

Authors:

Wei JS, Whiteford CC, Cenacchi N, Son CG and Khan J

In:

Oncogene (2005) 24(54): 7976-7983

Bcl-2 regulator FKBP38 is activated by Ca(2+)/calmodulin

Categories:

Transfection

Authors:

Edlich F, Weiwad M, Erdmann F, Fanghanel J, Jarczowski F, Rahfeld JU und Fischer G

In:

EMBO J (2005) 24(14): 2688-2699

Neuropeptide FF (NPFF) analogs functionally antagonize opioid activities in NPFF2 receptor-transfected SH-SY5Y neuroblastoma cells

Categories:

Transfection

Authors:

Mollereau C, Mazarguil H, Zajac JM and Roumy M

In:

Mol Pharmacol (2005) 67(3): 965-975

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