SH-SY5Y

human neuroblastoma
Cell Type:
Neuroblast
Tissue Origin:
brain
Species:
human
Research Area:
Cancer Research/Cell Biology
Neurobiology
Cell Characteristics:
Adherent

Recommended Media

DMEM:F12 combines the richness of F12 with the higher component concentrations of DMEM. This medium is well suited for clonal density cultures and has been extensively used to demonstrate the effect of various hormones and growth factors on target tissues.

Storage = 2ºC to 8ºC

12-719 = 3.151 g/L glucose, L-glutamine and 15mM HEPES
BE04-687Q = 3.151 g/L glucose and L-glutamine, without HEPES
BE04-687F/U1 = 3.151 g/L glucose and Ultraglutamine I, without HEPES

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
SF 96-CA-137 2e5 81% ±2 80% ±7 Plasmid (general) 0.4 µg 20 µl Shuttle
SF

CA-137

2e5 79-83% 73-87% Plasmid (general) 0.4 µg 20 µl 4D X-Unit
V

G-004

1e6 72%


Plasmid (general) 2 µg 100 µl I/II/2b
V

G-004

1e6 82% 40% Plasmid (general) 2 µg 100 µl I/II/2b
V G-004 1e6 71% Plasmid (general) µg 100 µl I/II/2b
V A-023 1e6 42% Plasmid (general) µg 100 µl I/II/2b
V A-023 1e6 70% Plasmid (general) µg 100 µl I/II/2b
V A-023 1e6 63% Plasmid (general) µg 100 µl I/II/2b

Citations (15)

Categories:
Transfection 
Authors:
M Kuhlwilm, A Davierwala, S Pääbo 
In:
PLoS ONE (2013) 8 (12): e83218 
Categories:
Transfection 
Authors:
Thornton C, Sardini A, Carling D 
In:
J Biol Chem (2008) 283(25): 17116-22 
Categories:
Transfection 
Authors:
Bright NJ, Carling D, Thornton C 
In:
J Biol Chem (2008) 283(22): 14946-54 
Categories:
Transfection 
Authors:
Boller D, Schramm A, Doepfner KT, Shalaby T, von Bueren AO, Eggert A, Grotzer MA, Arcaro A 
In:
Clin Cancer Res (2008) 14(4): 1172-1181 
Categories:
Transfection 
Authors:
Van Humbeeck C, Waelkens E, Corti O, Brice A, Vandenberghe W 
In:
Eur J Neurosci (2008) 27(2): 284-93 
Categories:
Transfection 
Authors:
Lam PM, Hainsworth AH, Smith GD, Owen DE, Davies J, Lambert DG 
In:
J Neurochem (2007) 102(3): 801-11 
Categories:
Transfection 
Authors:
Anne SL, Saudou F, Humbert S 
In:
J Neurosci (2007) 27(27): 7318-28 
Categories:
Transfection 
Authors:
Iles N, Rulten S, El-Khamisy SF, Caldecott KW 
In:
Mol Cell Biol (2007) 27(10): 3793-803 
Categories:
Transfection 
Authors:
Li Z, Zhang J, Liu Z, Woo CW, Thiele CJ 
In:
Cell Death Differ (2007) 14(2): 318-326 
Categories:
Transfection 
Authors:
Hooper C, Tavassoli M, Chapple JP, Uwanogho D, Goodyear R, Melino G, Lovestone S, Killick R 
In:
J Neurochem (2006) 99(3): 989-99 
Categories:
Transfection 
Authors:
Majercak J, Ray WJ, Espeseth A, Simon A, Shi XP, Wolffe C, Getty K, Marine S, Stec E, Ferrer M, Strulovici B, Bartz S, Gates A, Xu M, Huang Q, Ma L, Shughrue P, Burchard J, Colussi D, Pietrak B, Kahana J, Beher D, Rosahl T, Shearman M, Hazuda D, Sachs AB, Koblan KS, Seabrook GR, Stone DJ 
In:
Proc Natl Acad Sci USA (2006) 103(47): 17967-17972 
Categories:
Transfection 
Authors:
Bandyopadhyay S, Ni J, Ruggiero A, Walshe K, Rogers MS, Chattopadhyay N, Glicksman MA, Rogers JT 
In:
J Biomol Screen (2006) 11(5): 469-80 
Categories:
Transfection 
Authors:
Wei JS, Whiteford CC, Cenacchi N, Son CG and Khan J 
In:
Oncogene (2005) 24(54): 7976-7983 
Categories:
Transfection 
Authors:
Edlich F, Weiwad M, Erdmann F, Fanghanel J, Jarczowski F, Rahfeld JU und Fischer G 
In:
EMBO J (2005) 24(14): 2688-2699 
Categories:
Transfection 
Authors:
Mollereau C, Mazarguil H, Zajac JM and Roumy M 
In:
Mol Pharmacol (2005) 67(3): 965-975 
+ Show All