INS1 832/13

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Insulin secreting cells (stably transfected with Plasmid coding human Proinsulin)

Cell Type:
Pancreas
Tissue Origin:
pancreas
Species:
rat
Research Area:
Gastroenterology
Cell Characteristics:
Suspension

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
V T-027 1e6 70% 85% Plasmid (general) 2 µg 100 µl I/II/2b
V T-020 1e6 55% 80% Plasmid (general) 2 µg 100 µl I/II/2b

Citations (12)

The MODY1 gene HNF4-alpha and a feedback loop control COUP-TFII expression in pancreatic beta cells
Categories:
Transfection 
Authors:
Perilhou A, Tourrel-Cuzin C, Zhang P, Kharroubi I, Wang H, Fauveau V, Scott DK, Wollheim CB, Vasseur-Cognet M 
In:
Mol Cell Biol (2008) 28(14): 4588-97 
Trefoil Factor 3 Stimulates Human and Rodent Pancreatic Islet beta-Cell Replication with Retention of Function
Categories:
Transfection 
Authors:
Fueger PT, Schisler JS, Lu D, Babu DA, Mirmira RG, Newgard CB, Hohmeier HE 
In:
Mol Endocrinol (2008) 22(5): 1251-9 
The Role of IRE1alpha in the Degradation of Insulin mRNA in Pancreatic beta-Cells
Categories:
Transfection 
Authors:
Lipson KL, Ghosh R, Urano F 
In:
PLoS ONE (2008) 3(2): e1648 
A role for ATP-citrate lyase, malic enzyme and pyruvate/citrate cycling in glucose-induced insulin secretion
Categories:
Transfection 
Authors:
Guay C, Madiraju SR, Aumais A, Joly E, Prentki M 
In:
J Biol Chem (2007) 282(49): 35657-65 
The mitochondrial citrate/isocitrate carrier plays a regulatory role in glucose-stimulated insulin secretion
Categories:
Transfection 
Authors:
Joseph JW, Jensen MV, Ilkayeva O, Palmieri F, Alarcon C, Rhodes CJ, Newgard CB 
In:
J Biol Chem (2006) 281(47): 35624-32 
A pyruvate cycling pathway involving cytosolic NADP-dependent isocitrate dehydrogenase regulates glucose-stimulated insulin secretion
Categories:
Transfection 
Authors:
Ronnebaum SM, Ilkayeva O, Burgess SC, Joseph JW, Lu D, Stevens RD, Becker TC, Sherry AD, Newgard CB, Jensen MV 
In:
J Biol Chem (2006) 281(41): 30593-30602 
Regulation of insulin biosynthesis in pancreatic beta cells by an endoplasmic reticulum-resident protein kinase IRE1
Categories:
Transfection 
Authors:
Lipson KL, Fonseca SG, Ishigaki S, Nguyen LX, Foss E, Bortell R, Rossini AA, Urano F 
In:
Cell Metab (2006) 4(3): 245-54 
Compensatory responses to pyruvate carboxylase suppression in islet beta -cells: preservation of glucose-stimulated insulin secretion
Categories:
Transfection 
Authors:
Jensen MV, Joseph JW, Ilkayeva O, Burgess S, Lu D, Ronnebaum SM, Odegaard M, Becker TC, Sherry AD, Newgard CB 
In:
J Biol Chem (2006) 281(31): 22342-22351 
Pro- and Antiapoptotic Proteins Regulate Apoptosis but Do Not Protect Against Cytokine-Mediated Cytotoxicity in Rat Islets and -Cell Lines
Categories:
Transfection 
Authors:
Collier JJ, Fueger PT, Hohmeier HE, Newgard CB 
In:
Diabetes (2006) 55(5): 1398-406 
A role for G(z) in pancreatic islet beta-cell biology
Categories:
Transfection 
Authors:
Kimple ME, Nixon AB, Kelly P, Bailey CL, Young KH, Fields TA and Casey PJ 
In:
J Biol Chem (2005) 280(36): 31708-31713 
WFS1 is a novel component of the unfolded protein response and maintains homeostasis of the endoplasmic reticulum in pancreatic beta-cells
Categories:
Transfection 
Authors:
Fonseca SG, Fukuma M, Lipson KL, Nguyen LX, Allen JR, Oka Y and Urano F 
In:
J Biol Chem (2005) 280(47): 39609-39615 
Involvement of a Mitochondrial Phosphatase in the Regulation of ATP Production and Insulin Secretion in Pancreatic beta Cells
Categories:
Transfection 
Authors:
Pagliarini DJ, Wiley SE, Kimple ME, Dixon JR, Kelly P, Worby CA, Casey PJ and Dixon JE 
In:
Mol Cell (2005) 19(2): 197-207 
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