BJAB

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Burkitt's lymphoma

Cell Type:
B cell
Tissue Origin:
blood
Species:
human
Research Area:
Cancer Research/Cell Biology
Immunotherapy / Hematology
Cell Characteristics:
Suspension

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
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The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
T T-016 5e6 78% 58% Plasmid (general) 2 µg 100 µl I/II/2b
T G-016 2e6 56% 91% Plasmid (general) 2 µg 100 µl I/II/2b
V C-009 2e6 47% 93% Plasmid (general) 2 µg 100 µl I/II/2b
V T-020 3e6 35-45% 70% Plasmid (general) 2 µg 100 µl I/II/2b
V C-009 3e6 30-40% 70% Plasmid (general) 2 µg 100 µl I/II/2b

Citations (11)

EBV noncoding RNA binds nascent RNA to drive host PAX5 to viral DNA
Categories:
Transfection 
Authors:
Lee N, Moss WN, Yario TA, Steitz JA 
In:
Cell (2015) 160(4): 607-18 
I?B kinase ß (IKBKB) mutations in lymphomas that constitutively activate canonical nuclear factor ?B (NF?B) signaling.
Categories:
Transfection 
Authors:
Kai X, Chellappa V, Donado C, Reyon D, Sekigami Y, Ataca D, Louissaint A, Mattoo H, Joung JK, Pillai S. 
In:
J Biol Chem (2014) 289(39): 26960-72 
In Vivo and in Vitro Intragenomic Rearrangement of TT Viruses
Categories:
Transfection 
Authors:
Leppik L, Gunst K, Lehtinen M, Dillner J, Streker K, de Villiers EM 
In:
J Virol (2007) 81(17): 9346-56 
Analysis of viral cis elements conferring Kaposi's sarcoma-associated herpesvirus episome partitioning and maintenance
Categories:
Transfection 
Authors:
Skalsky RL, Hu J, Renne R 
In:
J Virol (2007) 81(18): 9825-37 
Development of a fluorescence-based assay to screen antiviral drugs against Kaposi's sarcoma associated herpesvirus
Categories:
Transfection 
Authors:
Nun TK, Kroll DJ, Oberlies NH, Soejarto DD, Case RJ, Piskaut P, Matainaho T, Hilscher C, Wang L, Dittmer DP, Gao SJ, Damania B 
In:
Mol Cancer Ther (2007) 6(8): 2360-70 
Receptor-mediated endocytosis is not required for TRAIL-induced apoptosis
Categories:
Transfection 
Authors:
Kohlhaas SL, Craxton A, Sun XM, Pinkoski MJ, Cohen GM 
In:
J Biol Chem (2007) 282(17): 12831-41 
ZAP-70 enhances B-cell receptor signaling in spite of absent or inefficient tyrosine kinase activation in chronic lymphocytic leukemia and lymphoma B-cells
Categories:
Transfection 
Authors:
Gobessi S, Laurenti L, Longo PG, Sica S, Leone G, Efremov DG 
In:
Blood (2007) 109(5): 2032-9 
Specific resistance upon lentiviral TRAIL transfer by intracellular retention of TRAIL receptors
Categories:
Transfection 
Authors:
Wenger T, Mattern J, Penzel R, Gassler N, Haas TL, Sprick MR, Walczak H, Krammer PH, Debatin KM, Herr I 
In:
Cell Death Differ (2006) 13(10): 1740-51 
Intrabodies targeting the Kaposi's sarcoma-associated herpesvirus latency antigen inhibit viral persistence in lymphoma cells
Categories:
Transfection 
Authors:
Corte-Real S, Collins C, Aires da Silva F, Silmas P, Barbas Iii C, Chang Y, Moore P and Goncalves J 
In:
Blood (2005) 106(12): 3797-3802 
Global and Distinct Targets of IRF-5 and IRF-7 during Innate Response to Viral Infection
Categories:
Transfection 
Authors:
Barnes BJ, Richards J, Mancl M, Hanash S, Beretta L and Pitha PM 
In:
J Biol Chem (2004) 279(43): 45194-45207 
The SH3-SAM Adaptor HACS1 is Up-regulated in B Cell Activation Signaling Cascades
Categories:
Transfection 
Authors:
Zhu YX, Benn S, Li ZH, Wei E, Masih-Khan E, Trieu Y, Bali M, McGlade CJ, Claudio JO and Stewart AK 
In:
J Exp Med (2004) 200(6): 737-747 
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