For maximum re-differentiation and therefore maximum collagen production, Lonza’s Human Knee Articular Chondrocytes should be studied within the first passage after re-differentiation. Serial expansion in monolayer culture after re-differentiation will result in the cells losing their differentiated phenotype until the culture is almost 100% de-differentiated and no longer express collagen.

For optimal re-differentiation, cells should be expanded as few times as necessary prior to re-differentiation.