Chondrocyte, human (NHAC-kn)

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Articular cartilage cells, obtained post mortem.
Chondrocytes produce and maintain extracellular cartilage matrix. Cartilage provides joint cushioning and facilitates joint articulation.

Cell Type:
Cartilage
Tissue Origin:
cartilage
Species:
human
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Research Area:
Dermatology/Tissue Engineering
Cell Characteristics:
Adherent

Recommended Media

Chondrocyte Growth Media

CGM Bulletkit has been developed for the expansion of human chondrocytes.

Chondrocyte Differentiation Media

Media developed for the differentiation of chondrocytes

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
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The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
P3 96-ER-100 2e5 72-76% 72-96% Plasmid (general) 1 µg 20 µl 96-well
Chondrocyte, human U-024 5e5 55-75% 60-70% Plasmid (general) 5 µg 100 µl I/II/2b
P3

ER-100

 2e5 72-76% 72-96% Plasmid (general) 1 µg 20 µl 4D X-Unit

Citations (16)

Response of human chondrocytes and mesenchymal stromal cells to a decellularized human dermis
Categories:
Primary Cells and Media 
Authors:
Giavaresi G, Bondioli E, Melandri D, Giardino R, Tschon M, Torricelli P, Cenacchi G, Rotini R, Castagna A, Veronesi F, Pagani S, Fini M 
In:
BMC Musculoskelet Disord (2013) 14: 12 
A role for VEGFR2 activation in endothelial responses caused by barrier disruptive OxPAPC concentrations.
Categories:
Primary Cells and Media 
Authors:
Birukova AA, Lee S, Starosta V, Wu T, Ho T, Kim J, Berliner JA, Birukov KG. 
In:
PLoS ONE (2012) 1: e30957 
Rose hip and its constituent galactolipids confer cartilage protection by modulating cytokine, and chemokine expression.
Categories:
Primary Cells and Media 
Authors:
Schwager J, Hoeller U, Wolfram S, Richard N. 
In:
BMC Complemen Altern Med (2011) 11: 105 
A 3D cartilage - inflammatory cell culture system for the modeling of human osteoarthritis
Categories:
Primary Cells and Media 
Authors:
Sun L, Wang X, Kaplan DL. 
In:
Biomaterials (2011) 32(24): 5581-9 
Efficient non-viral transfection of primary human adult chondrocytes in a high-throughput format
Categories:
Transfection 
Authors:
Haag J, Voigt R, Soeder S, Aigner T 
In:
Osteoarthritis Cartilage (2008) epub: online 
Regulation of cartilage-specific gene expression in human chondrocytes by SirT1 and NAMPT
Categories:
Transfection 
Authors:
Dvir-Ginzberg M, Gagarina V, Lee EJ, Hall DJ 
In:
J Biol Chem (2008) epub: online 
Basic Fibroblast Growth Factor Activates the MAPK and NFB Pathways That Converge on Elk-1 to Control Production of Matrix Metalloproteinase-13 by Human Adult Articular Chondrocytes
Categories:
Transfection 
Authors:
Muddasani P, Norman JC, Ellman M, van Wijnen AJ, Im HJ 
In:
J Biol Chem (2007) 282(43): 31409-21 
Pro-inflammatory cytokine TNF-alpha induces BMP-2 in chondrocytes via mRNA stabilization and transcriptional up-regulation
Categories:
Transfection 
Authors:
Fukui N, Ikeda Y, Ohnuki T, Hikita A, Tanaka S, Yamane S, Suzuki R, Sandell LJ, Ochi T 
In:
J Biol Chem (2006) 281(37): 27229-41 
The Wnt-inducible transcription factor twist1 inhibits chondrogenesis
Categories:
Transfection 
Authors:
Reinhold MI, Kapadia RM, Liao Z and Naski MC 
In:
J Biol Chem (2006) 281(3): 1381-1388 
Inflammation-induced chondrocyte hypertrophy is driven by receptor for advanced glycation end products
Categories:
Transfection 
Authors:
Cecil DL, Johnson K, Rediske J, Lotz M, Schmidt AM and Terkeltaub R 
In:
J Immunol (2005) 175(12): 8296-8302 
Dehydroascorbate transport in human chondrocytes is regulated by hypoxia and is a physiologically relevant source of ascorbic acid in the joint
Categories:
Transfection 
Authors:
McNulty AL, Stabler TV, Vail TP, McDaniel GE, Kraus VB 
In:
Arthritis Rheum (2005) 52(9): 2676-85 
Articular chondrocytes express the receptor for advanced glycation end products: Potential role in osteoarthritis
Categories:
Transfection 
Authors:
Loeser RF, Yammani RR, Carlson CS, Chen H, Cole A, Im HJ, Bursch LS, Yan SD 
In:
Arthritis Rheum (2005) 52(8): 2376-85 
NF-kappaB mediates the stimulation of cytokine and chemokine expression by human articular chondrocytes in response to fibronectin fragments
Categories:
Transfection 
Authors:
Pulai JI, Chen H, Im HJ, Kumar S, Hanning C, Hegde PS and Loeser RF 
In:
J Immunol (2005) 174(9): 5781-5788 
TLR2 Signaling in Chondrocytes Drives Calcium Pyrophosphate Dihydrate and Monosodium Urate Crystal-Induced Nitric Oxide Generation
Categories:
Transfection 
Authors:
Liu-Bryan R, Pritzker K, Firestein GS and Terkeltaub R 
In:
J Immunol (2005) 174(8): 5016-5023 
New non-viral method for gene transfer into primary cells
Categories:
Transfection 
Authors:
Gresch O, Engel FB, Nesic D, Tran TT, England HM, Hickman ES, Korner I, Gan L, Chen S, Castro-Obregon S, Hammermann R, Wolf J, Muller-Hartmann H, Nix M, Siebenkotten G, Kraus G and Lun K 
In:
Methods (2004) 33(2): 151-163 
Efficient transfection method for primary cells
Categories:
Transfection 
Authors:
Hamm A, Krott N, Breibach I, Blindt R and Bosserhoff AK 
In:
Tissue Eng (2002) 8(2): 235-245 
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