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What is the intra-plate and inter-plate reproducibility of the RAFT™ System?

In terms of culture thickness, the intraplate variability is 10%; while the interplate variability is 8% (data obtained from measurements on 107 separate 96-well or 24-well insert plates).

How quickly can I produce a 3D cell culture using the RAFT™ System?

Using the RAFT™ System, it takes less than 1 hour to create 3D collagen cell cultures – individually or in batches depending on your research requirements.

How can I create co-cultures using the RAFT™3D Cell Culture System?

You can create co-cultures in different ways, which depend on the structure of the model you are trying to make. Below are three examples of co-cultures: 1. Two cell types mixed together inside one RAFT™ Culture: For this, it is possible to mix...

What are the advantages of the RAFT™ 3D Cell Culture System over coatings such as Matrigel or similar?

Growing cells in the RAFT™ 3D Culture System versus on any other coating including collagen, Matrigel™ or similar, comes to compare 3D to 2D cell culture. You might notice big differences in cells behavior and response. Cell-to-cell or -matrix...

What are the advantages of RAFT™ 3D Cultures over other 3D hydrogel cultures?

With the compression, the RAFT™ 3D Cell Culture System helsp to obtain high density collagens within a final concentration of 80 mg/ml that resemble to the in vivo tissue collagen density in the same magnitude. Classical hydrogels without...

Is the RAFT™ 3D Cell Culture permeable to large molecules like antibodies, drugs etc?

Although the RAFT™ Process concentrates the collagen matrix to a physiologically relevant concentration, the matrix itself does not act as a barrier to molecules as big as antibodies (150kDa), since no intensive permeabilisation steps were necessary...

Can you retrieve the cells from the RAFT™ Collagen Matrix by dissociation?

It is possible to dissociate the cells from the collagen matrix, by treating them with collagenase I or IV, both of which cleave the bond between a neutral amino acid (X) and glycine in the sequence Pro-X-Gly-Pro, which is found with high frequency...

Which inserts can be used with the RAFT™ Cell Culture Insert System?

To ensure optimum 3D cell culture production, it is recommended to use 6.5mm (in diameter) inserts from Greiner or Millipore, together with the relevant 24-well cell culture plates from these manufacturers - see below for full details. Inserts from...

How well do RAFT™ Cell Cultures mimic in vivo environment?

Several papers have been published describing the results of using this novel process and a range of methods and analytical approaches have been used which show that the cell cultures produced mimic the cells natural environment.

Can I change the concentration of collagen? How thick or thin can the RAFT™ System get the collagen layer?

The standard RAFT™ Protocol yields a culture of 100-120µm thickness at a collagen concentration of about 80mg/mL. It is possible to increase the collagen concentration to 100mg/mL by leaving the RAFT™ absorber in contact with the hydrogel for 30...