3D Cell Culture
Primary Cells and Media
Cancer Research/Cell Biology
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Is there a standard protocol available for the
™ 3D Cell Culture System?
Yes. A short protocol is included with
™ Kits. Detailed instructions can be accessed on www.lonza.com/
under product knowledge center tab.
Can I replace the
™ collagen with another hydrogel type ex. Matrigel?
Complete substitution for collagen type I within the
™ System (including its absorption step) is not possible, but one can certainly add Matrigel or laminins to the
™ Collagen Mixture, up to a certain ratio. The absorption conditions (ex...
How does the
™ System produce 3D cell cultures?
A mix of collagen, cells and other reagents is pipetted into the wells of a plate and incubated for 15 minutes to form a collagen hydrogel. Subsequently the biocompatible
™ Absorbers are placed on top of the gels and over a 15 minute period...
Which elements make up the
™ System – what do I buy?
™ System consists of the
™ Absorbers and a Reagent Kit. The elements each play their role in providing a robust, versatile solution to the creation of realistic, reproducible 3D cell cultures. For more information or to order
How can I image cells in a
™ 3D Cell Culture?
Below is a list of compatible imaging techniques and systems with
™ Cultures, depending on the type of cells being cultured.
™ Culture Types: Cells that tend to maintain a round morphology or that form higher order structures...
What cell seeding density should I use with the
Seeding density is cell and experiment dependent, however cell seeding density in 3D cell culture can be important for behavior and differentiation. The
™ 3D Cell Culture has a typical depth of 100-120µm. Therefore there is room for multiple...
Which cell types have already been grown using the
Since 2005, when Professor Robert Brown (Director, UCL Tissue Repair & Engineering Centre) developed the process, a wide variety of primary cells, stem cells and cell lines have been grown successfully using
™ System, for extended periods...
In which type of cell culture vessels are the
™ 3D Cell Cultures produced?
™ can produce the 3D cell cultures in each well of standard 96-well and 24-well cell culture plates as well as in 24-well cell culture inserts (6,5mm diameter, please contact Lonza Scientific Support for a list of
™ compatible cell culture...
What analytical techniques have been used with the
™3D Cell Culture System?
A broad range of techniques have been used with
™ Cultures. The list includes imaging, biochemical, and histological techniques. To view technical and application notes on analytical techniques visit www.lonza.com/
under product knowledge...
How would you further cultivate the cells after the formation of a
™ Cellular structure?
For the 96 and 24 well
™ Kit format, you simply put the media on top of the
structure laying already in the well, and cultivate the cells, as you classically do in 2 dimensions. For high cell number in
™ Cultures you might use twice...
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