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Is there a standard protocol available for the RAFT™ 3D Cell Culture System?

Yes. A short protocol is included with RAFT™ Kits. Detailed instructions can be accessed on www.lonza.com/raft under product knowledge center tab.

Can I replace the RAFT™ collagen with another hydrogel type ex. Matrigel?

Complete substitution for collagen type I within the RAFT™ System (including its absorption step) is not possible, but one can certainly add Matrigel or laminins to the RAFT™ Collagen Mixture, up to a certain ratio. The absorption conditions (ex...

How does the RAFT™ System produce 3D cell cultures?

A mix of collagen, cells and other reagents is pipetted into the wells of a plate and incubated for 15 minutes to form a collagen hydrogel. Subsequently the biocompatible RAFT™ Absorbers are placed on top of the gels and over a 15 minute period...

Which elements make up the RAFT™ System – what do I buy?

The RAFT™ System consists of the RAFT™ Absorbers and a Reagent Kit. The elements each play their role in providing a robust, versatile solution to the creation of realistic, reproducible 3D cell cultures. For more information or to order RAFT...

How can I image cells in a RAFT™ 3D Cell Culture?

Below is a list of compatible imaging techniques and systems with RAFT™ Cultures, depending on the type of cells being cultured. RAFT™ Culture Types: Cells that tend to maintain a round morphology or that form higher order structures...

What cell seeding density should I use with the RAFT™ System?

Seeding density is cell and experiment dependent, however cell seeding density in 3D cell culture can be important for behavior and differentiation. The RAFT™ 3D Cell Culture has a typical depth of 100-120µm. Therefore there is room for multiple...

Which cell types have already been grown using the RAFT™ Process?

Since 2005, when Professor Robert Brown (Director, UCL Tissue Repair & Engineering Centre) developed the process, a wide variety of primary cells, stem cells and cell lines have been grown successfully using RAFT™ System, for extended periods...

In which type of cell culture vessels are the RAFT™ 3D Cell Cultures produced?

RAFT™ can produce the 3D cell cultures in each well of standard 96-well and 24-well cell culture plates as well as in 24-well cell culture inserts (6,5mm diameter, please contact Lonza Scientific Support for a list of RAFT™ compatible cell culture...

What analytical techniques have been used with the RAFT™3D Cell Culture System?

A broad range of techniques have been used with RAFT™ Cultures. The list includes imaging, biochemical, and histological techniques. To view technical and application notes on analytical techniques visit www.lonza.com/raft under product knowledge...

How would you further cultivate the cells after the formation of a RAFT™ Cellular structure?

For the 96 and 24 well RAFT™ Kit format, you simply put the media on top of the RAFT structure laying already in the well, and cultivate the cells, as you classically do in 2 dimensions. For high cell number in RAFT™ Cultures you might use twice...
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