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Is there a special reagent kit for the RAFT™ System?

Yes. The RAFT™ Reagent Kit contains high quality controlled acidified rat tail type I collagen, a proprietary neutralizing solution for ease of use and reproducibility and 10 x MEM solution for enrichment of the collagen solution. The kit contains...

What is special about the RAFT™ Process?

The RAFT™ Process allows the user to control the process of making 3D cell cultures. The RAFT™ System enables the researcher to determine the cell seeding density in their cultures and supports more complex cell co-culture. Cells can be cultured...

What is the RAFT™ 3D Cell Culture System?

RAFT™ System is a superior 3D cell culture system to enable the production of collagen-based cultures, simply and reliably. It combines a novel patented process with specially-designed RAFT™ Absorbers and reagents in combination with standard cell...

Why is collagen used in the formation of RAFT™ Cultures?

The RAFT™ System uses type I collagen, the major component of natural extracellular matrix (ECM), as it is the ideal material for forming realistic 3D cell cultures. The patented RAFT™ Process enables a physiologically relevant concentration...

Can I use antibiotics and antimicotics with RAFT™ Cultures?

Yes. Antibiotics and antimicotics can be added to the RAFT™ Cell Culture Medium, if desired. Usually it should be fine to follow your established cell culture protocols or the standard recommendations for antibiotics/antimicotics given by your cell...

Can I modify the RAFT™ Standard Protocol?

Yes. The standard protocol is an excellent starting point for using the RAFT™ System however the system allows the researcher to modify the conditions to better suit their cells and experimental design. For instance the volume of collagen...

Can I replace the RAFT™ collagen with another hydrogel type ex. Matrigel?

Complete substitution for collagen type I within the RAFT™ System (including its absorption step) is not possible, but one can certainly add Matrigel or laminins to the RAFT™ Collagen Mixture, up to a certain ratio. The absorption conditions (ex...

How can I image cells in a RAFT™ 3D Cell Culture?

Below is a list of compatible imaging techniques and systems with RAFT™ Cultures, depending on the type of cells being cultured. RAFT™ Culture Types: Cells that tend to maintain a round morphology or that form higher order structures...

Which elements make up the RAFT™ System – what do I buy?

The RAFT™ System consists of the RAFT™ Absorbers and a Reagent Kit. The elements each play their role in providing a robust, versatile solution to the creation of realistic, reproducible 3D cell cultures. For more information or to order RAFT...

What is the RAFT™ 24-well Insert Absorber Kit made for?

The RAFT™ 24-well Insert Absorbers work with 6.5mm (in diameter) cell culture inserts. Therefore they can be used to make RAFT™ cultures in cell culture inserts and are designed for applications like air-lift skin models or blood-brain barrier...
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