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Cell Information
(8)
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(120)
FAQ
(4)
Culture Media
(5)
Category
Transfection
(3)
Serum-free and Speciality Media
(2)
Classical Media and Reagents
(1)
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Basic Research
(2)
Gene Expression
(2)
Cancer Research/Cell Biology
(1)
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My suspension-adapted HEK293 (or
CHO
) cells tend to clump in culture. How can I avoid this?
Efficient protein production experiments require growth of the host cell in single-cell suspension, something that is sometimes difficult to achieve since most established cell lines retain their adherent growth characteristics. Commercially...
Do your Pro
CHO
™ Media contain hypoxanthine and thymidine?
All of our Pro
CHO
™ Media, doe not contain Thymidine and Hypoxanthine. So if the customer has a
CHO
Clone which is dhfr-negativ, he will need to add Thymidine and Hypoxanthine or otherwise the cells will not grow. If the customer has a
CHO
clone...
Why is the Nucleofector® Technology ideal for transient protein production ?
For many cell lines in suspension, especially s
CHO
, conventional transfection methods are very limited in efficiency. For protein production, suspension cells are favored over adherent cells because they are much easier to cultivate. Unlike...
Which promoter can be recommended for transient gene expression in mammalian cells ?
Vectors with a CMV promoter typically work fine for transient protein expression in mammalian cells, like HEK293,
CHO
or NSO. For insect cells, like Sf9 or Sf21, vectors with insect promoters are required. In HEK293E (EBNA) cells, the use...