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Co-culture protocol for Keratinocytes and
Fibroblast
s (2D)
If you are searching for a 2D co-culture protocol for matched donor Keratinocytes and
Fibroblasts
, please find our co-culture protocol on Matched Donor Keratinocytes and
Fibroblasts
2D Co-Culture ProtocolFurthermore, here is our White Paper...
Where are Lonza’s dermal
fibroblast
s (NHDF) isolated from?
Lonza’s offers dermal
fibroblasts
from both adult and neonatal donors. Lonza’s adult dermal
fibroblasts
(NHDF-Ad) are usually isolated from tissue obtained from elective cosmetic surgical procedures. Generally, these procedures are abdominoplasties...
Where are Lonza’s aortic adventitial
fibroblast
s (AoAF) isolated from?
Lonza’s aortic adventitial
fibroblasts
(AoAF) are isolated from the outer connective tissue layer of the aorta. The aortia is divided into three layers. The tunica intima is the inner endothelial layer, the tunica media is the layers of the smooth...
Where are Lonza’s lung
fibroblast
s (NHLF and DHLF) isolated from?
Lonza’s lung
fibroblasts
are not taken from any specific area of the lung but instead are taken from an area that appears to be in good shape and is non-hemorrhagic. The pleural membrane is removed and the peripheral tissue is used. Enzymatic...
Where are Lonza’s Ventricular Normal Human Cardiac
Fibroblast
cells isolated from?
Lonza obtains our Ventricular Normal Human Cardiac
Fibroblast
(NHCF-V) from left ventricle.
Where are Lonza’s Atrial Normal Human Cardiac
Fibroblast
cells isolated from?
Lonza obtains our Atrial Normal Human Cardiac
Fibroblast
(NHCF-A) from right atrium.
Can an alternate to the 5-bromo-2'-deoxyuridine (BrdU) be used to control the growth of
fibroblast
s when culturing rat cardiac myocytes?
At this time, we can only recommend the use of BrdU for controlling the growth of
fibroblasts
.When developing this product, we tried using cytosine beta-arabino furanoside (CAF) to inhibit
fibroblast
growth. While CAF successfully inhibited...
What is the difference between the FBM™
Fibroblast
Growth Basal Medium CC-3131 and the SCBM™ Stromal Cell Growth Basal Medium CC-3204? Can the FBM™ Basal Medium and the SCBM™ Basal Medium be used interchangeable? Is there a phenol red free version of the FBM™ Basal Medium?
The FBM™ Basal Medium and the SCBM™ Basal Medium are essentially the same except that the FBM™ Basal Medium contains phenol red and the SCBM™ Basal Medium does not. The FBM™ Basal Medium and the SCBM™ Basal Medium can always be used interchangeably...
Can hydrocortisone be excluded from the Clonetics® BulletKit® when culturing cells? What is the purpose of hydrocortisone in the culture media?
Hydrocortisone is used for at least two purposes in Lonza’s primary cell culture media. Hydrocortisone (in combination with low serum concentrations) will inhibit the takeover of the culture by
fibroblasts
which may be present in small numbers from...
What is the purpose of the 5-bromo-2'-deoxyuridine (BrdU) when culturing rat cardiac myocytes?
5-bromo-2'-deoxyuridine (BrdU) is very efficient at inhibiting the proliferation of
fibroblasts
which are natively present at the time of isolation. Without the addition of the BrdU, the
fibroblasts
would overtake the culture.
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