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Transient genetic labeling of human CD34-positive hematopoietic stem cells using nucleofection

Background: Human hematopoietic stem cells (HSCs) are used for autologous and allogeneic stem cell transplantation. Currently, HSCs serve as target cells for tissue engineering, gene transfer, and gene therapeutic approaches. However, viral...

Authors: Greiner J, Wiehe J, Wiesneth M, Zwaka TP, Prill T, Schwarz K, Bienek-Ziolkowski M, Schmitt M, Döhner...

SST Peer Reviewed

IkappaB kinase promotes tumorigenesis through inhibition of forkhead FOXO3a

Nuclear exclusion of the forkhead transcription factor FOXO3a by protein kinase Akt contributes to cell survival. We investigated the pathological relationship between phosphoylated-Akt (Akt-p) and FOXO3a in primary tumors. Surprisingly, FOXO3a was...

Authors: Hu MC, Lee DF, Xia W, Golfman LS, Ou-Yang F, Yang JY, Zou Y, Bao S, Hanada N, Saso H, Kobayashi R...

Dynamic molecular interactions linking the T cell antigen receptor to the actin cytoskeleton

T cell receptor (TCR) engagement leads to actin polymerization at the site of T cell contact with antigen-presenting cells. Here we have studied the dynamic activity of proteins involved in regulating actin polymerization in live T cells after...

Authors: Barda-Saad M, Braiman A, Titerence R, Bunnell SC, Barr VA and Samelson LE

Association of ABCA1 with Syntaxin 13 and Flotillin-1 and Enhanced Phagocytosis in Tangier Cells

The ATP-binding cassette transporter A1 (ABCA1) facilitates the cellular release of cholesterol and choline-phospholipids to apolipoprotein A-I (apoA-I) and several studies indicate that vesicular transport is associated with ABCA1 function....

Authors: Bared SM, Buechler C, Boettcher A, Dayoub R, Sigruener A, Grandl M, Rudolph C, Dada A and Schmitz G

New invMED1 element cis-activates human multidrug-related MDR1 and MVP genes, involving the LRP130 protein

The MDR1 gene is a key component of the cytotoxic defense network and its overexpression results in the multidrug resistance (MDR) phenotype. However, the molecular mechanisms that regulate the MDR1 gene and coordinate multiple MDR-related genes...

Authors: Labialle S, Dayan G, Gayet L, Rigal D, Gambrelle J and Baggetto LG

Reduction of cysteine sulfinic acid by sulfiredoxin is specific to 2-Cys peroxiredoxins

Cysteine residues of certain peroxiredoxins (Prxs) undergo reversible oxidation to sulfinic acid (Cys-SO(2)H) and the reduction reaction is catalyzed by sulfiredoxin (Srx). Specific Cys residues of various other proteins are also oxidized to sulfinic...

Authors: Woo HA, Jeong W, Chang TS, Park KJ, Park SJ, Yang JS and Rhee SG

Distinct cAMP signaling pathways differentially regulate 2C-adrenoceptor expression: role in serum induction in human arteriolar smooth muscle cells

The physiological role of alpha(2)-adrenoceptors (alpha(2)-ARs) in cutaneous, arteriolar, vascular smooth muscle cells (VSMs) is to mediate cold-induced constriction. In VSMs cultured from human cutaneous arterioles, there is a selective increase in...

Authors: Chotani MA, Mitra S, Eid AH, Han SA and Flavahan NA

Efficient transfection of embryonic and adult stem cells

The ability of embryonic stem cells and adult stem cells to differentiate into specific cell types holds immense potential for therapeutic use in cell and gene therapy. Realization of this potential depends on efficient and optimized protocols for...

Authors: Lakshmipathy U, Pelacho B, Sudo K, Linehan JL, Coucouvanis E, Kaufman DS and Verfaillie CM

SST Peer Reviewed

T-cell Activation Leads to Poor Activation of the HIV-1 Clade E Long Terminal Repeat and Weak Association of Nuclear Factor-B and NFAT with Its Enhancer Region

The enhancer region in the human immunodeficiency virus type 1 (HIV-1) 5'-long terminal repeat (LTR) is very important for viral transcription. This promoter sequence binds both nuclear factor-kappaB and NFAT, two important modulators of HIV-1 gene...

Authors: Lemieux AM, Pare ME, Audet B, Legault E, Lefort S, Boucher N, Landry S, van Opijnen T, Berkhout B,...

mRNA but not plasmid DNA is efficiently transfected in murine J774A.1 macrophages

Previous studies demonstrated that macrophages are difficult to transfect. In the present study, we investigated whether J774A.1 macrophages can be efficiently transfected using nucleofector technology. Nucleofection of J774A.1 macrophages with mRNA...

Authors: Van De Parre TJ, Martinet W, Schrijvers DM, Herman AG and De Meyer GR

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