Search Results

MicroRNA-320 family is downregulated in colorectal adenoma and affects tumor proliferation by targeting CDK6

To investigate the microRNA (miRNA) expression during histological progression from colorectal normal mucosa through adenoma to carcinoma within a lesion. Using microarray, the sequential changes in miRNA expression profiles were compared in colonic...

Authors: Toshihiro Tadano, Yoichi Kakuta, Shin Hamada, Yosuke Shimodaira, Masatake Kuroha, Yoko Kawakami,...

Targeting the metabolic pathway of human colon cancer overcomes resistance to TRAIL-induced apoptosis.

Colon cancer is a leading cause of cancer-related mortality for which targeted therapy is needed; however, trials using apoptosis-inducing ligand monotherapy to overcome resistance to apoptosis have not shown clinical responses. Since colon cancer...

Authors: Carr RM, Qiao G, Qin J, Jayaraman S, Prabhakar BS, Maker AV

The transcription factor ZEB1 promotes an aggressive phenotype in prostate cancer cell lines

It has been reported that one of the factors that promotes tumoral progression is the abnormal activation of the epithelial-mesenchymal transition program. This process is associated with tumoral cells acquiring invasive and malignant properties and...

Authors: Orellana-Serradell O1, Herrera D1, Castellon EA1, Contreras HR1.

Generation of inner ear organoids containing functional hair cells from human pluripotent stem cells.

The derivation of human inner ear tissue from pluripotent stem cells would enable in vitro screening of drug candidates for the treatment of hearing and balance dysfunction and may provide a source of cells for cell-based therapies of the inner ear....

Authors: Koehler KR, Nie J1, Longworth-Mills E, Liu XP, Lee J, Holt JR, Hashino E.

SST Peer Reviewed

Dose intensification of TRAIL-inducing ONC201 inhibits metastasis and promotes intratumoral NK cell recruitment.

ONC201 is a first-in-class, orally active antitumor agent that upregulates cytotoxic TRAIL pathway signaling in cancer cells. ONC201 has demonstrated safety and preliminary efficacy in a first-in-human trial in which patients were dosed every 3...

Authors: Wagner J1, Kline CL1, Zhou L1, Campbell KS2, MacFarlane AW2, Olszanski AJ3, Cai KQ4, Hensley HH5,...

Generation of a human induced pluripotent stem cell line via CRISPR-Cas9 mediated integration of a site-specific homozygous mutation in CHMP2B.

Frontotemporal dementia (FTD) is an early onset neurodegenerative disease. Mutations in several genes cause familial FTD and one of them is charged multivesicular body protein 2B (CHMP2B) on chromosome 3 (FTD3), a component of the endosomal sorting...

Authors: Zhang Y, Schmid B, Nielsen TT, Nielsen JE, Clausen C, Hyttel P, Holst B, Freude KK.

SST Peer Reviewed

Quadrupling efficiency in production of genetically modified pigs through improved oocyte maturation.

Assisted reproductive technologies in all mammals are critically dependent on the quality of the oocytes used to produce embryos. For reasons not fully clear, oocytes matured in vitro tend to be much less competent to become fertilized, advance to...

Authors: Yuan Y1,2, Spate LD3, Redel BK3, Tian Y3,2, Zhou J4, Prather RS3, Roberts RM1,2

DNA methylation is extensively remodeled during mammalian gametogenesis and embryogenesis. Most transposons become hypomethylated, raising the question of their regulation in the absence of DNA methylation. To reproduce a rapid and extensive demethylation, we subjected mouse ES cells to chemically defined hypomethylating culture conditions. Surprisingly, we observed two phases of transposon regulation. After an initial burst of de-repression, various transposon families were efficiently re-silenced. This wa

DNA methylation is extensively remodeled during mammalian gametogenesis and embryogenesis. Most transposons become hypomethylated, raising the question of their regulation in the absence of DNA methylation. To reproduce a rapid and extensive...

Authors: Walter M, Teissandier A, Pérez-Palacios R, Bourc\\\'his D.

Fiber-based fluorescence lifetime imaging of recellularization processes on vascular tissue constructs

New techniques able to monitor the maturation of tissue engineered constructs over time are needed for a more efficient control of developmental parameters. Here, a label-free fluorescence lifetime imaging (FLIm) approach implemented through a single...

Authors: Alfonso-Garcia A1, Shklover J1, Sherlock BE1, Panitch A1, Griffiths LG2, Marcu L1

Highly efficient Cas9-mediated transcriptional programming.

The RNA-guided nuclease Cas9 can be reengineered as a programmable transcription factor. However, modest levels of gene activation have limited potential applications. We describe an improved transcriptional regulator obtained through the rational...

Authors: Chavez A1, Scheiman J2, Vora S3, Pruitt BW4, Tuttle M4, P R Iyer E2, Lin S5, Kiani S6, Guzman CD4,...

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