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Differential cellular responses to exogenous DNA in mammalian cells and its effect on oligonucleotide-directed gene modification

Transient transfection has been widely used in many biological applications including gene regulation and DNA repair, but, so far, little attention has been paid to cellular responses induced by the transfected DNA. Here, we report that...

Authors: Igoucheva O, Alexeev V and Yoon K

G-CSF induced reactive oxygen species involves Lyn-PI 3-kinase-Akt and contributes to myeloid cell growth

Granulocyte colony-stimulating factor (G-CSF) is a pleiotropic cytokine that drives the production, survival, differentiation, and inflammatory functions of granulocytes. Reactive oxygen species (ROS) provide a major thrust of the inflammatory...

Authors: Zhu QS, Xia L, Mills GB, Lowell CA, Touw IP and Corey SJ

Gephyrin regulates the cell surface dynamics of synaptic GABAA receptors

The efficacy of fast synaptic inhibition is critically dependent on the accumulation of GABAA receptors at inhibitory synapses, a process that remains poorly understood. Here, we examined the dynamics of cell surface GABAA receptors using receptor...

Authors: Jacob TC, Bogdanov YD, Magnus C, Saliba RS, Kittler JT, Haydon PG and Moss SJ

Probucol enhances the expression of human hepatic scavenger receptor class B type I, possibly through a species-specific mechanism

OBJECTIVE: Scavenger receptor class B type I (SR-BI) is a major receptor for high-density lipoproteins (HDL) in the liver, which is the terminus of reverse cholesterol transport. Overexpression of SR-BI attenuated experimental atherosclerosis in...

Authors: Hirano KI, Ikegami C, Tsujii KI, Zhang Z, Matsuura F, Nakagawa-Toyama Y, Koseki M, Masuda D,...

Epstein-Barr virus lytic infection contributes to lymphoproliferative disease in a SCID mouse model

Most Epstein-Barr virus (EBV)-positive tumor cells contain one of the latent forms of viral infection. The role of lytic viral gene expression in EBV-associated malignancies is unknown. Here we show that EBV mutants that cannot undergo lytic viral...

Authors: Hong GK, Gulley ML, Feng WH, Delecluse HJ, Holley-Guthrie E and Kenney SC

Transforming Growth Factor 1 Induces Nox 4 NAD(P)H Oxidase and Reactive Oxygen Species-Dependent Proliferation in Human Pulmonary Artery Smooth Muscle Cells

Transforming growth factor beta1 (TGFbeta1) is abundantly expressed in pulmonary hypertension, but its effect on the pulmonary circulation remain unsettled. We therefore studied TGFbeta1 stimulation of freshly isolated human pulmonary artery smooth...

Authors: Sturrock A, Cahill B, Norman K, Huecksteadt TP, Hill K, Sanders K, Karwande SV, Stringham JC, Bull...

Towards systematic identification of Plasmodium essential genes by transposon shuttle mutagenesis

After the deciphering of the genome sequences of several Plasmodium species, efforts must turn to elucidating gene function and identifying essential gene products. However, random approaches are lacking and gene targeting is inefficient in...

Authors: Sakamoto H, Thiberge S, Akerman S, Janse CJ, Carvalho TG and Menard R

The human CD94 gene encodes multiple, expressible transcripts including a new partner of NKG2A/B

CD94/NKG2A is an inhibitory receptor expressed by natural killer (NK) cells and a subset of CD8+ T cells. Ligation of CD94/NKG2A by its ligand HLA-E results in tyrosine phosphorylation of the NKG2A immunoreceptor tyrosine-based inhibitory motifs, and...

Authors: Lieto LD, Maasho K, West D, Borrego F and Coligan JE

Immediate transfection of patient-derived leukemia: a novel source for generating cell-based vaccines

BACKGROUND: The production of cell-based cancer vaccines by gene vectors encoding proteins that stimulate the immune system has advanced rapidly in model systems. We sought to develop non-viral transfection methods that could transform patient tumor...

Authors: Gershan JA, Johnson BD, Weber J, Schauer DW, Natalia N, Behnke S, Burns K, Maloney KW, Warwick AB...

Autoimmune-associated lymphoid tyrosine phosphatase is a gain-of-function variant

A SNP in the gene PTPN22 is associated with type 1 diabetes, rheumatoid arthritis, lupus, Graves thyroiditis, Addison disease and other autoimmune disorders. T cells from carriers of the predisposing allele produce less interleukin-2 upon TCR...

Authors: Vang T, Congia M, Macis MD, Musumeci L, Orru V, Zavattari P, Nika K, Tautz L, Tasken K, Cucca F,...

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