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Live-cell imaging reveals sequential oligomerization and local plasma membrane targeting of stromal interaction molecule 1 after Ca2+ store depletion

Stromal interaction molecule 1 (STIM1) has recently been identified by our group and others as an endoplasmic reticulum (ER) Ca(2+) sensor that responds to ER Ca(2+) store depletion and activates Ca(2+) channels in the plasma membrane (PM). The...

Authors: Liou J, Fivaz M, Inoue T, Meyer T

Interaction of PRP4 with Kruppel-Like Factor 13 Regulates CCL5 Transcription

Activation of resting T lymphocytes initiates differentiation into mature effector cells over 3-7 days. The chemokine CCL5 (RANTES) and its major transcriptional regulator, Krüppel-like factor 13 (KLF13), are expressed late (3-5 days) after...

Authors: Huang B, Ahn YT, McPherson L, Clayberger C, Krensky AM

Expression and translocation of fluorescent-tagged p21-activated kinase-binding domain and PH domain of protein kinase B during murine neutrophil chemotaxis

Neutrophils are key cells of the innate immune system; they are terminally differentiated and therefore difficult to genetically manipulate and study in vitro. In the present study, we describe a protocol to transiently express two fluorescent...

Authors: Magalhaes MA, Zhu F, Sarantis H, Gray-Owen SD, Ellen RP, Glogauer M

Low dose arsenic trioxide sensitizes glucocorticoid-resistant acute lymphoblastic leukemia cells to dexamethasone via an Akt-dependent pathway

Incorporation of apoptosis-inducing agents into current therapeutic regimens is an attractive strategy to improve treatment for drug resistant leukemia. We tested the potential of arsenic trioxide (ATO) to restore the response to dexamethasone in...

Authors: Bornhauser BC, Bonapace L, Lindholm D, Martinez R, Cario G, Schrappe M, Niggli FK, Schafer BW,...

Nodes of Ranvier and axon initial segments are ankyrin G-dependent domains that assemble by distinct mechanisms

Axon initial segments (AISs) and nodes of Ranvier are sites of action potential generation and propagation, respectively. Both domains are enriched in sodium channels complexed with adhesion molecules (neurofascin [NF] 186 and NrCAM) and cytoskeletal...

Authors: Dzhashiashvili Y, Zhang Y, Galinska J, Lam I, Grumet M, Salzer JL

Modulation of the Cellular Accumulation and Intracellular Activity of Daptomycin towards phagocytized Staphylococcus aureus by the P-glycoprotein (MDR1) Efflux Transporter in human THP-1 macrophages and Madin-Darby canine kidney cells

We have examined its effect on the modulation of the intracellular accumulation and activity of daptomycin towards phagocytized S. aureus (ATCC 25923) in human THP-1 macrophages, in comparison with MDCK epithelial cells (wild type and MDCK-MDR1...

Authors: Lemaire S, Van Bambeke F, Mingeot-Leclercq MP, Tulkens PM

Rate, extent and concentration-dependence of histamine-evoked Weibel-Palade body exocytosis determined from individual fusion events in human endothelial cells

The rate, concentration dependence and extent of histamine-evoked Weibel-Palade body (WPB) exocytosis were investigated with time-resolved fluorescence microscopy in cultured human umbilical vein endothelial cells expressing WPB-targeted chimeras of...

Authors: Erent M, Meli A, Moisoi N, Babich V, Hannah MJ, Skehel P, Knipe L, Zupancic G, Ogden D, Carter TD

Nerve growth factor stimulates the concentration of TrkA within lipid rafts and extracellular signal-regulated kinase activation through c-Cbl-associated protein

Nerve growth factor (NGF) acts through its receptor, TrkA, to elicit the neuronal differentiation of PC12 cells through the action of extracellular signal-regulated kinase 1 (ERK1) and ERK2. Upon NGF binding, TrkA translocates and concentrates in...

Authors: Limpert AS, Karlo JC, Landreth GE

MEK1/2 inhibitors potentiate UCN-01 lethality in human multiple myeloma cells through a Bim-dependent mechanism

The role of Bim in synergistic interactions between UCN-01 and MEK1/2 inhibitors in human multiple myeloma cells was investigated. Exposure of U266 or RPMI8226 cells to UCN-01 resulted in ERK1/2 activation-associated BimEL...

Authors: Pei XY, Dai Y, Tenorio S, Lu J, Harada H, Dent P, Grant S

The Zinc Finger Protein RREB-1 is a Co-Regulator of the Androgen Receptor: Implications for the Role of the Ras Pathway in Enhancing Androgenic Signaling in Prostate Cancer

Androgen receptor (AR) plays an important role in normal prostate function as well as in the etiology of prostate cancer. Activation of AR is dictated by hormone binding and by interactions with co-regulators. Several of these co-regulators are known...

Authors: Mukhopadhyay NK, Cinar B, Mukhopadhyay L, Lutchman M, Ferdinand AS, Kim J, Chung LW, Adam RM, Ray...

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