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4878 results sorted by

Tuberous sclerosis complex proteins control axon formation

Axon formation is fundamental for brain development and function. TSC1 and TSC2 are two genes, mutations in which cause tuberous sclerosis complex (TSC), a disease characterized by tumor predisposition and neurological abnormalities including...
Authors: Choi YJ, Di Nardo A, Kramvis I, Meikle L, Kwiatkowski DJ, Sahin M, He X

Methods for siRNA-mediated Reduction of mRNA and Protein Expression in Human Placental Explants, Isolated Primary Cells and Cell Lines

The use of RNA interference (RNAi) to deplete individual proteins from cells or tissue has revolutionised our ability to characterise gene function. The placenta is an attractive target for studies in which the role of specific proteins can be...
Authors: K. Forbes, M. Desforges, R. Garside, J.D. Aplin, and M. Westwood

Regulation of cartilage-specific gene expression in human chondrocytes by SirT1 and NAMPT

SSirT1 is an NAD-dependent histone deacetylase that regulates gene expression, differentiation, development and organism life span. Here we investigate the function of SirT1 in human chondrocytes derived from osteoarthritic patients. Elevation of...
Authors: Dvir-Ginzberg M, Gagarina V, Lee EJ, Hall DJ

Human induced pluripotent stem cells free of vector and transgene sequences.

Reprogramming differentiated human cells to induced pluripotent stem (iPS) cells has applications in basic biology, drug development, and transplantation. Human iPS cell derivation previously required vectors that integrate into the genome, which can...
Authors: Yu J, Hu K, Smuga-Otto K, Tian S, Stewart R, Slukvin II, Thomson JA.
SST Peer Reviewed

Tribbles-2 is a novel regulator of inflammatory activation of monocytes

Inflammatory activation of monocytes is an essential part of both innate immune responses and the pathogenesis of conditions such as atherosclerosis. However, the mechanisms which modulate the response of monocytes to inflammatory stimuli are still...
Authors: Eder K, Guan H, Sung HY, Ward J, Angyal A, Janas M, Sarmay G, Duda E, Turner M, Dower SK, Francis...

The Anti-Inflammatory Effects of Adiponectin Are Mediated via a Heme Oxygenase-1–Dependent Pathway in Rat Kupffer Cells

Altered expression and activity of immunomodulatory cytokines plays a major role in the pathogenesis of alcoholic liver disease. Chronic ethanol feeding increases the sensitivity of Kupffer cells, the resident hepatic macrophage, to...
Authors: Palash Mandal, Pil-Hoon Park, Megan R. McMullen, Brian T. Pratt, and Laura E. Nagy

Target Gene-Specific Regulation of Androgen Receptor Activity by p42/p44 Mitogen-Activated Protein Kinase

Evidence that the androgen receptor (AR) is not only important in androgen-dependent prostate cancer, but also continues to play a role in tumors that become resistant to androgen deprivation therapies, highlights the need to find alternate means to...
Authors: Agoulnik IU, Bingman WE 3rd, Nakka M, Li W, Wang Q, Liu XS, Brown M, Weigel NL

Gene targeting of a disease-related gene in human induced pluripotent stem and embryonic stem cells.

We report here homologous recombination (HR)-mediated gene targeting of two different genes in human iPS cells (hiPSCs) and human ES cells (hESCs). HR-mediated correction of a chromosomally integrated mutant GFP reporter gene reaches efficiencies of...
Authors: Zou J, Maeder ML, Mali P, Pruett-Miller SM, Thibodeau-Beganny S, Chou BK, Chen G, Ye Z, Park IH,...
SST Peer Reviewed

Transcriptional restriction of human immunodeficiency virus type 1 gene expression in undifferentiated primary monocytes

Monocytes are critical precursors of dendritic cells and macrophages, which play an important role in the pathogenesis of human immunodeficiency virus type 1 (HIV-1). HIV-1 postentry infection is blocked in undifferentiated monocytes in vitro, while...
Authors: Dong C, Kwas C, Wu L

Evaluation of high efficiency gene knockout strategies for Trypanosoma cruzi.

BACKGROUND: Trypanosoma cruzi, a kinetoplastid protozoan parasite that causes Chagas disease, infects approximately 15 million people in Central and South America. In contrast to the substantial in silico studies of the T. cruzi genome,...
Authors: Xu D, Brandܡn CP, Basombrܭo MA, Tarleton RL.
SST Peer Reviewed