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The Role of Different Supplements in Expression Level of Monoclonal Antibody against Human CD20

BACKGROUND: Recombinant monoclonal antibodies have been marketed in last three decades as the major therapeutic proteins against different cancers. However choosing a proper medium and supplements to reach the high expression is a challenging...

Authors: Mahboudi F, Abolhassan MR, Azarpanah A, Aghajani-Lazarjani H, Sadeghi-Haskoo MA, Maleknia S, Vaziri...

Recombinant human butyrylcholinesterase as a new-age bioscavenger drug: development of the expression system

Butyrylcholinesterase (BChE) is a serine hydrolase (EC 3.1.1.8) which can be found in most animal tissues. This enzyme has a broad spectrum of efficacy against organophosphorus compounds, which makes it a prime candidate for the role of...

Authors: Ilyushin DG, Haertley OM, Bobik TV, Shamborant OG, Surina EA, Knorre VD, Masson P, Smirnov IV,...

Derivation of lung epithelium from human cord blood-derived mesenchymal stem cells

RATIONALE: Recent studies have suggested that both embryonic stem cells and adult bone marrow stem cells can participate in the regeneration and repair of diseased adult organs, including the lungs. However, the extent of airway epithelial...

Authors: Sueblinvong V, Loi R, Eisenhauer PL, Bernstein IM, Suratt BT, Spees JL, Weiss DJ.

Peptone Supplementation of Culture Medium Has Variable Effects on the Productivity of CHO Cells

The optimization of cell culture conditions for growth and productivity of recombinant Chinese hamster ovary (CHO) cells is a critical step in biopharmaceutical manufacturing. In the present study, the effects of the timing and amount of peptone...

Authors: Davami F, Baldi L, Rajendra Y, M Wurm F

Benchmarking of commercially available CHO cell culture media for antibody production

In this study, eight commercially available, chemically defined Chinese hamster ovary (CHO) cell culture media from different vendors were evaluated in batch culture using an IgG-producing CHO DG44 cell line as a model. Medium adaptation revealed...

Authors: Reinhart D1 Damjanovic L, Kaisermayer C, Kunert R

Stimulation of Activin A/Nodal signaling is insufficient to induce definitive endoderm formation of cord blood-derived unrestricted somatic stem cells.

NTRODUCTION: Unrestricted somatic stem cells (USSC) derived from umbilical cord blood are an attractive alternative to human embryonic stem cells (hESC) for cellular therapy. USSC are capable of forming cells representative of all three germ line...

Authors: Filby CE, Williamson R, van Kooy P, Pébay A, Dottori M, Elwood NJ, Zaibak F.

Allogeneic mesenchymal precursor cell therapy to limit remodeling after myocardial infarction: the effect of cell dosage

BACKGROUND: This experiment assessed the dose-dependent effect of a unique allogeneic STRO-3-positive mesenchymal precursor cell (MPC) on postinfarction left ventricular (LV) remodeling. The MPCs were administered in a manner that would simulate...

Authors: Hamamoto H, Gorman JH 3rd, Ryan LP, Hinmon R, Martens TP, Schuster MD, Plappert T, Kiupel M, St...

Epithelial cell differentiation of human mesenchymal stromal cells in decellularized lung scaffolds.

Identification of appropriate donor cell types is important for lung cell therapy and for lung regeneration. Previous studies have indicated that mesenchymal stromal cells derived from human bone marrow (hBM-MSCs) and from human adipose tissue...

Authors: Mendez JJ, Ghaedi M, Steinbacher D, Niklason LE.

Transcription factor induction of human oligodendrocyte progenitor fate and differentiation

Human oligodendrocyte progenitor cell (OPC) specification and differentiation occurs slowly and limits the potential for cell-based treatment of demyelinating disease. In this study, using FACS-based isolation and microarray analysis, we identified a...

Authors: Wang 1, Pol SU, Haberman AK, Wang C, O\\\'Bara MA, Sim FJ

Monocyte interaction accelerates HCl-induced lung epithelial remodeling.

BACKGROUND: Acute respiratory distress syndrome (ARDS) is characterized by overwhelming inflammatory responses and lung remodeling. We hypothesized that leukocyte infiltration during the inflammatory response modulates epithelial remodeling through...

Authors: Chen Q, Luo AA, Qiu H, Han B, Ko BH, Slutsky AS, Zhang H

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