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Nurr1-RXR heterodimers mediate RXR ligand-induced signaling in neuronal cells

The retinoid X receptor (RXR) is essential as a common heterodimerization partner of several nuclear receptors (NRs). However, its function as a bona fide receptor for endogenous ligands has remained poorly understood. Such a role would depend on the...

Authors: Wallen-Mackenzie A, De Urquiza AM, Petersson S, Rodriguez FJ, Friling S, Wagner J, Ordentlich P,...

Nucleofection as an efficient nonviral transfection method for human monocytic cells

Despite some progress in the field of gene transfer into hard-to-transfect cells, so far an efficient nonviral method for monocytes has not been available. A comparison of plasmid DNA with capped and polyadenylated mRNA for enhanced green fluorescent...

Authors: Martinet W, Schrijvers DM and Kockx MM

Membrane glucocorticoid receptors (mGCR) are expressed in normal human peripheral blood monunuclear cells and up-regulated after in vitro stimulation and in patients with rheumatoid arthritis

Glucocorticoids mediate their therapeutic actions mostly by genomic effects via cytosolic receptors, but some effects are too rapid to be mediated by changes at the genomic level. The detailed mechanisms of these nongenomic actions are still unclear....

Authors: Bartholome B, Spies CM, Gaber T, Schuchmann S, Berki T, Kunkel D, Bienert M, Radbruch A, Burmester...

Neuron-derived orphan receptor-1 (NOR-1) modulates vascular smooth muscle cell proliferation

Vascular smooth muscle cells (VSMCs) migration and proliferation play a key role in the pathophysiology of cardiovascular disease. However, the transcription factors that regulate VSMC activation are not completely characterized. By a...

Authors: Martinez-Gonzalez J, Rius J, CastellÜ³ A, Cases-Langhoff C and Badimon L

Laforin, the dual-phosphatase responsible for Lafora disease, interacts with R5 (PTG), a regulatory subunit of protein phosphatase-1 that enhances glycogen accumulation

Laforin, the dual-phosphatase responsible for Lafora disease, interacts with R5 (PTG), a regulatory subunit of protein phosphatase-1 that enhances glycogProgressive myoclonus epilepsy of Lafora type (LD, MIM 254780) is a fatal autosomal recessive...

Authors: Fernandez-Sanchez ME, Criado-Garcia O, Heath KE, Garcia-Fojeda B, Medrano-Fernandez I, Gomez-Garre...

Biochemical and functional characterization of Rab27a mutations occurring in Griscelli syndrome patients

Rab27a is a member of the Rab family of small GTPase proteins, and thus far is the first member to be associated with a human disease (ie, the Griscelli syndrome type 2). Mutations in the Rab27a gene cause pigment as well as cytotoxic granule...

Authors: Menasche G, Feldmann J, Houdusse A, Desaymard C, Fischer, Goud B and de Saint Basile G

LIME: A new membrane raft-associated adaptor protein involved in CD4 and CD8 coreceptor signaling

Lymphocyte membrane rafts contain molecules critical for immunoreceptor signaling. Here, we report identification of a new raft-associated adaptor protein LIME (Lck-interacting molecule) expressed predominantly in T lymphocytes. LIME becomes tyrosine...

Authors: Brdickova N, Brdicka T, Angelisova P, Horvath O, Spicka J, Hilgert I, Paces J, Simeoni L, Kliche S,...

Memory and flexibility of cytokine gene expression as separable properties of human TH1 and TH2 lymphocytes

CD4+ T cell priming under T helper type I (T(H)1) or T(H)2 conditions gives rise to polarized cytokine gene expression. We found that in these conditions human naive T cells acquired stable histone hyperacetylation at either the Ifng or Il4 promoter....

Authors: Messi M, Giacchetto I, Nagata K, Lanzavecchia A, Natoli G and Sallusto F

Antitumor activity of lysophosphatidic acid acyltransferase-beta inhibitors, a novel class of agents, in multiple myeloma

In this study, we examined the effects of isoform-specific functional inhibitors of lysophosphatidic acid acyltransferase (LPAAT), which converts lysophosphatidic acid to phosphatidic acid, on multiple myeloma (MM) cell growth and survival. The...

Authors: Hideshima T, Chauhan D, Hayashi T, Podar K, Akiyama M, Mitsiades C, Mitsiades N, Gong B, Bonham L,...

Analysis of intracellular storage and regulated secretion of three von Willebrands disease-causing variants of von Willebrand factor

The rapid exocytosis of von Willebrand factor (VWF) in response to vascular injury can be attributed to the fact that VWF is stored in the Weibel-Palade bodies (WPBs) of endothelial cells. We describe a system for examining the ability of VWF to...

Authors: Michaux G, Hewlett LJ, Messenger SL, Goodeve AC, Peake IR, Daly ME and Cutler DF

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