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Mitochondrial dysfunction induces triglyceride accumulation in 3T3-L1 cells: role of fatty acid -oxidation and glucose

Mitochondrial cytopathy has been associated with modifications of lipid metabolism in various situations, such as the acquisition of an abnormal adipocyte phenotype observed in multiple symmetrical lipomatosis or triglyceride (TG) accumulation in...

Authors: Vankoningsloo S, Piens M, Lecocq C, Gilson A, De Pauw A, Renard P, Demazy C, Houbion A, Raes M and...

Effective gene delivery to adult neurons by a modified form of electroporation

Non-viral methods of transfection of cDNAs into adult neurons and other post-mitotic cells are generally very inefficient. However, the recent development of Nucleofectortrade mark technology developed by Amaxa Biosystems allows direct delivery of...

Authors: Leclere PG, Panjwani A, Docherty R, Berry M, Pizzey J, Tonge DA

Cross-presentation by intercellular peptide transfer through gap junctions

Major histocompatibility complex (MHC) class I molecules present peptides that are derived from endogenous proteins. These antigens can also be transferred to professional antigen-presenting cells in a process called cross-presentation, which...

Authors: Neijssen J, Herberts C, Drijfhout JW, Reits E, Janssen L and Neefjes J

Up-regulation of the lymphatic marker podoplanin, a mucin-type transmembrane glycoprotein, in human squamous cell carcinomas and germ cell tumors

The mucin-type glycoprotein podoplanin is specifically expressed by lymphatic but not blood vascular endothelial cells in culture and in tumor-associated lymphangiogenesis, and podoplanin deficiency results in congenital lymphedema and impaired...

Authors: Schacht V, Dadras SS, Johnson LA, Jackson DG, Hong YK and Detmar M

Essential role of TRPC channels in the guidance of nerve growth cones by brain-derived neurotrophic factor

Brain-derived neurotrophic factor (BDNF) is known to promote neuronal survival and differentiation and to guide axon extension both in vitro and in vivo. The BDNF-induced chemo-attraction of axonal growth cones requires Ca(2+) signalling, but how...

Authors: Li Y, Jia YC, Cui K, Li N, Zheng ZY, Wang YZ and Yuan XB

Sphingosine kinase-1 enhances endothelial cell survival through a PECAM-1-dependent activation of PI-3K/Akt and regulation of Bcl-2 family members

SSphingosine-1-phosphate (S1P), the bioactive product of sphingosine kinase (SK) activation is a survival factor for endothelial cells. The mechanism of SK mediated survival was investigated in endothelial cells with moderately raised intracellular...

Authors: Limaye VS, Li X, Hahn C, Xia P, Berndt MC, Vadas MA and Gamble JR

Involvement of BLT1 Endocytosis and Yes Kinase Activation in Leukotriene B4-Induced Neutrophil Degranulation

One of the important biological activities of human neutrophils is degranulation, which can be induced by leukotriene B(4) (LTB(4)). Here we investigated the intracellular signaling events involved in neutrophil degranulation mediated by the high...

Authors: Gaudreault E, Thompson C, Stankova J and Rola-Pleszczynski M

c-Myc associates with ribosomal DNA and activates RNA polymerase I transcription

The c-Myc oncoprotein regulates transcription of genes that are associated with cell growth, proliferation and apoptosis. c-Myc levels are modulated by ubiquitin/proteasome-mediated degradation. Proteasome inhibition leads to c-Myc accumulation...

Authors: Arabi A, Wu S, Ridderstrale K, Bierhoff H, Shiue C, Fatyol K, Fahlen S, Hydbring P, Soderberg O,...

Different MHC Class I Heavy Chains Compete with Each Other for Folding Independently of 2-Microglobulin and Peptide

We reported previously that different MHC class I molecules can compete with each other for cell surface expression in F(1) hybrid and MHC class I transgenic mice. In this study, we show that the competition also occurs in transfected cell lines, and...

Authors: Tourdot S, Nejmeddine M, Powis SJ and Gould KG

beta-arrestin Dependent Spontaneous 1a-Adrenoceptor Endocytosis causes Intracellular Transportation of -blockers via Recycling Compartments

The antagonist ligand BODIPY-FL-prazosin (QAPB) fluoresces when bound to bovine alpha1a-adrenoceptors (ARs). Data indicates that the receptor-ligand complex is spontaneously internalized by beta-arrestin-dependent endocytosis. Internalization of the...

Authors: Pediani JD, Colston JF, Caldwell D, Milligan G, Daly CJ, McGrath JC

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